Fabrice Dessaint

To better understand the changes in weed communities following the adoption of conservation agriculture (CA), we investigated the functional composition of weed communities in fields implementing CA continuously from 1 to 21 years. Weed surveys were conducted in 100 winter wheat fields of 53 farmers in the Bourgogne‐Franche‐Comté region, Eastern France. Ten response traits were selected to highlight changes in the functional composition of weed communities, using weighted mean value for quantitative traits and an approach based on composition analysis for qualitative traits. Out of the 10 traits, four were impacted by the time following the adoption of CA significantly, whereas two only showed trends. Specific leaf area, leaf dry matter content, seed dispersal mode and soil seedbank longevity did not change over time following adoption of CA, whereas changes were observed for plant type, Raunkiær's life forms, Ellenberg's indicator value for light and, germination and flowering periods. Phanerophyte and geophyte life forms, summer‐germinating species and spring‐ and summer‐flowering species increased with time following adoption of CA. Shade‐tolerant species, not present in fields recently converted to CA, established later. Community‐weighted mean of seed mass and the percentage of graminoids tended to increase with time following adoption of CA. Some functional shifts in weed communities occurred quicker than others (i.e., summer‐germinating species increased after at least 10 years of continuous application of CA principles). These results can be used to inform farmers moving towards CA about expected changes.

Phelipanche ramosa is a major weed holoparasite characterized by a broad host range with a suboptimal development on numerous hosts, suggesting inter- or intra-species specificities. Seeds of P. ramosa germinate after exposure to exogenous chemicals exuded by surrounding host roots such as strigolactones, the concentrations of these germination stimulants varying between hosts. In France, P. ramosa is characterized by genetically differentiated populations presenting varying germination rates and a host specificity. The objective of our study was to investigate the sensitivity of seeds of two P. ramosa populations harvested on tobacco and oilseed rape, to a set of GR24 concentrations, a synthetic strigol analogue. The assessment of the germination rate was based on in vitro experiments. Seeds of P. ramosa were placed in Petri dishes with various concentrations of GR24. The cumulative number of germinated seeds of P. ramosa was counted several times after application of the treatment. Cumulative germination curves were analysed using a three-parameter log-logistic model and a time-to-event approach. The results show that the germination rate of P. ramosa seeds depends on the GR24 concentration and the duration of stimulation, but also that the response to these two factors varies greatly according to the origin of the P. ramosa seeds. The difference in germination speed between P. ramosa populations further shows distinct responses at the intraspecific level, thus suggesting that the specialization of P. ramosa probably occurs at least from the first stage of the holoparasite cycle.

Rainfed lowland rice fields in northern Togo are increasingly infested by the facultative hemiparasitic weed Rhamphicarpa fistulosa that is widely reported throughout sub‐Saharan Africa to be one of the most damaging weeds in rice fields. In this geographical area, some studies have shown that soil characteristics may influence the presence and/or the level of infestation of some parasitic weeds, but none have been conducted on R. fistulosa. Based on surveys conducted in northern Togo over two years in 66 lowland rice fields, we characterised the level of infestation of R. fistulosa as well as physico‐chemical characteristics of soils. We highlighted that fields with medium‐to‐high levels of infestation of R. fistulosa (more than 5 plants per m²) were mainly characterised by a coarser soil texture, a higher potassium content and a higher pH, while fields without R. fistulosa were characterised by a lower proportion of silt content. In contrast, the level of infestation of R. fistulosa appeared only marginally influenced by soil organic carbon and nitrogen in our study. Considering these results, options to modify the main physico‐chemical characteristics of soils that influence the level of infestation of R. fistulosa are likely difficult to implement. In order to continue rice production in these lowland fields, we suggest several measures to better manage R. fistulosa seedbank and development.

Recently adopted in France, conservation agriculture still faces some challenges to its adoption, particularly weed management. To highlight the weed management practices used by farmers in conservation agriculture and the changes induced by its adoption, a large sample of 425 French farmers were invited to complete an online survey. Weed management practices used by farmers were requested for three periods: before adoption, during the first years of conservation agriculture (one to two years after adoption), and when the agricultural system is considered “mastered” by the farmer. The use of each farming practice was firstly studied independently for each period. Then, a multiple correspondence analysis followed by a hierarchical ascendant classification resulted in groups of farmers with different combinations of practices for each period. Finally, the groups of farmers were followed through the periods. Results showed that changes in weed management done according to farmers when adopting and mastering conservation agriculture are multiple and vary according to farmers and their previous weed management. Although some similar choices were identified, some farmers’ trajectories, especially those with a prior combination driven by either a soil disturbance strategy or a crop competitiveness strategy, are difficult to identify when adopting conservation agriculture. Upon mastering the agricultural system, farmers’ choices become more apparent.

Le semis direct sous couvert repose sur l’application simultanée et continue de trois principes : la réduction quasi-totale du travail du sol, une couverture organique des sols et une rotation diversifiée. Ce système agricole adopté en France à partir des années 2000 sous l’impulsion de groupes d’agriculteurs est en extension. Pour les agriculteurs, adopter un nouveau système agricole revient à modifier, de manière plus ou moins importante, leurs pratiques. Concernant le semis direct sous couvert végétal, peu d’informations existent. Cet article a pour objectif d’éclairer les modifications que peut induire sa mise en place. Pour cela, il s’appuie sur les réponses de 425 agriculteurs français à une enquête en ligne. Pour 30 % des agriculteurs, le semis direct sous couvert constitue une modification complète du système agricole. La réduction quasi-totale du travail du sol est le principe qui entraîne le plus de modifications (pour 96 % des agriculteurs). Au contraire, la diversification de la rotation connaît le moins de modifications (48 % des agriculteurs). L’absence d’une modification de la rotation peut s’expliquer par une rotation déjà diversifiée en place ou l’incapacité pour les agriculteurs d’ajouter de nouvelles cultures à leur rotation. L’optimisation de la couverture végétale du sol entraîne une modification des pratiques pour 67 % des agriculteurs. Durant les premières années, les agriculteurs s’orientent majoritairement vers l’utilisation de couverts temporaires plurispécifiques. Bouleversant certains fondamentaux de l’agriculture, ces agriculteurs minimisent les risques encourus en favorisant une transition progressive et en partageant les connaissances acquises.

Phelipancheramosa is a major root-holoparasitic damaging weed characterized by a broad host range, including numerous Fabaceae species. In France, the agricultural threat posed by P. ramosa has increased over two decades due to the appearance of a genetically differentiated pathovar presenting a clear host specificity for oilseed rape. The new pathovar has led to a massive expansion of P. ramosa in oilseed rape fields. The germination rate of P. ramosa seeds is currently known to vary among P. ramosa pathovars and host species. However, only a few studies have investigated whether phylogenetic relatedness among potential host species is a predictor of the ability of these species to induce the seed germination of parasitic weeds by testing for phylogenetic signal. We focused on a set of 12 Fabaceae species and we assessed the rate of induction of seed germination by these species for two pathovars based on in vitro co-cultivation experiments. All Fabaceae species tested induced the germination of P. ramosa seeds. The germination rate of P. ramosa seeds varied between Fabaceae species and tribes studied, while pathovars appeared non-influential. Considering oilseed rape as a reference species, we also highlighted a significant phylogenetic signal. Phylogenetically related species therefore showed more similar rates of induction of seed germination than species drawn at random from a phylogenetic tree. In in vitro conditions, only Lotus corniculatus induced a significantly higher germination rate than oilseed rape, and could potentially be used as a catch crop after confirmation of these results under field conditions.

Achieving sustainable crop production while feeding an increasing world population is one of the most ambitious challenges of this century¹. Meeting this challenge will necessarily imply a drastic reduction of adverse environmental effects arising from agricultural activities². The reduction of pesticide use is one of the critical drivers to preserve the environment and human health. Pesticide use could be reduced through the adoption of new production strategies^3,​4,​5; however, whether substantial reductions of pesticide use are possible without impacting crop productivity and profitability is debatable^{6,​7,​8,​9,​10,​11,​12,​13,​14,​15,​16,​17}. Here, we demonstrated that low pesticide use rarely decreases productivity and profitability in arable farms. We analysed the potential conflicts between pesticide use and productivity or profitability with data from 946 non-organic arable commercial farms showing contrasting levels of pesticide use and covering a wide range of production situations in France. We failed to detect any conflict between low pesticide use and both high productivity and high profitability in 77% of the farms. We estimated that total pesticide use could be reduced by 42% without any negative effects on both productivity and profitability in 59% of farms from our national network. This corresponded to an average reduction of 37, 47 and 60% of herbicide, fungicide and insecticide use, respectively. The potential for reducing pesticide use appeared higher in farms with currently high pesticide use than in farms with low pesticide use. Our results demonstrate that pesticide reduction is already accessible to farmers in most production situations. This would imply profound changes in market organization and trade balance.

Human activities lead to a process of homogenization of biotas in which specialist species are increasingly replaced by common and widespread species. Using a 30-year diachronic record of arable weed communities, we tested this hypothesis by quantifying changes in α- and β-diversity, using both taxonomic and functional diversity and by partitioning β-diversity into species replacement and richness differences. Arable weed communities were sampled in the same 158 fields of the Côte-d’Or region (northeastern France) between the 1970s and the 2000s. For each period, each field was characterized by crop types, soil characteristics and a High Nature Value (HNV) farmland index based on agricultural intensification at the landscape level. At the field scale, we observed a loss of 46% and 38% in α-taxonomic and functional diversity, respectively, which was in accordance with the decrease in the HNV farmland index over the same period. At the regional scale, there was an increase of 15% and 21% in β-taxonomic and functional diversity (across fields), respectively. Crop type and soil characteristics explained similar levels of variation in species replacement, and crop type explained much larger richness differences in the 2000s suggesting that crop and associated practices may exert a high filtering effect. Our results also highlighted a marked decline of common weeds; a process that is far from being counterbalanced by the few colonizing weeds. Rather than to biotic homogenization, this pattern of loss has led to a higher differentiation of arable weed communities. This could correspond to a fragmentation of suitable habitats for species that depend on weeds. This pattern was associated with a decrease of species richness per field; the loss of common species and their associated functions may be of greater significance for agroecosystem functioning.