Katalin Bajer

Theory of sexual selection states that males often develop showy signals, which reduce their survival but increase their reproductive success. During mate choice, these conspicuous signals can be honest indicators of individual quality conveying information about the signaler's physiological state. Sexually selected signals are influenced by many environmental factors; however, whether signals and physiological state are affected together is rarely studied. The ultraviolet‐blue throat color of male Lacerta viridis is an intra‐ and intersexually selected signal connected to blood parasite infection and influenced by environmental factors. The aim of this study was to experimentally investigate how ecologically relevant environmental factors affect color signal intensity and key physiological traits parallel. During the mating season, we exposed 40 adult male lizards infected with blood parasites to food and basking time treatments in a full factorial design. We measured color, amount of reactive oxygen metabolites (ROMs), hematocrit, immunocompetence, and blood parasite intensity before and after treatments. High basking time resulted in elevated immunocompetence coupled with increased ROMs. The high food treatment increased nuptial color brightness, but also increased ROMs and decreased immunocompetence. In summary, our study provides experimental evidence about environmentally induced parallel changes in an honest sexual signal and several quality‐indicator physiological traits. We showed that available energy and time for high metabolism have independent and sometimes opposite effects on individual state.

Nuptial traits signalling individual quality are common in numerous animal taxa, and play a significant role in sexual selection. Detecting female mate choice based on visual cues is notoriously hard in lizards. Previously, we found that female European green lizards (Lacerta viridis) preferred to associate with males with high ultraviolet (UV) throat reflectance. Here, we investigated if different components of nuptial throat colour of male European green lizards were correlated to other fitness‐related traits, and thus could signal male quality. We found that (1) high UV chroma correlates positively with directional asymmetry and shows a negative trend with body condition; (2) blue chroma is not related to any individual traits; and (3) total throat brightness correlates positively with body size and relative head size, and negatively with ectoparasite load. Our results suggest that having high throat UV reflectance is costly for male European green lizards, so probably only high‐quality individuals can afford it, while total brightness of the throat colour signals age, relative head size and health status. Hence, throat colour in male European green lizards is a multiple honest signal.

Colour signals are widely used in intraspecific communication and often linked to individual fitness. The development of some pigment-based (e.g. carotenoids) colours is often environment-dependent and costly for the signaller, however, for structural colours (e.g. ultraviolet [UV]) this topic is poorly understood, especially in terrestrial ectothermic vertebrates. In a factorial experiment, we studied how available energy and time at elevated body temperature affects the annual expression of the nuptial throat colour patch in male European green lizards (Lacerta viridis) after hibernation and before mating season. In this species, there is a female preference for males with high throat UV reflectance, and males with high UV reflectance are more likely to win fights. We found that (i) while food shortage decreased lizards' body condition, it did not affect colour development, and (ii) the available time for maintaining high body temperature affected the development of UV colour without affecting body condition or other colour traits. Our results demonstrate that the expression of a sexually selected structural colour signal depends on the time at elevated body temperature affecting physiological performance but not on available energy gained from food per se in an ectothermic vertebrate. We suggest that the effect of high ambient temperature on UV colour in male L. viridis makes it an honest signal, because success in acquiring thermally favourable territories and/or effective behavioural thermoregulation can both be linked to individual quality.

Animal communication through colour signals is a central theme in sexual selection. Structural colours can be just as costly and honest signals as pigment-based colours. Ultraviolet (UV) is a structural colour that can be important both in intrasexual competition and mate choice. However, it is still unknown if a UV signal alone can determine the outcome of male–male fights. European green lizard (Lacerta viridis) males develop a nuptial throat coloration with a strong UV component. Among males differing only in their manipulated UV colour, females prefer males with higher UV. Here, we experimentally decreased the UV coloration of randomly chosen males from otherwise similar male pairs to test the hypothesis that a difference in UV colour alone can affect fight success during male–male competition. Our results fully supported the hypotheses: in almost 90 per cent of the contests the male with reduced UV lost the fight. Our results show that UV can be an important signal, affecting both female mate choice and determining male fight success.