Alice Ma

IPI-926 treatment alters cellular composition in the tumor microenvironment.

Myeloid network.Regulatory network for PDAC-associated myeloid cells, listing the inferred transcriptional targets (Target) for each regulatory protein (Regulator). Association weight (AW) and association mode (AM) are scores to quantify strength/direction of interaction. Sign indicates directionality of the interaction (1 = transactivating, -1 = transrepressing).

Abrogation of SHH signaling fuels hypersprouting in human and murine PDAC explants.

Overview of single cell regulatory network analysis.

Explant cellular populations throughout culture.

Supplementary Table 10: Antibodies for IHC and IF.Overview of antibodies used for stainings in human PDAC or KPC-derived tissues.Supplementary Table 11: Primer sequences for qRT-PCR.Primers for qRT-PCR-based quantification of ChIP and mRNA samples.Supplementary Table 12: Freezer dryer settings for sponge production.Specific settings for optimal sponge production.Supplementary Table 13: Explant media composition.List of reagents used for human PDAC and murine explants.

Explant cellular populations throughout culture.

Supplementary Table 1: Raw read counts per gene for bulk RNA-seq IPI-926 intervention study.Raw read counts of matching biopsy (Bx) and necropsy (Nx) bulk RNA-seq samples after two days of treatment with vehicle or IPI-926.Supplementary Table 2: Digestion buffer for single cell preparation.List of reagents optimized for single cell digestion of KPC-derived tumors.Supplementary Table 3: Single Cell RNA-seq Quality Control.Overview of KPC-derived scRNA-seq samples, containing treatment information (vehicle or IPI-926; n=3 each), sequencing depth, detected genes, and detection of mitochondrial genes (MT%).Supplementary Table 4: Network sizes in murine scRNA-seq data.Number of regulatory proteins for which regulons were generated in each network type, in each cell type, from KPC mouse pancreatic tumors.

SHH secretion in vitro and ex vivo.