Mikiko Matsui
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Mikiko Matsui has a total of 2 co-authors. Here are the top co-authors by number of shared publications:
- Takeo W. Tsutsui
Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan
1 shared publication - Tomoko Kobayashi
Research Center for Odontology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan
1 shared publication
Latest Publications
- Open Access
- 8 January 2018
- Published by Springer Nature in Human Cell
- Vol. 31 (2) , 127-138
CD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures
CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146+) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146−) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146+ and CD146− cells, as well as mixtures composed of 25% CD146+ cells and 75% CD146− cells (CD146+/−). Cell growth assays indicated that CD146+ cells exhibit an approximate 3–4 h difference in doubling time, compared with CD146− cells. Cell cycle distributions were determined by flow cytometry analysis. The low percentage of CD146+ cells’ DNA content in G0/G1 phase were compared with CD146− and non-separated cells. In contrast to CD146− and non-separated cells, prompt mineralization was observed in CD146+ cells. Subsequently, qRT-PCR revealed high mRNA expression of CD146 and Alkaline phosphatase in mineralization-induced CD146+ cells. CD146+ cells were also observed high adipogenic ability by Oil red O staining. Histological examinations revealed an increased area of dentin/pulp-like structures in transplanted CD146+ cells, compared with CD146− and CD146+/− cells. Immunohistochemical studies detected dentin matrix protein-1 (DMP1) and dentin sialophosphoprotein (DSPP), as well as human mitochondria, in transplanted DPSCs. Co-expression of CD146 and GFP indicated that CD146 was expressed in transplanted CD146+ cells. CD146+ cells may promote mineralization and generate dentin/pulp-like structures, suggesting a role in self-renewal of stem cells and dental pulp regenerative therapy.
- 8 January 2018
- Published by Springer Nature in Human Cell
- Vol. 31 (2) , 127-138
Open AccessCD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures
CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146+) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146−) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146+ and CD146− cells, as well as mixtures composed of 25% CD146+ cells and 75% CD146− cells (CD146+/−). Cell growth assays indicated that CD146+ cells exhibit an approximate 3–4 h difference in doubling time, compared with CD146− cells. Cell cycle distributions were determined by flow cytometry analysis. The low percentage of CD146+ cells’ DNA content in G0/G1 phase were compared with CD146− and non-separated cells. In contrast to CD146− and non-separated cells, prompt mineralization was observed in CD146+ cells. Subsequently, qRT-PCR revealed high mRNA expression of CD146 and Alkaline phosphatase in mineralization-induced CD146+ cells. CD146+ cells were also observed high adipogenic ability by Oil red O staining. Histological examinations revealed an increased area of dentin/pulp-like structures in transplanted CD146+ cells, compared with CD146− and CD146+/− cells. Immunohistochemical studies detected dentin matrix protein-1 (DMP1) and dentin sialophosphoprotein (DSPP), as well as human mitochondria, in transplanted DPSCs. Co-expression of CD146 and GFP indicated that CD146 was expressed in transplanted CD146+ cells. CD146+ cells may promote mineralization and generate dentin/pulp-like structures, suggesting a role in self-renewal of stem cells and dental pulp regenerative therapy.