Joshua R Sanes
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Joshua R Sanes has a total of 4 co-authors. Here are the top co-authors by number of shared publications:
- Dawen Cai
Department of Cell and Developmental Biology, University of Michigan
1 shared publication Jeff W Lichtman
Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
1 shared publicationKimberly B Cohen
Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
1 shared publicationTuanlian Luo
Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
1 shared publication
Latest Publications
- Top Cited PapersOpen Access
- 5 May 2013
- Published by Springer Nature in Nature Methods
- Vol. 10 (6) , 540-547
Improved tools for the Brainbow toolbox
An improved Brainbow toolbox for expression in the mouse is presented in this Resource. The collection includes transgenic lines, plasmids and viral vectors with improved performance and added capabilities relative to the original Brainbow constructs. In the transgenic multicolor labeling strategy called 'Brainbow', Cre-loxP recombination is used to create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors. This method has been adapted to non-neuronal cells in mice and to neurons in fish and flies, but its full potential has yet to be realized in the mouse brain. Here we present several lines of mice that overcome limitations of the initial lines, and we report an adaptation of the method for use in adeno-associated viral vectors. We also provide technical advice about how best to image Brainbow-expressing tissue.
- 5 May 2013
- Published by Springer Nature in Nature Methods
- Vol. 10 (6) , 540-547
Top Cited PapersOpen AccessImproved tools for the Brainbow toolbox
An improved Brainbow toolbox for expression in the mouse is presented in this Resource. The collection includes transgenic lines, plasmids and viral vectors with improved performance and added capabilities relative to the original Brainbow constructs. In the transgenic multicolor labeling strategy called 'Brainbow', Cre-loxP recombination is used to create a stochastic choice of expression among fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors. This method has been adapted to non-neuronal cells in mice and to neurons in fish and flies, but its full potential has yet to be realized in the mouse brain. Here we present several lines of mice that overcome limitations of the initial lines, and we report an adaptation of the method for use in adeno-associated viral vectors. We also provide technical advice about how best to image Brainbow-expressing tissue.