Kazumi Tamaki
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Kazumi Tamaki has a total of 5 co-authors. Here are the top co-authors by number of shared publications:
- Chie Ishikawa
Department of Microbiology and Oncology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan
1 shared publication - Koh Tomimori
Department of Microbiology and Oncology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa, 903-0215, Japan
1 shared publication - Naoki Mori
Department of Microbiology and Oncology, Graduate School of Medicine University of the Ryukyus Ginowan Japan
1 shared publication - Ryuichiro Kimura
Department of Microbiology and Oncology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan
1 shared publication - Shinji Nakama
Musashino Research Institute for Immunity, 790 Nishizatozoe, Gusukube, Miyako Island, Okinawa, 906-0106, Japan
1 shared publication
Latest Publications
- Open Access
- 21 June 2012
- Published by Springer Nature in BMC Complementary and Alternative Medicine
- Vol. 12 (1) , 78
Antitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides
Background: Crassocephalum crepidioides, a plant distributed in Okinawa Islands, is known in folk medicine; however, its anticancer activity has not been investigated. The aim of this study was to determine thein vitroandin vivoantitumor activities ofC. crepidioideson murine Sarcoma 180 (S-180) and related molecular mechanisms.Methods: The antitumor effect ofC. crepidioideswas evaluated in S-180-cell-bearing mice. Cell growth was assessed using a colorimetric assay. Nitrite and nitrate levels were measured by colorimetry. The expression levels of inducible NO synthase (iNOS) in murine RAW264.7 macrophages was assessed by reverse transcriptase-polymerase chain reaction. Activation of iNOS promoter was detected by reporter gene. Activation of nuclear factor-κB (NF-κB) was evaluated by electrophoretic mobility shift assay. The role of NF-κB signaling was analyzed using inhibitors of NF-κB and dominant-negative mutants, and Western blot analysis.Results: C. crepidioidesextract delayed tumor growth in S-180-bearing mice. However, it did not inhibit S-180 cell growthin vitro. Supernatant of culturedC. crepidioides-stimulated RAW264.7 macrophages was cytotoxic to S-180 cells. This cytotoxicity was associated with nitric oxide (NO) production. NF-κB signaling pathway was crucial for the transcriptional activation of iNOS gene. Isochlorogenic acid, a component ofC. crepidioides, induced NF-κB activation and iNOS expression.Conclusions: The results highlight the oncolytic and immunopotentiation properties ofC. crepidioidesmediated through NF-κB-induced release of NO from macrophages.
- 21 June 2012
- Published by Springer Nature in BMC Complementary and Alternative Medicine
- Vol. 12 (1) , 78
Open AccessAntitumor activity and macrophage nitric oxide producing action of medicinal herb, Crassocephalum crepidioides
Background: Crassocephalum crepidioides, a plant distributed in Okinawa Islands, is known in folk medicine; however, its anticancer activity has not been investigated. The aim of this study was to determine thein vitroandin vivoantitumor activities ofC. crepidioideson murine Sarcoma 180 (S-180) and related molecular mechanisms.Methods: The antitumor effect ofC. crepidioideswas evaluated in S-180-cell-bearing mice. Cell growth was assessed using a colorimetric assay. Nitrite and nitrate levels were measured by colorimetry. The expression levels of inducible NO synthase (iNOS) in murine RAW264.7 macrophages was assessed by reverse transcriptase-polymerase chain reaction. Activation of iNOS promoter was detected by reporter gene. Activation of nuclear factor-κB (NF-κB) was evaluated by electrophoretic mobility shift assay. The role of NF-κB signaling was analyzed using inhibitors of NF-κB and dominant-negative mutants, and Western blot analysis.Results: C. crepidioidesextract delayed tumor growth in S-180-bearing mice. However, it did not inhibit S-180 cell growthin vitro. Supernatant of culturedC. crepidioides-stimulated RAW264.7 macrophages was cytotoxic to S-180 cells. This cytotoxicity was associated with nitric oxide (NO) production. NF-κB signaling pathway was crucial for the transcriptional activation of iNOS gene. Isochlorogenic acid, a component ofC. crepidioides, induced NF-κB activation and iNOS expression.Conclusions: The results highlight the oncolytic and immunopotentiation properties ofC. crepidioidesmediated through NF-κB-induced release of NO from macrophages.