Jonathan Betz
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Jonathan Betz has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Andrey A. Ptitsyn
Sidra Medical and Research Center, PO Box 26999, Doha, Qatar
1 shared publication - Erica Suchman
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523
1 shared publication - Guadalupe Reyes-Solis
Universidad Autonoma de Yucatan
1 shared publication - Jonathan O. Carlson
Department of Microbiology, Immunology and Pathology, College of Veterinary and Biomedical Sciences, Colorado State University, Fort Collins, CO 80525, USA
1 shared publication - Karla Saavedra-Rodriguez
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
1 shared publication - William Cormack Black
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA
1 shared publication
Latest Publications
- Open Access
- 17 March 2011
- Published by Springer Nature in BMC Genomics
- Vol. 12 (1) , 153
Rhythms and synchronization patterns in gene expression in the Aedes aegypti mosquito
Background: Aedes aegyptiis arguably the most studied of all mosquito species in the laboratory and is the primary vector of both Dengue and Yellow Fever flaviviruses in the field. A large number of transcriptional studies have been made in the species and these usually report transcript quantities observed at a certain age or stage of development. However, circadian oscillation is an important characteristic of gene expression in many animals and plants, modulating both their physiology and behavior. Circadian gene expression in mosquito species has been previously reported but for only a few genes directly involved in the function of the molecular clock.Results: Herein we analyze the transcription profiles of 21,494 messenger RNAs using anAe. aegyptiAgilent®microarray. Transcripts were quantified in adult female heads at 24 hours and then again at 72 hours and eight subsequent time points spaced four hours apart. We document circadian rhythms in multiple molecular pathways essential for growth, development, immune response, detoxification/pesticide resistance. Circadian rhythms were also noted in ribosomal protein genes used for normalization in reverse transcribed PCR (RT-PCR) to determine transcript abundance. We report pervasive oscillations and intricate synchronization patterns relevant to all known biological pathways.Conclusion: These results argue strongly that transcriptional analyses either need to be made over time periods rather than confining analyses to a single time point or development stage or exceptional care needs to be made to synchronize all mosquitoes to be analyzed and compared among treatment groups.
- 17 March 2011
- Published by Springer Nature in BMC Genomics
- Vol. 12 (1) , 153
Open AccessRhythms and synchronization patterns in gene expression in the Aedes aegypti mosquito
Background: Aedes aegyptiis arguably the most studied of all mosquito species in the laboratory and is the primary vector of both Dengue and Yellow Fever flaviviruses in the field. A large number of transcriptional studies have been made in the species and these usually report transcript quantities observed at a certain age or stage of development. However, circadian oscillation is an important characteristic of gene expression in many animals and plants, modulating both their physiology and behavior. Circadian gene expression in mosquito species has been previously reported but for only a few genes directly involved in the function of the molecular clock.Results: Herein we analyze the transcription profiles of 21,494 messenger RNAs using anAe. aegyptiAgilent®microarray. Transcripts were quantified in adult female heads at 24 hours and then again at 72 hours and eight subsequent time points spaced four hours apart. We document circadian rhythms in multiple molecular pathways essential for growth, development, immune response, detoxification/pesticide resistance. Circadian rhythms were also noted in ribosomal protein genes used for normalization in reverse transcribed PCR (RT-PCR) to determine transcript abundance. We report pervasive oscillations and intricate synchronization patterns relevant to all known biological pathways.Conclusion: These results argue strongly that transcriptional analyses either need to be made over time periods rather than confining analyses to a single time point or development stage or exceptional care needs to be made to synchronize all mosquitoes to be analyzed and compared among treatment groups.