Sandra Davi Traverso

Environmental contamination by heavy metals, particularly fish poisoning, is a growing concern due to its impact on aquatic ecosystems. Fish serve as bioindicators and play a crucial role in the food chain. This study evaluated the toxic effects of acute copper and lead exposure in Ctenopharyngodon idella, analyzing hematological, biochemical, and pathological alterations. Fifty-six grass carp were exposed for 96 h to copper and lead (2, 3, and 4 mg/L). Blood samples were analyzed for hematological and oxidative stress parameters, while brain, gills, liver, and kidneys were assessed for enzymatic activity (acetylcholinesterase, catalase, glutathione S-transferase) and histopathology. Data were analyzed using ANOVA and Tukey’s test (p < 0.05). Significant increases in gill glutathione S-transferase (GST) activity (Cu2 and Cu3, p < 0.05) and catalase activity (Pb3 e Pb4, p < 0.05) indicated oxidative stress. Lead exposure caused a significant reduction in erythrocyte count (Pb2, p < 0.05). Although not statistically significant, erythrocyte osmotic fragility increased at higher metal concentrations. Histopathology revealed epithelial hyperplasia, inflammatory infiltrates, and edema in gills, hepatocellular degeneration, and subcapsular/interstitial renal hemorrhages in all exposed groups. These renal lesions diverge from typical heavy metal nephrotoxicity, usually characterized by tubular degeneration. These findings highlight the systemic effects of copper and lead, particularly their oxidative impact and unexpected renal damage. These findings underscore the complex toxicopathological effects of copper and lead in fish, particularly their impact on oxidative stress.

This article introduces a new MfEIT UDESC Mark I system, which consists of a 32-electrode setup featuring a modified Howland current source, low cost, portability, and non-radiation. The system is capable of reconstructing electrical conductivity tomographic images at a rate of 30.624 frames per second, taking about 5 min for imaging. The current source employs a 0.5 mA adjacent current application pattern with frequencies ranging from 10 kHz to 1 MHz. This article outlines the hardware, firmware, and software design specifications, which include the design of the current source, calibration procedures, and image reconstruction process. Tomographic images of conductivity were reconstructed in ex vivo healthy pig lungs and those with pneumonia, as a proof of concept for future applications in live pigs. The high spectral power density, combined with real-time system calibration provides clinical advantages in veterinary medicine. The goal is to identify lung areas affected by Mycoplasma hyopneumoniae in pigs through the analysis of electrical conductivity difference, offering a valuable tool to assist veterinarians to obtain images of respiratory diseases. The modified reconstruction method GREIT (EIDORS) was evaluated with experimental data and was compared with the Gauss–Newton and Total Variation methods, where GREIT 2D proved to be superior.

This article introduces a new MfEIT UDESC Mark I system, which consists of a 32-electrode setup featuring a modified Howland current source, low cost, portable and non radiation. The system is capable of reconstruction of electrical conductivity tomographic images at a rate of 30.624 frames per second and about 5 minutes for imaging. The current source employs 0.5 mA, adjacent current application pattern with frequencies ranging from 10 kHz to 1 MHz. The article outlines the hardware, firmware, and software design specifications, which include the design of the current source, calibration procedures, and image reconstruction process. Tomographic images of conductivity were reconstructed in ex vivo healthy pig lungs and those with pneumonia, as a proof of concept for future applications in live pigs. The high spectral power density, combined with real-time system calibration provides clinical advantages in veterinary medicine. The goal is to identify lung areas affected by Mycoplasma hyopneumoniae in pigs, through the analysis of electrical conductivity difference, offering a valuable tool to assist veterinarians for to obtain images of respiratory diseases. The reconstruction method (GREIT) was evaluated with experimental data and It was compared with the Gauss-Newton and Total Variation methods, where GREIT proved to be superior.

The state of Santa Catarina is the largest national producer of onions. The consumption of onion (Allium cepa) by domestic animals can cause hemolytic anemia and hyperhemoglobinemia of Heinz bodies in erythrocytes. The epidemiological data and the pathological clinical picture were obtained during a visit to the property where the disease occurred. A herd of 54 beef cattle from a property in the municipality of Bom Retiro/SC, was placed in an onion crop, which had not been completely harvested because it was outside the desired standard for trade. The animals remained in this field for eight days. At the end of this period, all of them presented apathy, anorexia, brown urine and mucous membranes and an onion odor on expiration. The macroscopic changes observed were pale mucous membranes, blood with decreased viscosity and increased clotting time, liver with an evident lobular pattern and remains of onion in the rumen. The carcass gave off a strong onion odor. By histological analysis, there was central lobular coagulation necrosis in bridging in the liver. In the kidneys, there were intracytoplasmic hyaline droplets in tubular epithelial cells and a deposit of eosinophilic material in the lumen of the tubules. For the diagnosis of onion poisoning, epidemiological data associated with clinical signs, such as pale mucous membranes with evidence of hemoglobin pigmentation (brown mucosa) and hemoglobinuria, should be taken into account. This article seems to be the first report of onion poisoning in cattle in Brazil.

The objective of this study was to evaluate the performance and intestinal health of nursery-phase piglets (22 to 64 days of age) fed a diet supplemented with organic acids and prebiotics or a diet supplemented with colistin. One hundred fifty weaned piglets 22 days old and with an initial weight of 5.56 kg were allotted to pens based on BW in a completely randomized block design to one of three treatment diets: T1, negative control; T2, colistin (10 mg/kg); and T3, mannan oligosaccharides + beta-glucans + ammonium formate, formic acid, ammonium propionate and acetic acid (1 kg/ton). The daily feed intake, daily weight gain, feed conversion, diarrhea score, cecal Lactobacillus, E. coli and fecal coliform content, and proinflammatory and anti-inflammatory cytokine concentrations were evaluated. The performance results considering the whole nursery phase showed a better (P < 0.05) feed conversion for T3 (1.593) and T2 (1.602) compared with T1 (1.679). The diarrhea score of T2 was lower than that of T1 (P < 0.05) and similar to that of T3 (P ≥ 0.05). The piglets that received T3 presented a greater concentration of proinflammatory cytokines at 36 days of age than T1, similar to T2 (p < 0.05), with the exception of TNFa, where T3 was superior to all groups, whereas at 57 days of age the piglets that received T2 presented a greater concentration of inflammatory cytokines. The piglets fed diets supplemented with a combination of mannan oligosaccharides, beta-glucans and acidifiers, representing an alternative to growth-promoting antibiotics, performed similarly to those fed colistin-supplemented diets.

This paper describes a spontaneous outbreak of toxic myopathy in finishing pigs due to the ingestion of feed with salinomycin associated with tiamulin and addresses the control methods performed during the outbreak to minimize economic losses resulting from the intoxication. A lot of 940 pigs fed feed containing 30 ppm salinomycin received 100 ppm tiamulin (via water) to control recurrent respiratory diseases on the farm. After ingesting tiamulin, some animals manifested clinical signs of motor incoordination, stiff gait, reluctance to move, muscle weakness and tremors, dyspnea, depression, and decubitus, remaining in “sitting-dog position” or with the abduction of the pelvic limbs, and rested on tip-toes when in a standing position. Two animals were euthanized for macro-and microscopic evaluation. The other sick animals received supportive anti-inflammatory treatment. The most relevant macroscopic finding observed during necropsy was the slight pallor of the pelvic limb muscles. The main histopathological findings consisted of multifocal areas of hyaline degeneration and marked necrosis of skeletal myofibers, with macrophage infiltrate associated with cell regeneration and skeletal fiber phagocytosis. These lesions were more intense in the longissimus dorsi, diaphragm, and masseter muscles. The definitive diagnosis was based on epidemiological aspects and clinical lesional conditions compatible with toxic myopathy secondary to ionophore intoxication.

This report describes the first case of idiopathic seasonal alopecia in a horse in Brazil. The disease is of unknown etiology, characterized by alopecic processes in the thoracic and lateral abdominal regions, in a bilaterally symmetrical way. An eight-year-old male grade horse was treated presenting hair loss in a bilaterally symmetrical manner in the arm and abdomen areas, without any other associated clinical signs. The areas with alopecia showed no pruritus, inflammation or scaling. On the epidermis, the histological evaluation presented irregular hyperplasia, hyperpigmentation, compact orthokeratosis, edema and an inflammatory infiltrate. The hair follicles were active and containing hair shaft. The case was monitored with photographic records for two consecutive years (2012 to 2014), in which the hair fall occurred at the end of autumn with spontaneous hair growth in the middle of the summer. The diagnosis was based on the history, histopathology and photographic follow-up performed. Although mentioned in the literature, this is the first clinical and pathological description of such disorder affecting an equine in Brazil.

This study identified the different forms of ingestion of Senecio brasiliensis in cattle, diagnosed by the Animal Pathology Laboratory at CAV-UDESC, in the state of Santa Catarina, Brazil. A retrospective evaluation from 1987 to 2016 showed that ingestion has occurred voluntarily due to the presence of the adult plant in native field and/or involuntary, due to the presence of the plant in hay and silage, provided in the trough together with other pastures or by contamination in grain residues. These different forms of ingestion demonstrate the importance of epidemiologic investigation in the diagnosis of seneciosis.

Knowledge about the causes of death in felines constitutes important information to owners, veterinarians, and researchers, aiming at reducing the number of deaths in this species. In order to determine the main causes of death or euthanasia in cats in the Santa Catarina plateau, data from 1995 to 2015 available in necropsy files of the Laboratory of Animal Pathology (LAPA) of the State University of Santa Catarina (UDESC) were collected and evaluated. In that period, 1,728 cats were necropsied, mainly males (46.12%) and adults (50.11%). The mean ages at death for kittens, adults, and elderly were 5.07 months, 3.9 years, and 13.9 years, respectively. Of the 1,728 necropsy reports assessed, the cause of death was identified in 1,184 (68.52%) cases. The main cause of death was associated with infectious diseases (15.8%), with prevalence of feline infectious peritonitis (29.76%), followed by neoplasms (11.98%) with lymphoma (44.93%) and leukemia (16.91%) as the most common, and traumas (11.81%) mainly caused by motor vehicle accidents. These results show the need for owner awareness, as well as establishment of prophylaxis and vaccination programs, aimed at reducing the number of deaths and thus increasing life expectancy in the feline population.