Ana Paula Carneiro
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Ana Paula Carneiro has a total of 13 co-authors. Here are the top co-authors by number of shared publications:
- Ana Carolina Silva Dias
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia 38405-320, Minas Gerais, Brazil
1 shared publication - Daniel Menezes-Souza
Colégio Técnico da UFMG, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, 31270-901, Brazil
1 shared publication - Eduardo A.F. Coelho
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia 38405-320, Minas Gerais, Brazil
1 shared publication - Eduardo Antonio Ferraz Coelho
Universidade Federal de Minas Gerais
1 shared publication - Fatima Ferreira
Department of Biosciences and Medical Biology University of Salzburg Salzburg Austria
1 shared publication - Fernanda Fonseca Ramos
Programa de Pós-Graduação em Ciências da Saúde, Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte 30.130-100, Minas Gerais, Brazil
1 shared publication
Latest Publications
- Open Access
- 12 April 2019
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 20 (8) , 1812
Leishmania infantum β-Tubulin Identified by Reverse Engineering Technology through Phage Display Applied as Theranostic Marker for Human Visceral Leishmaniasis
Two Leishmania infantum mimotopes (B10 and C01) identified by phage display showed to be antigenic and immunogenic for visceral (VL) and tegumentary (TL) leishmaniasis; however, their biological targets in the parasites have not been identified. The aim of the present study was to investigate the native antigens expressing both mimotopes, and to use them in distinct immunological assays. For this, a subtractive phage display technology was used, where a combinatorial library of single-chain variable fragments (scFv) was employed and the most reactive monoclonal antibodies for each target were captured, being the target antigens identified by mass spectrometry. Results in immunoblotting and immunoprecipitation assays showed that both monoclonal scFvs antibodies identified the β-tubulin protein as the target antigen in L. infantum. To validate these findings, the recombinant protein was cloned, purified and tested for the serodiagnosis of human leishmaniasis, and its immunogenicity was evaluated in PBMC derived from healthy subjects and treated or untreated VL patients. Results showed high diagnostic efficacy, as well as the development of a specific Th1 immune response in the cell cultures, since higher IFN-γ and lower IL-10 production was found.
- 12 April 2019
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 20 (8) , 1812
Open AccessLeishmania infantum β-Tubulin Identified by Reverse Engineering Technology through Phage Display Applied as Theranostic Marker for Human Visceral Leishmaniasis
Two Leishmania infantum mimotopes (B10 and C01) identified by phage display showed to be antigenic and immunogenic for visceral (VL) and tegumentary (TL) leishmaniasis; however, their biological targets in the parasites have not been identified. The aim of the present study was to investigate the native antigens expressing both mimotopes, and to use them in distinct immunological assays. For this, a subtractive phage display technology was used, where a combinatorial library of single-chain variable fragments (scFv) was employed and the most reactive monoclonal antibodies for each target were captured, being the target antigens identified by mass spectrometry. Results in immunoblotting and immunoprecipitation assays showed that both monoclonal scFvs antibodies identified the β-tubulin protein as the target antigen in L. infantum. To validate these findings, the recombinant protein was cloned, purified and tested for the serodiagnosis of human leishmaniasis, and its immunogenicity was evaluated in PBMC derived from healthy subjects and treated or untreated VL patients. Results showed high diagnostic efficacy, as well as the development of a specific Th1 immune response in the cell cultures, since higher IFN-γ and lower IL-10 production was found.