Beibei Luo

Background: As a traditional Chinese exercise, Wushu has been shown to effectively promote balance and postural stability in various populations. Preschoolers’ capacity for balance control, including static, dynamic, and dual-task balance, is linked to the later development of stability skills in adulthood. However, studies of Wushu intervention focusing on balance ability and the related physical fitness in preschool children are limited. Objectives: This study investigated the effects of a 10-week Wushu program on static, dynamic dual-task balance and physical fitness in children 5–6 years old. Methods: Seventy-three participants were randomly divided into an intervention (INT, n = 39) and a control (CON, n = 34) group. The INT group participated in a 10-week Wushu program that included three 30 min sessions per week, while the CON group engaged in unstructured free play with purposely designed materials. The three key primary outcomes were dominant-leg stance for static balance, the balance beam walk for dynamic balance, and the center of pressure (CoP) path length obtained via a force platform during dual-task balance testing, in which the participants were instructed to count numbers backward. The five derived primary outcomes were non-dominant-leg stance, CoP ML path length, CoP AP path length, CoP sway velocity, and CoP sway area. Secondary outcomes were physical fitness indicators, including sit and reach, grip strength, standing long jump (SLJ), countermovement jump (CMJ), 15 m zigzag run, double-leg continuous jump, height and body weight. The analysis of the outcomes was conducted using analysis of covariance (ANCOVA) and Pearson correlation. Results: At baseline, the CON and INT groups did not differ significantly. The INT demonstrated significant enhancements in comparison with CON in the dominant and non-dominant-leg stance (p = 0.017 and p = 0.005, respectively), the balance beam walk, SLJ, 15 m zigzag run and double-leg continuous jump (all p < 0.05), along with the mediolateral CoP path length (p = 0.012). A strong correlation was found between the balance beam walk and the double-leg continuous jump (r = 0.55, p = 0.001), and between the balance beam walk and the 15 m zigzag run (r = 0.43, p = 0.015). Conclusions: The present study demonstrates that a 10-week Wushu program significantly enhances preschool children’s static balance, dynamic balance, and mediolateral postural control during dual-task condition. The improvements in dynamic balance are correlated with lower-limb coordination and jumping agility.

Background: Wushu, a traditional Chinese exercise, has been demonstrated to be effective in promoting lower-limb strength in children. However, studies comparing the effects of different intervention durations on preschool children remain limited. Objectives: The present study examined the short- and long-term effects of Wushu exercise programs on lower-limb explosive power in preschool children aged 5–6 years. Methods: This study was conducted across two experiments, with separate cohorts of children. The children were randomly assigned to either an intervention (INT) or a control (CON) group based on their Kindergarten classes. In Experiment 1, the INT-1 group (n = 55) completed a 4-week ‘Twelve Zodiac’ Wushu exercise program, which comprised three 30-minute sessions per week, while the CON-1 group (n = 49) participated in construction and carrying-based unstructured free play, which was designed to provide a comparable amount of moderate-to-vigorous physical activity. In Experiment 2, the INT-2 group (n = 57) undertook a 10-week Wushu program, and the CON-2 group (n = 38) engaged in similar activities as CON-1 for a 10-week period. The standing long jump (SLJ) was the primary outcome measure in both experiments. Secondary outcomes included the double-leg continuous jump, 15 m zigzag run, grip strength, sit-and-reach, and anthropometric measurements. In Experiment 2, countermovement jump (CMJ) and squat jump (SJ) heights were also measured using a force plate as additional secondary outcomes. A linear mixed-effects model (LMM) was used to analyze the data. Results: At baseline, no significant outcome measures were observed between CON-1 and INT-1, nor between CON-2 and INT-2. In Experiment 1, SLJ exhibited a significant enhancement in INT-1 in comparison to CON-1 (p = 0.007). The INT-2 in Experiment 2 showed significant improvements compared with CON-2 in the SLJ (p = 0.048), double-leg continuous jump (p = 0.005), and 15 m zigzag run (p = 0.043). A strong correlation was observed between SLJ and 15 m zigzag run time (r = −0.53, p < 0.001), and between double-leg continuous jump time and 15 m zigzag run time (r = 0.56, p < 0.001). Conclusions: The findings of this study indicate that 4-week and 10-week Wushu exercise programs enhance explosive power in the lower limbs of children aged 5–6 years. The 10-week Wushu program improves lower limb coordination and jumping agility. These task-specific adaptations support the value of Wushu interventions for fostering comprehensive lower-limb motor competence in preschoolers.

Background: The process of muscle protein synthesis (MPS) plays a pivotal role in the enhancement of muscle function. Following a bout of exercise, the rate of MPS experiences an elevation for a brief period, known as the “anabolic window.” Despite whey protein supplementation has been demonstrated to augment the post-exercise anabolic window, the optimal timing and dosage remain controversial. Therefore, the present systematic review and meta-analysis were conducted to evaluate the effects of whey protein supplementation on post-exercise MPS and its protein kinase B (AKT)/mammalian target of the rapamycin (mTOR) pathway in healthy adults. Methods: Following PRISMA guidelines, this review included 21 RCTs, with 15 studies subjected to meta-analysis and 6 studies to qualitative analysis. Eligible studies examined myofibrillar fractional synthetic rate (FSR) or the AKT/mTOR pathway-related protein phosphorylation levels in muscle biopsy samples. Results: The combination of whey protein supplementation and exercise has been shown to significantly enhance FSR (Hedge’s g = 1.24, 95% CI: 0.71–1.77; p < 0.001), with increases ranging from 1.3 to 1.6 folds when consumed immediately after exercise and up to 2.5 folds when given 45 min prior to multiple-set resistance exercise. A dose-dependent increase in FSR was observed in response to whey protein supplementation, ranging from 10 to 60 g. In comparison to the placebo group, whey protein supplementation enhanced the phosphorylation levels of AKT, mTOR, eukaryotic translation initiation factor 4E-binding protein-1 (4E-BP1), 70 kDa ribosomal protein S6 kinase (p70S6K), and ribosomal protein S6 (rpS6) at 1–2 h post-exercise. Phosphorylation levels of p70S6K and rpS6 decreased 4–5 h after exercise. Conclusions: The combination of whey protein supplementation and exercise improves MPS in a time- and dose-dependent manner. Consumption of 20–40g of whey protein before multiple sets of resistance exercise may enhance myofibrillar FSR and activate the AKT/mTOR pathway, thereby augmenting MPS and extending the anabolic window.

Background: Mental health issues in preschool children are a significant public health concern with long-term developmental implications. Understanding how reallocations of time among different 24-hour movement behaviors-moderate-to-vigorous physical activity (MVPA), light physical activity (LPA), sedentary behavior (SED), and sleep (SLP)-affect various dimensions of mental health is essential for designing effective interventions. Methods: In this cross-sectional study, 828 Chinese preschool children aged 3 to 6 years were assessed. Physical activity and sleep were objectively measured using accelerometers to capture MVPA, LPA, SED, and SLP. Mental health was evaluated using the Strengths and Difficulties Questionnaire (SDQ), assessing Total Difficulties, Internalizing Problems, Externalizing Problems, and Prosocial Behavior. Compositional data analysis was conducted using isometric log-ratio (ilr) transformation, followed by multivariate linear regression to assess associations. Additionally, isotemporal substitution modeling and dose-response analysis were applied to examine the effects of reallocating time between behaviors on mental health outcomes, adjusting for age, gender, BMI, parental education, and parental attitudes toward physical activity. Results: Increased MVPA was significantly associated with lower Total Difficulties (ß_MVPA= − 1.587; P < 0.001) and Internalizing Problems (ß_MVPA= − 0.663; P = 0.017). Increased SED was associated with higher Total Difficulties (ß_SED= 1.512; P < 0.05), while increased SLP was linked to improved Externalizing Problems (ß_LPA= − 1.792; P = 0.008). Reallocating as little as 1 min from LPA or SED to MVPA or SLP significantly reduced Total Difficulties and Internalizing Problems (P < 0.05). Conversely, replacing SED or SLP with LPA increased Externalizing Problems, particularly Conduct Problems and Hyperactivity/Inattention (P < 0.05). A critical threshold at 30 min was identified, beyond which the effects on mental health outcomes became more pronounced. Conclusions: Reallocating time from LPA or SED to MVPA or SLP significantly improves Total Difficulties and Internalizing Problems in preschool children, even with brief substitutions. However, increasing LPA at the expense of SED or SLP exacerbates Externalizing Problems, especially Conduct Problems and Hyperactivity/Inattention. Interventions should promote MVPA and adequate sleep while considering the critical 30-minute threshold where effects become more pronounced to enhance emotional and behavioral well-being.

Organoid, formed from organ-specific cells, is a group of self-renewal and self-organizing cells growing in a 3-dimensional structure. With the recent progress on microenvironment regulation, stem cell differentiation and organ development, organoids have been constructed and used as promising tools for a wide range of multidisciplinary biomedical applications. Exercise disrupts the internal environment homeostasis, which brings a series of physiological alterations to the digestive system. The current animal or human models are necessary, but not sufficient to monitor the fluctuating microenvironment of gastrointestinal epithelial cells or hepatocytes during exercise. This review described the construction and application of digestive system organoids, as well as the effect of exercise on the microenvironment of intestinal epithelial cells and hepatocytes. The perspective applications of digestive system organoids in exercise physiology were also stated. Using organoid technologies, the possible mechanisms of the exercise-induced dynamic physiological changes would be explored in a new dimension.

A novel synthesis approach for the construction of 2-trifluoroethyl-substituted benzofurans under copper-catalyzed conditions has been developed. The reaction of salicylaldehyde p-tosylhydrazones with 2-bromo-3,3,3-trifluoropropene proceeded through a sequential process to afford products in moderate to excellent yields (up to 93%). This method is also applicable to the formation of 2-trifluoroethylated indoles.

This is a non-final version of an article published in final form in the journal Medicine and Science in Sports and Exercise, 47: 5(S) and can be found at: http://dx.doi.org/10.1249/01.mss.0000466118.29837.6

This paper is Part A of a two paper series seeking a systematical investigation of the feasibility of separating binary solvent mixtures with solvent resistant nanofiltration (SRNF) membranes.

This study determined if 6-weeks vitamin D₂ supplementation (vitD₂, 3800 IU/day) had an influence on muscle function, eccentric exercise-induced muscle damage (EIMD), and delayed onset of muscle soreness (DOMS) in National Association for Stock Car Auto Racing (NASCAR) NASCAR pit crew athletes. Subjects were randomized to vitD₂ (n = 13) and placebo (n = 15), and ingested supplements (double-blind) for six weeks. Blood samples were collected and muscle function tests conducted pre- and post-study (leg-back and hand grip dynamometer strength tests, body weight bench press to exhaustion, vertical jump, 30-s Wingate test). Post-study, subjects engaged in 90 min eccentric-based exercise, with blood samples and DOMS ratings obtained immediately after and 1- and 2-days post-exercise. Six weeks vitD₂ increased serum 25(OH)D₂ 456% and decreased 25(OH)D₃ 21% versus placebo (p < 0.001, p = 0.036, respectively), with no influence on muscle function test scores. The post-study eccentric exercise bout induced EIMD and DOMS, with higher muscle damage biomarkers measured in vitD₂ compared to placebo (myoglobin 252%, 122% increase, respectively, p = 0.001; creatine phosphokinase 24 h post-exercise, 169%, 32%, p < 0.001), with no differences for DOMS. In summary, 6-weeks vitD₂ (3800 IU/day) significantly increased 25(OH)D₂ and decreased 25(OH)D₃, had no effect on muscle function tests, and amplified muscle damage markers in NASCAR pit crew athletes following eccentric exercise.