Sandra Cecilia Ramírez López

Lipid droplets (LDs) and the endoplasmic reticulum (ER) are dynamic organelles central to cellular metabolism, signaling, and stress response. Despite their biological relevance and close interplay, tools to selectively track their dynamic behavior remain limited. Here, the design, synthesis, and characterization of a new class of fluorescent probes obtained through green multicomponent synthetic strategies are reported. These previously unexplored fluorophore scaffolds display unexpected and differential subcellular localization, selectively targeting either LDs or the ER with high photostability. Mechanistic studies suggest that such selectivity arises from a synergistic interplay between partition coefficient, conformational geometry, and strategically positioned targeting moieties. These features enable the probes to adapt to distinct microenvironments within cells, supporting real‐time visualization of both LD and ER dynamics. These findings provide versatile sensing tools and novel insights into organelle‐targeting principles, establishing an eco‐conscious route to next‐generation fluorescent sensors.

A series of three 2,5-Diketopiperazine (DKPs) were synthesized via one-pot process through the post- isocyanide-based multicomponent reactions (IMCR)-transformation strategy. This strategy emphasizes the role of orthogonal bifunctional reagents in the IMCR process to increase their synthetic potential, allowing us accessing a synthetic platform from which it is possible to obtain privileged heterocyclic peptidomimetics via lactamization reaction.

The synthesis of novel and complex molecules of tetrakis-tetrazole was done via a Ugi-azide repetitive reaction from easily accessible starting materials in good yields. The use of orthogonal bifunctional reagents in isocyanide-based multicomponent reactions (IMCR) allowed the synthesis of structurally complex molecules in one pot manner. The molecules herein synthesized could have applications such as use as chelating agents and organocatalysts.

A serie of linked bis-heterocyclic privileged peptidomimetic scaffolds are synthesized via a green one-pot strategy based IMCR/post-transformation/tandem. This is the first one-pot synthesis of a 2,5-diketopiperazine linked to a 1,4-disubstituted 1,2,3-triazole. The sequence used in the present work Ugi-4CR/lactamization/click is facile, mild, atom-economical, and green.

A series of six new alkyne-2-chloroquinolines were synthesized in moderate yields (40%–65%) via the Passerini three-component reaction (P-3CR) under mild green conditions. The P-3CR takes place when a carboxylic acid, an oxo compound (aldehyde or ketone), and an isocyanide react to give α-acyloxy carboxamides. Recently it has been reported that small molecules containing alkynes promote interactions with different proteins in cells facilitating the detection or identification of protein targets.

A series of six fused bis-heterocycles having imidazo[1,2-a]pyridine bound with quinoline were synthesized by microwave-assisted Groebke-Blackburn-Bienaymé reaction (GBBR) under green catalysis. The GBB products are privileged scaffolds and their synthesis is of great interest in synthetic and medicinal chemistry.

An efficient synthesis of alkyne-3-tetrazolyl-tetrazolo[1,5-a] quinolones via a one-pot isocyanide-based multicomponent reaction (IMCR) process: I-MCR Ugi-azide/S_NAr/ring-chain azido tautomerization was performed under eco-friendly conditions. We report the one-pot synthesis of tris-heterocycles containing a tetrazolo[1,5-a] quinoline connected to a 1,5-disubstituted-tetrazole (1,5-DS-T). The synthesis of these compounds is of great interest in synthetic and medicinal chemistry because these heterocycles are considered privileged scaffolds and their preparation and evaluation may lead to the discovery of novel bioactive molecules.

A solvent-free and catalyst-free synthesis of fused bis-heterocycles containing imidazo[1,2-a]pyridine and tetrazolo[1,5-a]quinoline frameworks is reported via a one-pot process. This Groebke–Blackburn–Bienaymé reaction (GBBR)/S_NAr/ring-chain azido tautomerization cascade proceeds under eco-friendly conditions. The tetrazolo[1,5-a]quinoline and imidazo[1,2-a]pyridine scaffolds are present in various compounds with interesting pharmacological properties and could lead to the discovery of novel bioactive molecules.