Nurullah Keklikoglu

Aim: Although there are many materials that can be used for retrograde filling in surgical endodontics, none of them can be regarded as an ideal material yet. The purpose of this study was to compare the surface topography of three different root-end filling materials.Methods: 36 extracted single rooted human incisor teeth were cleaned and decoronated to standardized 10 mm root lengths. The root segments were prepared and 2 mm apical resection were performed. The samples were randomly separeted to three groups (Group A: Ca(OH)2, Group B: MTA Angelus, Group C: ProRoot MTA), each comprised of 12 roots. Materials were placed as 2 mm apical barriers and obturated with guttapercha and AH-Plus sealer. Each group dimidiated two subgroups (A1,A2,B1,B2,C1,C2). Groups A1,B1,C1 were stored in normal saline (NS), groups A2,B2,C2 were stored in neutral phosphate buffer saline (NPBS) solution and samples were incubated at 370C for 2 weeks. Stereomicroscope (32X) was used to photograph the root-end filling.Results: All specimens demonstrated white crystals formation and sediment over the root-end filling materials and on the superficial border of the root-end cavities’ wall as a white plague. A2,B2,C2 samples have more crystal sediment on root-end fillings than samples A1,B1,C1. Dissolution and corrosion were observed in groups A1, A2.Conclusions: The results of this study revealed that calcium hydroxide is more resorbable than MTA Angelus and ProRoot MTA. The crystals formation and precipitation were observed in neutral phosphate buffer saline solution was more than normal saline solution for all groups as a hydroxiapatite crystals.

The histological processing of teeth is highly complicated because of containing both mineralized hard tissues and soft tissues. Depending on the type of decalcification agents used in processing, mild-to-severe deterioration in the tissue structure and inadequacies on clear staining of details by the histological stain may be observed. This study aims to compare the histological staining differences in the preparations from decalcified and undecalcified tooth roots by three different embedding materials and techniques. Following extraction, human single-rooted teeth crowns were cut off and roots were placed in 10% buffered neutral formalin. After fixation, roots were divided into two groups. One part of samples was decalcified in formic acid solution and the other was remained undecalcified. Decalcified roots were embedded in paraffin and glycol methacrylate (GMA)-based resin and undecalcified roots were embedded in methyl methacrylate (MMA)-based resin. Sections from all groups were stained with hematoxylin and eosin. The groups were compared in terms of general staining, brightness, density, density of the base stain, general morphology of cells, nuclear/cytoplasmic contrast, distinguish ability of pulp, odontoblast layer, predentin and dentin, preservation and traceability of dentinal tubule. In the preparations which were embedded into the MMA-based embedding material, an output lower than the paraffin group buthigher than the GMA-embedded group was provided. As a result, the best histological detail was obtained from the decalcified, paraffin-embedded sections. Abstract: The histological processing of teeth is highly complicated because of containing both mineralized hard tissues and soft tissues. Depending on the type of decalcification agents used in processing, mild-to-severe deterioration in the tissue structure and inadequacies on clear staining of details by the histological stain may be observed. This study aims to compare the histological staining differences in the preparations from decalcified and undecalcified tooth roots by three different embedding materials and techniques. Following extraction, human single-rooted teeth crowns were cut off and roots were placed in 10% buffered neutral formalin. After fixation, roots were divided into two groups. One part of samples was decalcified in formic acid solution and the other was remained undecalcified. Decalcified roots were embedded in paraffin and glycol methacrylate (GMA)-based resin and undecalcified roots were embedded in methyl methacrylate (MMA)-based resin. Sections from all groups were stained with hematoxylin and eosin. The groups were compared in terms of general staining, brightness, density, density of the base stain, general morphology of cells, nuclear/cytoplasmic contrast, distinguish ability of pulp, odontoblast layer, predentin and dentin, preservation and traceability of dentinal tubule. In the preparations which were embedded into the MMA-based embedding material, an output lower than the paraffin group buthigher than the GMA-embedded group was provided. As a result, the best histological detail was obtained from the decalcified, paraffin-embedded sections.