Chen Sun

The global fish industry is an essential contributor to food security and the economy; however, it produces considerable quantities of fish waste. Depending on species and processing methods, 20–80% of fish biomass is discarded as waste, amounting to almost 64 million tons annually. Conventional disposal methods such as landfilling, incineration, and wastewater discharge cause environmental pollution and greenhouse gas emissions. Therefore, for sustainable development, it is important to valorize fish waste into renewable energy and value-added products. Hence, this review aims to encourage the technical development of fish waste into bioenergy and bioresources by thoroughly studying key technologies for converting it into biogas, biodiesel, fertilizer, animal feed, and biochar. Although several studies have explored these technologies, most remain disjointed and lack a systematic assessment. Therefore, this work attempted to critically evaluate the technological principles, advantages, limitations, and optimization strategies of current valorization approaches. Furthermore, several recommendations have been proposed, including conducting pilot-scale trials, producing higher value-added products within a biorefinery system. Overall, this review provides a holistic outlook on the energy and resource conversion of fish waste, supporting sustainable waste management.

In this work, the corn straw (CS) with concentrations of 3%, 6%, and 9% (w/v) were pretreated by rumen fluid (RF) and then used for batched mesophilic biogas production. The results showed that after a 6-day pretreatment, volatile fatty acid (VFAs) production of 3.78, 8.27, and 10.4 g/L could be found in 3%, 6%, and 9%, respectively. When concerning with biogas production, the highest accumulative methane production of 149.1 mL CH₄/g volatile solid was achieved by 6% pretreated CS, which was 22% and 45% higher than 3% and 9%, respectively. Also, it was 3.6 times higher than the same concentration of unpretreated CS. The results of the microbial community structure analysis revealed that the 6% CS pretreatment not only maintained a microbial community with the highest richness and diversity, but also exhibited the highest relative abundance of Firmicutes (45%) and Euryarchaeota (3.9%). This high abundance was conducive to its elevated production of VFAs and methane. These findings provide scientific reference for the utilization of CS and support the development of agricultural waste resource utilization and environmental protection.

In this work, the corn stover (CS) with concentration of 3%, 6% and 9% (w/v) were pretreated by rumen fluid and then used for batched mesophilic biogas production. The results showed that after a 6-day pretreatment, VFAs production of 3.78, 8.27, and 10.4 g/L could be found in 3%, 6% and 9%, respectively. When concerning with biogas production, the highest accumulative methane production of 149.1 mL CH₄/g VS was achieved by 6% pretreated CS, which was 22% and 45% higher than 3% and 9%, respectively. Also, it was 3.6 times higher than the same concentration of unpretreated CS. The results of microbial community structure revealed highest relative abundance of Firmicutes (45%) and Euryarchaeota (3.9%) in 6% CS, which was beneficial to its high VFAs and methane production. These findings provide scientific reference for the utilization of CS and support the development of agricultural waste resource utilization and environmental protection.

In order to improve the performance of methane production from agro-waste, a batched four-stage–two-phase anaerobic digestion (4S2P-AD) system was designed to combine the advantages of both anaerobic co-digestion (co-AD) and two-phase AD. The initial separation of two phases was performed using rice straw (RS) as a feedstock in acidogenic phase and cow manure (CM) in methanogenic phase at low inoculum/substrate (I/S) ratios of 0.5 and 0.2 and a high organic loading of 60 g volatile solid (VS)/L. The periodic round-trip reflux of leachate during the 4S2P-AD process facilitated re-inoculation throughout the four stages. The results showed that this round-trip reflux also dispersed toxic ammonia, balanced the carbon/nitrogen ratio, unified the microbial community structure, and led to the selection of Methanosarcina (relative abundance > 80%) as the dominant methanogens. With the abilities to overcome volatile fatty acid accumulation, shorten lag times, improve biodegradability, and foster synergistic effects, it was verified that the 4S2P-AD process can maintain efficient and stable methanogenesis from high-solid lignocellulosic feedstock. The averaged methane production throughout the four stages of 4S2P-AD was 234 mL/g VS. This result is 96% higher than the averaged methane production obtained from the four one-step AD groups using mono-feedstock, and 91% higher than that obtained using co-feedstock. This study provides a scientific reference for the development of new processes of bio-methane production from agro-waste with a high fermentation capacity and stability in the future.

The pretreatment effects and synergistic effects of anaerobic co-digestion of pretreated corn stover (CS) with chicken manure (CM) were studied. Results showed that the NaOH-H₂O₂ pretreatment effect on CS was better than urea pretreatment in terms of anaerobic digestion promotion. The highest cumulative methane yield of 332.7 mL/g VS added was obtained from the CS (NaOH-H₂O₂ pretreated)/CM ratio of 1:3, and the highest cumulative methane yield of 319.7 mL/g VS added was obtained from the CS (urea pretreated)/CM ratio of 1:2. Synergistic effects were found in CS (NaOH-H₂O₂ pretreated)/CM ratios of 2:1, 1:2, 1:3 and CS (urea pretreated)/CM ratios of 1:1, 1:2. Synergistic effect was not found at CS (unpretreated)/CM ratios of 1:2 and 1:3. Pretreatment of CS can produce synergistic effect on anaerobic co-digestion and increase cumulative methane yield by 6.54–24.65%. Among the four kinetic models, modified Gompertz model was best fitted in describing the methane production during anaerobic co-digestion (R² = 0.9845–0.9988).

The Yi River, the second longest river in Shandong Province, China, flows through Linyi City and is fed by three tributary rivers, Beng River, Liuqing River, and Su River in the northeastern part of the city. In this study, we determined the concentrations of five heavy metals (Cr, Ni, Cu, Zn, and Pb) in water, sediment, and aquatic macrophyte samples collected from the junction of the four rivers and evaluated the potential ecological risk of heavy metal pollution. Most of the heavy metals in water were in low concentrations with the water quality index (WQI) below 1, suggesting low metal pollution. The sediments showed low heavy metal concentrations, suggesting a low ecological risk based on the potential ecological risk index (RI) and the geo-accumulation index (I_geo). The aquatic plant species Potamogeton crispus accumulated considerable amounts of heavy metals, which were closely related to the metal concentrations of the sediment. The plant species Salvinia natans also showed an excellent metal accumulation capability. Based on our results, the junction of the four rivers is only slightly polluted in terms of heavy metals, and the plant species P. crispus is a suitable bioindicator for sediment heavy metal pollution.