Lexia N. Wilson
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Lexia N. Wilson has a total of 13 co-authors. Here are the top co-authors by number of shared publications:
- Bailey E. Lucas
Department of Molecular Genetics, The Ohio State University, Marion, OH 43302, USA
1 shared publication - Hannah M. Hylton
Biology Program, The Ohio State University, Marion, OH 43302, USA
1 shared publication - Jacob M. Kroh
Department of Molecular Genetics, The Ohio State University, Marion, OH 43302, USA
1 shared publication - Jian-Qiu Wu
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210
1 shared publication - Kyle G. Hickman
Department of Molecular Genetics, The Ohio State University, Marion, OH 43302, USA
1 shared publication - Matthew T. McPherson
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43215, USA
1 shared publication
Latest Publications
- Open Access
- 26 August 2019
- Published by MDPI AG in Methods and Protocols
- Vol. 2 (3) , 74
An Assay to Study Intra-Chromosomal Deletions in Yeast
An accurate DNA damage response pathway is critical for the repair of DNA double-strand breaks. Repair may occur by homologous recombination, of which many different sub-pathways have been identified. Some recombination pathways are conservative, meaning that the chromosome sequences are preserved, and others are non-conservative, leading to some alteration of the DNA sequence. We describe an in vivo genetic assay to study non-conservative intra-chromosomal deletions at regions of non-tandem direct repeats in Schizosaccharomyces pombe. This assay can be used to study both spontaneous breaks arising during DNA replication and induced double-strand breaks created with the S. cerevisiae homothallic endonuclease (HO). The preliminary genetic validation of this assay shows that spontaneous breaks require rad52+ but not rad51+, while induced breaks require both genes, in agreement with previous studies. This assay will be useful in the field of DNA damage repair for studying mechanisms of intra-chromosomal deletions.
- 26 August 2019
- Published by MDPI AG in Methods and Protocols
- Vol. 2 (3) , 74
Open AccessAn Assay to Study Intra-Chromosomal Deletions in Yeast
An accurate DNA damage response pathway is critical for the repair of DNA double-strand breaks. Repair may occur by homologous recombination, of which many different sub-pathways have been identified. Some recombination pathways are conservative, meaning that the chromosome sequences are preserved, and others are non-conservative, leading to some alteration of the DNA sequence. We describe an in vivo genetic assay to study non-conservative intra-chromosomal deletions at regions of non-tandem direct repeats in Schizosaccharomyces pombe. This assay can be used to study both spontaneous breaks arising during DNA replication and induced double-strand breaks created with the S. cerevisiae homothallic endonuclease (HO). The preliminary genetic validation of this assay shows that spontaneous breaks require rad52+ but not rad51+, while induced breaks require both genes, in agreement with previous studies. This assay will be useful in the field of DNA damage repair for studying mechanisms of intra-chromosomal deletions.