Andrea Roztocilova

The aim of the study was to determine the calcium requirements in preparation period of the sexual mature and egg-laying and evaluate development of calcium retention in the first laying period of the hybrid combination Bovans Brown hens. The ...

The main objective of this study was to verify the safety of mealworm meal in the feed of laying hens from 17 to 42 weeks of age. Therefore, the feed mixtures were tested in terms of microbiological stability, fungal and mycotoxin content and selected parameters of hens’ intestinal morphology and physiology were monitored. The experiment was carried out with 30 Lohmann Brown Classic hens. Hens were divided by body mass into three equal groups with 10 replicates per treatment. The two experimental groups received feed mixtures containing 2% and 5% yellow mealworm (Tenebrio molitor L.) meal. The third group was a control group which had 0% of mealworm meal in the diet. Diets with 2% and 5% of mealworm meals did not affect the length of villi and microbiome of the caecum. The highest digesta viscosity from the ileum was found in the group with 5% mealworm, which may indicate a slower passage of the digesta through the digestive tract. Based on our results, it may be concluded that the proportion of mealworm meals does not deteriorate the quality of feeds. Mealworm meal does not negatively affect microbial stability in experimental feeds. Therefore, it can be recommended the two and (or) five percent of mealworm meal inclusion in hen’s diet.

The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of Escherichia coli or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 h following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 h after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-h following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, the scientific journal of the Mendel University in Brno, Czech Republic