Manishi Pallavi
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Manishi Pallavi has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Jagannathan Sankar
Department of Mechanical Engineering, North Carolina A&T State University, Greensboro, NC 27411, USA
2 shared publications - Jenora T. Waterman
Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
2 shared publications - Yeoheung Yun
FIT BEST Laboratory, Department of Chemical, Biological and Bio Engineering, NERVE Center, North Carolina Agricultural and Technical State University, Greensboro, USA
1 shared publication - Yeoheung Yun
Engineering Research Center for Revolutionized Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
1 shared publication - Youngmi Koo
Engineering Research Center for Revolutionized Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
1 shared publication - Youngmi Koo
Engineering Research Center for Revolutionized Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
1 shared publication
Latest Publications
- Open Access
- 20 March 2020
- Published by MDPI AG in Applied Sciences
- Vol. 10 (6) , 2114
Assessment of Cytotoxicity of Magnesium Oxide and Magnesium Hydroxide Nanoparticles using the Electric Cell-Substrate Impedance Sensing
Magnesium (Mg)-based alloys have the potential for bone repair due to their properties of biodegradation, biocompatibility, and structural stability, which can eliminate the requirement for a second surgery for the removal of the implant. Nevertheless, uncontrolled degradation rate and possible cytotoxicity of the corrosion products at the implant sites are known current challenges for clinical applications. In this study, we assessed in vitro cytotoxicity of different concentrations (0 to 50 mM) of possible corrosion products in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg(OH)2) nanoparticles (NPs) in human fetal osteoblast (hFOB) 1.19 cells. We measured cell proliferation, adhesion, migration, and cytotoxicity using a real-time, label-free, non-invasive electric cell-substrate impedance sensing (ECIS) system. Our results suggest that 1 mM concentrations of MgO/Mg(OH)2 NPs are tolerable in hFOB 1.19 cells. Based on our findings, we propose the development of innovative biodegradable Mg-based alloys for further in vivo animal testing and clinical trials in orthopedics.
- 20 March 2020
- Published by MDPI AG in Applied Sciences
- Vol. 10 (6) , 2114
Open AccessAssessment of Cytotoxicity of Magnesium Oxide and Magnesium Hydroxide Nanoparticles using the Electric Cell-Substrate Impedance Sensing
Magnesium (Mg)-based alloys have the potential for bone repair due to their properties of biodegradation, biocompatibility, and structural stability, which can eliminate the requirement for a second surgery for the removal of the implant. Nevertheless, uncontrolled degradation rate and possible cytotoxicity of the corrosion products at the implant sites are known current challenges for clinical applications. In this study, we assessed in vitro cytotoxicity of different concentrations (0 to 50 mM) of possible corrosion products in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg(OH)2) nanoparticles (NPs) in human fetal osteoblast (hFOB) 1.19 cells. We measured cell proliferation, adhesion, migration, and cytotoxicity using a real-time, label-free, non-invasive electric cell-substrate impedance sensing (ECIS) system. Our results suggest that 1 mM concentrations of MgO/Mg(OH)2 NPs are tolerable in hFOB 1.19 cells. Based on our findings, we propose the development of innovative biodegradable Mg-based alloys for further in vivo animal testing and clinical trials in orthopedics.
- Open Access
- 14 October 2019
- Published by MDPI AG in Applied Sciences
- Vol. 9 (20) , 4304
In Vitro Cytotoxicity of Possible Corrosion Products from Mg-Based Biodegradable Metals: Magnesium Oxide and Magnesium Hydroxide Nanoparticles
Biodegradable magnesium (Mg) alloys have potential applications in orthopedic implants due to their mechanical and osseointegration properties. However, the surface characteristics, biocompatibility, and toxicity of the released corrosion products in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg(OH)2) nanoparticles (NPs) at the junction of implants and in the surrounding tissue are not completely understood. Here, we investigated in vitro cytotoxicity and morphological changes in human fetal osteoblast (hFOB) 1.19 cells in response to various concentrations (1 mM, 5 mM, 10 mM, and 50 mM) of MgO/Mg(OH)2 NPs by live/dead assay and scanning electron microscopy (SEM). In this study, we performed a surface characterization of MgO/Mg(OH)2 NPs to evaluate the size of the NPs. Further, an immersion test was performed in Dulbecco’s Modified Eagle’s Medium (DMEM) with randomly selected various concentrations (1 mM, 5 mM, 10 mM, 50 mM, and 100 mM) of MgO/Mg(OH)2 NPs to understand the degradation behavior of the NPs, and the change in the pH values from days 1 to 7 was measured. After conducting an immersion test for seven days, the highest concentration (100 mM) of MgO/Mg(OH)2 NPs was selected to study the element depositions on nanoparticles through scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM–EDX) mapping. The results from this in vitro cytotoxicity study suggest that less than or equal to 5-mM concentrations of MgO/Mg(OH)2 NPs are tolerable concentrations for hFOB 1.19 cells. This study provides a foundational knowledge of MgO/Mg(OH)2 NP cytotoxicity in hFOB 1.19 cells that can help to develop future sustainable biodegradable magnesium-based alloys for orthopedic applications.
- 14 October 2019
- Published by MDPI AG in Applied Sciences
- Vol. 9 (20) , 4304
Open AccessIn Vitro Cytotoxicity of Possible Corrosion Products from Mg-Based Biodegradable Metals: Magnesium Oxide and Magnesium Hydroxide Nanoparticles
Biodegradable magnesium (Mg) alloys have potential applications in orthopedic implants due to their mechanical and osseointegration properties. However, the surface characteristics, biocompatibility, and toxicity of the released corrosion products in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg(OH)2) nanoparticles (NPs) at the junction of implants and in the surrounding tissue are not completely understood. Here, we investigated in vitro cytotoxicity and morphological changes in human fetal osteoblast (hFOB) 1.19 cells in response to various concentrations (1 mM, 5 mM, 10 mM, and 50 mM) of MgO/Mg(OH)2 NPs by live/dead assay and scanning electron microscopy (SEM). In this study, we performed a surface characterization of MgO/Mg(OH)2 NPs to evaluate the size of the NPs. Further, an immersion test was performed in Dulbecco’s Modified Eagle’s Medium (DMEM) with randomly selected various concentrations (1 mM, 5 mM, 10 mM, 50 mM, and 100 mM) of MgO/Mg(OH)2 NPs to understand the degradation behavior of the NPs, and the change in the pH values from days 1 to 7 was measured. After conducting an immersion test for seven days, the highest concentration (100 mM) of MgO/Mg(OH)2 NPs was selected to study the element depositions on nanoparticles through scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM–EDX) mapping. The results from this in vitro cytotoxicity study suggest that less than or equal to 5-mM concentrations of MgO/Mg(OH)2 NPs are tolerable concentrations for hFOB 1.19 cells. This study provides a foundational knowledge of MgO/Mg(OH)2 NP cytotoxicity in hFOB 1.19 cells that can help to develop future sustainable biodegradable magnesium-based alloys for orthopedic applications.