Joe P Hsiao
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Joe P Hsiao has a total of 12 co-authors. Here are the top co-authors by number of shared publications:
- Brett M. Condon
Department of Biochemistry
1 shared publication - Chad Michael Hobson
Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA 20147, USA
1 shared publication - Edward Timothy O’brien
Department of Physics and Astronomy, The University of North Carolina, Chapel Hill, NC 27514, USA
1 shared publication - Evan Nelsen
The University of North Carolina at Chapel Hill
1 shared publication - Klaus Michael Hahn
Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
1 shared publication - Megan Kern
The University of North Carolina at Chapel Hill
1 shared publication
Latest Publications
- Open Access
Combined Atomic Force Microscope and Volumetric Light Sheet System for Mechanobiology
The central goals of mechanobiology are to understand how cells generate force and how they respond to environmental mechanical stimuli. A full picture of these processes requires high-resolution, volumetric imaging with time-correlated force measurements. Here we present an instrument that combines an open-top, single-objective light sheet fluorescence microscope with an atomic force microscope (AFM), providing simultaneous volumetric imaging with high spatiotemporal resolution and high dynamic range force capability (10 pN – 100 nN). With this system we have captured lysosome trafficking, vimentin nuclear caging, and actin dynamics on the order of one second per volume. To showcase the unique advantages of combining Line Bessel light sheet imaging with AFM, we measured the forces exerted by a macrophage during FcɣR-mediated phagocytosis while performing both sequential two-color, fixed plane and volumetric imaging of F-actin. This unique instrument allows for a myriad of novel studies investigating the coupling of cellular dynamics and mechanical forces.
Open AccessCombined Atomic Force Microscope and Volumetric Light Sheet System for Mechanobiology
The central goals of mechanobiology are to understand how cells generate force and how they respond to environmental mechanical stimuli. A full picture of these processes requires high-resolution, volumetric imaging with time-correlated force measurements. Here we present an instrument that combines an open-top, single-objective light sheet fluorescence microscope with an atomic force microscope (AFM), providing simultaneous volumetric imaging with high spatiotemporal resolution and high dynamic range force capability (10 pN – 100 nN). With this system we have captured lysosome trafficking, vimentin nuclear caging, and actin dynamics on the order of one second per volume. To showcase the unique advantages of combining Line Bessel light sheet imaging with AFM, we measured the forces exerted by a macrophage during FcɣR-mediated phagocytosis while performing both sequential two-color, fixed plane and volumetric imaging of F-actin. This unique instrument allows for a myriad of novel studies investigating the coupling of cellular dynamics and mechanical forces.