Nataliia Pinchuk

The object of this study is the high-power UV radiation at different pulses and its effect on Bacillus anthracis spores. The destruction of pathogenic microorganisms at environmental objects is the key to sustainable epizootological well-being. The results of the study show that the effect of powerful pulses on the destruction of pathogenic microorganisms is achieved during a short series and even single exposures. In this case, a pulsed UV sterilizer based on a magnetoplasma compressor with a pulsed ultraviolet flux power of 3.6 MW with a wavelength of 185‒320 nm of ultraviolet radiation was used. The prospects for pulsed sterilization technology have been shown. As a result of laboratory tests, it was established that the elimination degree of spores of the test microorganism Bacillus anthracis CTI, applied to wooden plates, is lg 2.5; applied to plastic plates – lg 3.2, while the degree of death of spores applied to metal plates is lg 4.0. Based on the results, wood and plastic require more attention when choosing disinfection methods due to their lower efficiency in this context. The high resistance of spore microorganisms requires the use of high dose loads to neutralize the anthrax pathogen. The proposed disinfection methodology is relevant and can be used by veterinary medicine laboratories, livestock farms of various forms of ownership, the scientific community, and industrial enterprises manufacturing technological equipment. Further research should be aimed at devising and improving methods for combating pathogenic microorganisms and ensuring sustainable epizootic well-being

Everyone knows that test cultures are used to control and quality the growth properties of nutrient media, to check the activity of antiseptics and disinfectants, as well as to assess the adequacy of the sensitivity of the tested microorganisms to new antibacterial drugs, which are currently produced by rapid temp. with the development of antibiotic resistance of microorganisms to most of the active substances used in new drugs. Moreover, it is the understanding that most antibiotics are clinically useless in treating infectious diseases because of their long-term use for chemotherapy purposes – a major problem not only in Ukraine but worldwide. In this article the results of researches sensitivity museum strains, namely: Escherichia coli ATCC 25922 (F-50), Pseudomonas aeruginosa ATCC 2853 (F), Proteus vulgaris HX 19 number 222, Staphylococcus aureus ATCC 25923, Enterobacter aerogenes 10006, Enterococcus faecalis ATCC 19433 to the antibacterial drug “Saroflox”. We find that Saroflox inhibited the growth of all test cultures at different dilution rates. Using different concentrations of the antibiotic (2.5 mg, 1.25 mg, 0.625 mg, 0.3125 mg, 0.1562/200 μl), it was found that all test cultures under study were highly sensitive to Saroflox. most from 38.0 ± 1.0 to 20.0 ± 1.0 mm. The results of studies show that the new antibacterial drug “Saroflox” has bactericidal properties to most cultures that cause bacterial diseases of various species of animals and birds, which only confirms its effectiveness against gram-negative microorganisms (Enterobacter spp. , Staphylococcus aureus E. coli and others) including beta-lactam antibiotic resistant, tetracyclines, macrolides and aminoglycosides.