Xiaohui Du

Currently, cage housing is regarded as a global mainstream production system for laying hens. However, limited living space and confinement of birds in cages cause welfare and health problems, such as feather pecking, osteoporosis, obesity, and premature aging. Many studies have been conducted to alleviate layer welfare problems by providing farm environmental enrichments such as litter, sand, alfalfa bales, chick papers, pecking stones, pecking strings, perches, slopes, elevated platforms, aviaries and outdoor access with a trend towards complex enrichments. The provision of appropriate enrichments continuously attracts layers towards pecking, foraging, dust bathing, and locomotion, thereby giving lifelong benefits to laying hens. Hence, raising chicks and pullets under such conditions may reduce feather and skin damage, as well as accumulation of abdominal fat, and improve several biological features such as health, productivity, quality products, and docility of laying hens. Therefore, providing enrichment during the first few days of the layer’s life without any interruption is crucial. In addition, due to different farm conditions, environmental enrichment should be managed by well-trained farm staff. For example, in preventing feather pecking among the birds, litter materials for foraging are superior to dust bath materials or new items. However, a limited supply of litter creates competition and challenges among birds. Therefore, providing farm environmental enrichment for layers requires proper handling, especially in commercial layer farms. Hence, improving the welfare of chicks and pullets through optimizing on-farm environmental enrichments is essential for production systems practicing cage housing.

Studies on animal behavior and welfare have reported that improving the management practices of pullets can enhance their growth, as well as their physical and mental condition, thus benefiting the productivity of laying hens. There is growing confidence in the international community to abandon the conventional practices of “cage-rearing and beak-trimming” to improve the welfare of chickens. Therefore, in this review, we summarized some of the effective poultry management practices that have provided welfare benefits for pullets. The results are as follows: 1. Maintaining similar housing conditions at different periods alleviates fear and discomfort among pullets; 2. Pullets reared under cage-free systems have better physical conditions and temperaments than those reared in cage systems, and they are more suitable to be transferred to similar housing to lay eggs; 3. Improving flock uniformity in appearance and body size has reduced the risk of pecking and injury; 4. Maintaining an appropriate population (40–500 birds) has reduced flock aggressiveness; 5. A combination of 8–10 h of darkness and 5–30 lux of light-intensity exposure via natural or warm white LED light has achieved a welfare–performance balance in pullets. (This varies by age, strain, and activities.); 6. Dark brooders (mimicking mother hens) have alleviated fear and pecking behaviors in pullets; 7. The air quality of the chicken house has been effectively improved by optimizing feed formulation and ventilation, and by reducing fecal accumulation and fermentation; 8. Complex environments (with litter, perches, straw bales, slopes, platforms, outdoor access, etc.) have stimulated the activities of chickens and have produced good welfare effects. In conclusion, the application of comprehensive management strategies has improved the physical and mental health of pullets, which has, in turn, improved the quantity and quality of poultry products.

In this study, using a laying hen model, we determined the expression of FOXL2 and RSPO1 in different central and peripheral tissue and ovarian follicles at different stages of development. At the same time, mRNA expression of both genes in granulosa and theca cells harvested from follicles at different stages of folliculogenesis was also evaluated. Finally, we assessed the effect of leptin treatment on expression of FOXL2 and RSPO1 in in vitro cultured granulosa cells harvested from 1–5 mm to F3–F1 follicles. Our RT-qPCR results revealed that a comparatively higher expression of FOXL2 and RSPO1 was observed in ovary, hypothalamus, and pituitary. Abundant mRNA expression of FOXL2 was observed in small prehierarchical follicles (1–1.9 and 2–2.9 mm follicles; p < 0.05), whereas mRNA expression of RSPO1 showed an increasing trend in large hierarchical follicles (F5–F1), and its abundant expression was observed in post-ovulatory follicles. FOXL2 mRNA expression was stable in granulosa cells harvested from 3–5 mm to F4 follicles, and exhibited a significantly higher expression in large hierarchical follicles. Conversely, relatively low mRNA expression of FOXL2 was observed in theca cells. RSPO1 mRNA expression was relatively lower in granulosa cells; however, theca cells exhibited a significantly higher mRNA expression of RSPO1 in F4 to F1 follicles. In the next experiment, we treated the in vitro cultured granulosa cells with different concentrations (1, 10, 100, and 1000 ng/mL) of exogenous leptin. Compared to the control group, a significant increase in the expression of FOXL2 was observed in groups treated with 1, 10, and 100 ng/mL leptin, whereas expression of RSPO1 was increased in all leptin-treated groups. When treated with 100 ng/mL leptin, FOXL2 and RSPO1 expression was upregulated in cultured granulosa cells harvested from both large hierarchical (F3–F1) and small prehierarchical follicles (1–5 mm). Based on these findings and evidence from mainstream literature, we envisage that FOXL2 and RSPO1 genes (in connection with hypothalamic-hypophysis axis) and leptin (via modulation of FOXL2 and RSPO1 expression) might have significant physiological roles, at least in part, in modulating the ovarian mechanisms, such as follicle development, selection, and steroidogenesis in laying hens.

We previously showed that the fatty liver formations observed in overfed geese are accompanied by the activation of the PI3K-Akt-mTOR pathway and an increase in plasma insulin concentrations. Recent studies have suggested a crucial role for the PI3K-Akt-mTOR pathway in regulating lipid metabolism; therefore, we hypothesized that insulin affects goose hepatocellular lipid metabolism through the PI3K-Akt-mTOR signaling pathway. Goose primary hepatocytes were isolated and treated with serum-free media supplemented with PI3K-Akt-mTOR pathway inhibitors (LY294002, rapamycin, and NVP-BEZ235, respectively) and 50 or 150 nmol/L insulin. Insulin induced strong effects on lipid accumulation as well as the mRNA and protein levels of genes involved in lipogenesis, fatty acid oxidation, and VLDL-TG assembly and secretion in primary goose hepatocytes. The stimulatory effect of insulin on lipogenesis was significantly decreased by treatment with PI3K-Akt-mTOR inhibitors. These inhibitors also rescued the insulin-induced down-regulation of fatty acid oxidation and VLDL-TG assembly and secretion. These findings suggest that the stimulatory effect of insulin on lipid deposition is mediated by PI3K-Akt-mTOR regulation of lipogenesis, fatty acid oxidation, and VLDL-TG assembly and secretion in goose hepatocytes.