Agata Szymańska-Pulikowska

One of the key problems associated with the functioning of landfills is the generation of leachate. In order to reduce their negative impact on the environment, various treatment technologies are applied. Among them, solutions based on the use of phytotechnology deserve special attention. The aim of this study was to evaluate the impact of landfill leachate on the content of micro- and macroelements in plant material. The research was carried out in four municipal waste landfills located in Poland. Emergent macrophytes (P. australis) and submergent macrophytes (C. demersum) were used in this research. The migration and distribution of pollutants reaching the roots and shoots of P. australis from water solutions were also studied. The concentrations of heavy metals in the studied plants were low in all analysed cases. Higher metal contents could often be observed in roots rather than in shoots, but these differences were insignificant. The chemical composition of the studied plant samples was primarily related to the source of origin of the treated leachate (landfill), as clearly demonstrated by cluster analysis. In the conducted studies, no important differences were noted in the accumulation of the studied components between submergent plants (C. demersum) and emergent macrophytes (P. australis).

The influx of toxic pollutants into wastewater treatment plants can negatively affect the quality of the activated sludge (AS). One source is landfill leachate. The identification of microorganisms present in AS is very important, e.g., while improving wastewater treatment technology. Therefore, the aim of the study was to investigate the effect of raw leachate and after purification of Phragmites australis and Ceratophyllum demersum on the composition of the AS bacterial biocenosis. In addition, AS status was assessed by LIVE/DEAD BacLight ™ fluorescent staining. The obtained results showed that the leachate did not significantly affect the cell membranes of AS bacteria, and even a slight improvement was noted. The research carried out using the next-generation sequencing method shows that the origin of the samples (active and closed storage) and the method of processing do not significantly affect the composition of the AS bacterial biocenosis at higher taxonomic levels. However, at the species level, the appearance of bacteria not previously present in AS was observed, namely: Flavobacterium luticocti, Candidimonas nitroreducens and Nitrobacter hamburgensis. The obtained results suggest that the leachate may be a source of microorganisms positively influencing the condition of AS bacteria.

The strength and resistance of plastics at the end of their service life can hinder their degradation. The solution to this problem may be materials made of biodegradable and oxo-biodegradable plastics. The aim of this research was to determine the degree and nature of changes in the composition and structure of composted biodegradable and oxo-biodegradable bags. The research involved shopping bags and waste bags available on the Polish market. The composting of the samples was conducted in an industrial composting plant. As a result of the research, only some of the composted samples decomposed. After composting, all samples were analysed using FTIR (Fourier Transformation Infrared) spectroscopy. Carbonyl index and hierarchical cluster analysis method was used to detect similarities between the spectra of the new samples. The analysis of the obtained results showed that FTIR spectroscopy is a method that can be used to confirm the degradation and detect similarities in the structure of the analysed materials. The analysis of spectra obtained with the use of FTIR spectroscopy indicated the presence of compounds that may be a potential source of compost contamination. Plastics with certificates confirming their biodegradability and compostability should be completely biodegradable, i.e., each element used in their production should be biodegradable and safe for the environment.

Most phytoremediation studies are limited to several of the most common plants and in most cases these studies are performed to a very limited extent. There are also few publications on the assessment of the effectiveness of the removal of pollutants, performed simultaneously with various plants and using physicochemical analyses as well as toxicity tests. Therefore, the conducted studies focused on assessing the removal of pollutants from leachate by physicochemical analyses and toxicity tests. The leachate was collected from four municipal waste landfills of different ages (2 non-operational facilities and 2 active). The studies were conducted for two biological systems: using emergent macrophytes (Phragmites australis) and submergent macrophytes (Ceratophyllum demersum L.). It has been observed that efficiency of biological treatment of leachates with Phragmites australis and Ceratophyllum demersum shows significant differences at lower concentrations of solutions. Even after the treatment with Phragmites australis and Ceratophyllum demersum, the analyzed leachate samples produced a toxic effect on the test organisms. A reduction or no toxicity of the leachate after the biological treatment was observed only in some cases. The observed effects of the biological leachate treatment were not conclusive. Particular attention should be paid to the persistent toxicity, which can pose a real threat to the environment when discharging leachates after treatment.