Darci J. Phillips

This Figure illustrates spatial segregation of IL4I1+ vs FOLR2+ macrophages in BC and CRC tumor metastasis to the LN, and across eight different tumor types.

This Figure shows protein marker expression across different cell types detected in CODEX experiment, macrophage distance to the closest tumor cell, macrophage distribution across different tissue niches, and tissue niche distribution across different anatomical locations.

This Figure is a visual summary of this work’s findings and shows the spatial distribution of distinct macrophage subsets in the tumor microenvironment and the clinical significance of IL4I1+ and SPP1+ macrophages in breast and colon tumors.

Thus Figure shows spatial enrichment of NLRP3+ macrophages with neutrophils in Crohn’s Disease, and immunostimulatory and immunosuppressive protein marker expression in NLRP3+ vs SPP1+ macrophages.

Thus Figure shows CD274 gene scRNAseq expression across different cell types and datasets.

This Figure shows Kaplan-Meier plots of CRC patient samples stratified by IL4I1+ macrophage counts.

Supplementary Table 1. List of IHC antibodies

Supplementary Table 3. CODEX imiging cycles record

Thus Figure illustrates how macrophage subsets detected using scRNAseq, 4-color IF, and CODEX correspond to each other and shows representative examples of protein marker expression across macrophage niches.

This Figure shows the spatial interaction of FOLR2+ macrophages with Plasma Cells in normal colon lamina propria, normal breast, normal lymph node, and breast cancer adjacent normal tissue. It also illustrates ligand-receptor interactions predicted to occur between FOLR2+ macrophages and Plasma Cells.