Wei Liu

Controlling harmful algal blooms with algicidal bacteria is widely recognized as an effective solution. Here, an integrated transcriptomic and metabolomic approach was used to elucidate the algicidal process of Enterobacter hormaechei F2, which exhibits substantial algicidal activity. RNA sequencing identified 1,104 differentially expressed genes in the algicidal process, thus, indicating that amino acids, energy metabolism, and signaling-related genes were significantly activated during the algicidal process according to the pathway enrichment analysis. By analyzing the enriched amino acid and energy metabolism pathways using metabolomics, we identified 38 upregulated and 255 downregulated significantly changed metabolites in the algicidal process and an accumulation of B vitamins, peptides, and energy substances. The integrated analysis demonstrated that energy and amino acid metabolism, co-enzymes and vitamins, and bacterial chemotaxis are the most important pathways for the algicidal process of this strain, and as thiomethyladenosine, isopentenyl diphosphate, hypoxanthine, xanthine, nicotinamide, and thiamine, all exhibited algicidal activity.

ZnO and Er‐doped ZnO with different molar ratios of Er/Zn were prepared using the homogeneous precipitation method. The photocatalysts prepared were characterized using scanning electron microscopy, transmission electron microscopy, X‐ray diffraction (XRD), UV‐vis spectroscopy, and photoluminescence spectroscopy. The results showed that the Er‐doped ZnO displayed characteristic wurtzite‐type peaks in the XRD spectra. The Er‐doped ZnO absorbed much more light than ZnO in the ultraviolet region. And the doping of Er into ZnO enhanced the intensity of the fluorescence emission. The degradation of methylene blue (MB) solution demonstrated that the photocatalytic activity of ZnO was significantly improved with Er doping.