Joanna Ziemska

Graduation towers are structures that saturate the air with a brine aerosol, mimicking a coastal microclimate. They are increasingly attracting interest for both their therapeutic potential and recreational use. However, scientific evidence supporting the effectiveness of inhalation therapy using brine graduation towers is limited, as are data on methods for determining the mineral composition of the brine aerosol. The aim of this study was to evaluate an analytical method for determining the chemical composition of aerosols generated by brine graduation towers, in which water-soluble ionic species are extracted using a gas washing bottle equipped with a sintered glass disc. Water-soluble components were collected by drawing air through the gas washing bottle filled with deionised water. The method parameters were first optimised in a purpose-built chamber generating a controlled aerosol stream. Following laboratory experiments, the procedure was validated under field conditions to determine the major ionic constituents of the air at a health resort brine graduation tower. Collected aerosol samples were analysed using high-performance ion chromatography (HPIC), flame photometry, and inductively coupled plasma optical emission spectrometry (ICP-OES). The mean concentrations were 5.2 mg/m³ for sodium, 9.4 mg/m³ for chloride, and 0.37 mg/m³ for calcium. Concentrations of potassium, magnesium, arsenic, cadmium, cobalt, chromium, copper, iron, manganese, nickel, lead, antimony, sulphate(VI), nitrate(V), and phosphate(V) were below the detection limits.

Balneotherapy, utilising natural medicinal waters, gases, and peloids, is a clinically effective method for complementing the treatment of various medical conditions. One specific type of balneotherapy is crenotherapy, which involves the oral administration of natural medicinal waters. This study aimed to assess the impact of storage time on the stability of the chemical composition and microbiological quality of bottled natural medicinal waters, as well as to assess the feasibility of using packaged natural medicinal waters at home to prolong the effect post-sanatorium treatment, as recommended by the chief physician responsible for health resorts. Fourteen bottled medicinal waters sourced from seven Polish health resorts were analysed. The study examined the following samples: one HCO3-Cl-Na-Ca, four HCO3-Cl-Na, one HCO3-Mg-Na-Ca, two HCO3-Na-Ca, two HCO3-Ca, two HCO3-Na and two Cl-Na waters. Physicochemical and microbiological parameters were measured at the following times: t0 (immediately after packaging), t1 (midway through the declared shelf life) and t2 (at the end of the shelf life). Physicochemical analysis of the medicinal waters demonstrated the stability of the chemical composition in terms of basic minerals and physicochemical parameters over the product’s shelf life, as defined by the manufacturer. Faecal indicator bacteria or Pseudomonas aeruginosa were absent in all of the samples tested. However, coliform bacteria, potentially of biofilm origin, were detected in one sample. An initially high number of microorganisms, determined at 22°C, observed in three water samples, was no longer detected at the final testing stage. The findings indicate that the medicinal waters analysed maintain stability in chemical composition, organoleptic parameters and microbiological purity throughout the manufacturer’s specified shelf life.

Cancers are the leading cause of deaths worldwide. In 2018, an estimated 18.1 million new cancer cases and 9.6 million cancer-related deaths occurred globally. Several previous studies have shown that the enzyme, leucine aminopeptidase is involved in pathological conditions such as cancer. On the basis of the knowledge that isoquinoline alkaloids have antiproliferative activity and inhibitory activity towards leucine aminopeptidase, the present study was conducted a study which involved database search, virtual screening, and design of new potential leucine aminopeptidase inhibitors with a scaffold based on 3,4-dihydroisoquinoline. These compounds were then filtered through Lipinski’s “rule of five,” and 25 081 of them were then subjected to molecular docking. Next, three-dimensional quantitative structure-activity relationship (3D-QSAR) study was performed for the selected group of compounds with the best binding score results. The developed model, calculated by leave-one-out method, showed acceptable predictive and descriptive capability as represented by standard statistical parameters r² (0.997) and q² (0.717). Further, 35 compounds were identified to have an excellent predictive reliability. Finally, nine selected compounds were evaluated for drug-likeness and different pharmacokinetics parameters such as absorption, distribution, metabolism, excretion, and toxicity. Our methodology suggested that compounds with 3,4-dihydroisoquinoline moiety were potentially active in inhibiting leucine aminopeptidase and could be used for further in-depth in vitro and in vivo studies.

Secondary metabolites obtained from Actinomycetales provide a potential source of many new antibacterial, antitumour, antifungal, antiviral, antiparasitic compounds and others. The majority of discovered substances are widely used as medicines for combating multidrug-resistant Gram-positive and Gram-negative bacterial strains, as well as in veterinary practice, agriculture and industry. Members of the Streptomyces genus are outstanding producers of already known secondary metabolites. In our study we isolated a new metabolite from Streptomyces badius fermentation broth. This compound, 2-acetamido-5-hydroxybenzoic acid (1) was already described in literature, however, not yet discovered as Streptomyces badius product. The compound was firstly isolated from fermentation broth and purified by chromatography methods (ion exchange resin DowexWX40, Solid Phase Extraction C18 Polar Plus; HPLC, dC18). Then, the chemical structure of metabolite was determined by ¹H NMR, ¹³C NMR and 2D homo- and heteronuclear (¹H-¹³C HSQC, ¹H-¹³C HMBC) NMR. The molecular formula of (1), C₉H₉NO₄ was identified by high resolution ESI-MS. The chemical structure was analyzed by the UV and IR methods, as well. Biological activities of 2-acetamido-5-hydroxybenzoic acid were evaluated. The compound shows moderate DD-peptidase 64-575 inhibitory activity as well as antioxidative properties (ABTS radical scavenging). Such chemical moiety may serve as model compound for further modern drug discovery and be a source of active substance in anti-ageing cosmetics.

The nutritional requirements and environmental conditions for a submerged culture of Streptomyces sp. 8812 were determined. Batch and fed-batch Streptomyces sp. 8812 fermentations were conducted to obtain high activity of secondary metabolites. In the study several factors were examined for their influence on the biosynthesis of the active metabolites-7-hydroxy-6-oxo-2,3,4,6-tetrahydroisoquinoline-3-carboxyl acid (C ₁₀ H ₉ NO ₄ ) and N-acetyl-3,4-dihydroxy-l-phenylalanine (C ₁₁ H ₁₃ NO ₅ ): changes in medium composition, pH of production medium, various growth phases of seed culture, amino acid supplementation and addition of anion exchange resin to the submerged culture. Biological activities of secondary metabolites were examined with the use of dd-carboxypeptidase 64–575 and horseradish peroxidase. Streptomyces sp. 8812 mycelium was evaluated under fluorescent microscopy and respiratory activity of the strain was analyzed. Moreover, the enzymatic profiles of the strain with the use of Api®ZYM test were analyzed and genetic analysis made. Phylogenetic analysis of Streptomyces sp. 8812 revealed that its closest relative is Streptomyces capoamus JCM 4734 (98%), whereas sequence analysis for 16S rRNA gene using NCBI BLAST algorithm showed 100% homology between these two strains. Biosynthetic processes, mycelium growth and enzyme inhibitory activities of these two strains were also compared.

A series of 3,4-dihydroisoquinoline-3-carboxylic acid derivatives were synthesised and tested for their free-radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH^·), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS^·+), superoxide anion radical (O₂^·−) and nitric oxide radical (^·NO) assays. We also studied D-amino acid oxidase (DAAO), acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activity. Almost each of newly synthesised compounds exhibited radical scavenging capabilities. Moreover, several compounds showed moderate inhibitory activities against DAAO, AChE and BuChE. Compounds with significant free-radical scavenging activity may be potential candidates for therapeutics used in oxidative-stress-related diseases.

Bacterial resistance to commonly used antibiotics is constantly increasing. Bacteria particularly dangerous for human life are methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus faecium and fluoroquinolone-resistant Pseudomonas aeruginosa. Hence, there is an incessant need for developing compounds with new modes of action and seeking alternate drug targets. In this review, the authors discuss the current situation of antibacterial medicines and present data on new antibiotic targets. Moreover, alternatives to antibiotics, such as bacteriophages, antimicrobial peptides and monoclonal antibodies, are presented. The authors also draw attention to the valuable features of natural sources in developing antibacterial compounds. The need to prevent and control infections as well as the reasonable use of currently available antibiotics is also emphasized.