Agata Stefanek
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Agata Stefanek has a total of 18 co-authors. Here are the top co-authors by number of shared publications:
- Agnieszka Chodara
Institute of High Pressure Physics, Polish Academy of Sciences, Sokolowska 29/37, 01-142 Warsaw, Poland
1 shared publication - Ahmed Mahmoud Aboul-Enein
Department of Biochemistry, Faculty of Agriculture, Cairo University, Giza, Egypt
1 shared publication Aleksandra Kulikowska-Darłak
Warsaw Univ Technol, Fac Chem & Proc Engn, Biomed Engn Lab, PL-00645 Warsaw, Poland
1 shared publicationAleksandra Nowak
Warsaw Univ Technol, Fac Chem & Proc Engn, Biomed Engn Lab, PL-00645 Warsaw, Poland
1 shared publication- Anna Swiderska-Sroda
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, Sokolowska 29/37, 01-142 Warsaw, Poland
1 shared publication - Faten Abou-Elella
Biochemistry Department, Faculty of Agriculture, Cairo University, P.C. 12613 Giza, Egypt
1 shared publication
Latest Publications
- 6 November 2023
- Published by Polish Academy of Sciences Chancellery in Chemical and Process Engineering: New Frontiers
- Vol. 43 (1) , 81-95
Biomimetic alginate/perfluorocarbon microcapsules – the effect on in vitro metabolic activity and long-term cell culture
- 6 November 2023
- Published by Polish Academy of Sciences Chancellery in Chemical and Process Engineering: New Frontiers
- Vol. 43 (1) , 81-95
Biomimetic alginate/perfluorocarbon microcapsules – the effect on in vitro metabolic activity and long-term cell culture
- Open Access
- 16 January 2020
- Published by MDPI AG in Pharmaceutics
- Vol. 12 (1) , 70
Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles
Targeted drug delivery offers great opportunities for treating cancer. Here, we developed a novel anticancer targeted delivery system for piperine (Pip), an alkaloid prodrug derived from black pepper that exhibits anticancer effects. The tailored delivery system comprises aggregated hydroxyapatite nanoparticles (HAPs) functionalized with phosphonate groups (HAP-Ps). Pip was loaded into HAPs and HAP-Ps at pH 7.2 and 9.3 to obtain nanoformulations. The nanoformulations were characterized using several techniques and the release kinetics and anticancer effects investigated in vitro. The Pip loading capacity was >20%. Prolonged release was observed with kinetics dependent on pH, surface modification, and coating. The nanoformulations fully inhibited monolayer HCT116 colon cancer cells compared to Caco2 colon cancer and MCF7 breast cancer cells after 72 h, whereas free Pip had a weaker effect. The nanoformulations inhibited ~60% in HCT116 spheroids compared to free Pip. The Pip-loaded nanoparticles were also coated with gum Arabic and functionalized with folic acid as a targeting ligand. These functionalized nanoformulations had the lowest cytotoxicity towards normal WI-38 fibroblast cells. These preliminary findings suggest that the targeted delivery system comprising HAP aggregates loaded with Pip, coated with gum Arabic, and functionalized with folic acid are a potentially efficient agent against colon cancer.
- 16 January 2020
- Published by MDPI AG in Pharmaceutics
- Vol. 12 (1) , 70
Open AccessEffective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles
Targeted drug delivery offers great opportunities for treating cancer. Here, we developed a novel anticancer targeted delivery system for piperine (Pip), an alkaloid prodrug derived from black pepper that exhibits anticancer effects. The tailored delivery system comprises aggregated hydroxyapatite nanoparticles (HAPs) functionalized with phosphonate groups (HAP-Ps). Pip was loaded into HAPs and HAP-Ps at pH 7.2 and 9.3 to obtain nanoformulations. The nanoformulations were characterized using several techniques and the release kinetics and anticancer effects investigated in vitro. The Pip loading capacity was >20%. Prolonged release was observed with kinetics dependent on pH, surface modification, and coating. The nanoformulations fully inhibited monolayer HCT116 colon cancer cells compared to Caco2 colon cancer and MCF7 breast cancer cells after 72 h, whereas free Pip had a weaker effect. The nanoformulations inhibited ~60% in HCT116 spheroids compared to free Pip. The Pip-loaded nanoparticles were also coated with gum Arabic and functionalized with folic acid as a targeting ligand. These functionalized nanoformulations had the lowest cytotoxicity towards normal WI-38 fibroblast cells. These preliminary findings suggest that the targeted delivery system comprising HAP aggregates loaded with Pip, coated with gum Arabic, and functionalized with folic acid are a potentially efficient agent against colon cancer.