Görkem Kismali

Natural products are widely used in the treatment of cancer due to the side effects of chemotherapeutics, and a large number of natural compounds have been reported to have anticancer activities in different types of cancer. The aim of the study was to analyze the antiproliferative and apoptotic effects of capsaicin and alpha-lipoic acid on prostate cancer cells in silico and in vitro. The effects of capsaicin and alpha-lipoic acid on the proliferation of prostate cancer cells were assessed using MTT cell viability assays. Apoptotic protein levels were measured using Western blot analysis. Ligand–protein potential interactions of alpha-lipoic acid and capsaicin with survivin and bax proteins were examined using CB-Dock2 and SwissDock software. Capsaicin and alpha-lipoic acid significantly inhibited the proliferation of both prostate cancer cell lines in a dose- and time-dependent manner. Our data revealed that various concentrations of capsaicin, alpha-lipoic acid and their combinations caused remarkable downregulation of survivin expression on prostate cancer cells. According to the Vina scores, alpha-lipoic acid and capsaicin have the potential to interact strongly with survivin and bax proteins. We suggest that both natural compounds have the potential for treating prostate cancer according to the in vitro and in silico results.

In this study, the alteration of paraoxonase, ceruloplasmin and immunoglobulin G levels in hair goats at different ages were investigated. 88 hair goats grazed in the same season in Adana (Turkey) were included in the study. Goats were divided into five groups as 0-6 months old, 7-12 months old, 1.5-2 years old, 2.5-6 years old and 7< years old. Paraoxonase activity and ceruloplasmin levels were measured by spectrophotometer and immunoglobulin G was measured with ELISA kit. Although there was no statistically significant difference between the groups in paraoxonase activity, it increased until 6 years of age, but decreased with aging. A statistically significant difference was found between the groups in ceruloplasmin levels. While it provided a decrease in values up to the age of 2 and in old age, a little increase was observed in the fourth group. A statistically significant difference was found between the groups in immunoglobulin G levels, and it was observed that immunoglobulin G levels increased from newborns to adulthood, decreased with age, but increased again with aging. As a result, aging actually means an increase in reactive oxygen species, the emergence of diseases, and the loss of function of tissues and cells. Based on this, it can be said that as you get older, the body will become open to microorganisms and the emergence of diseases will increase. These results suggest that immunoglobulin G increase with age to protect the body. Paraoxonase and ceruloplasmin levels also showed significant change with age. However, more extensive studies are needed to reveal the cause more precisely.

Infectious diseases are lately considered as one of the most important global risks which negatively impact not only the health but also the economy of countries. Globalization influences spread of infectious diseases as a result of new patterns of human travel and increased human interaction. Thus, in the new world, it is very important to prevent and diagnose the new infectious diseases by using accurate and quick diagnostic methods. Bio-detection dogs have a great potential to accurately diagnose infectious disease as they have a great ability to discriminate the volatile organic compounds (VOCs) which are specific to disease. This review discusses the potential use of bio-detection dogs in prevention and diagnosing of infectious disease. Moreover, factors affecting the scent of disease, i.e. VOCs, are tried to be highlighted. Keywords: Bio-detection dogs, diagnosis, infectious disease, volatile organic compound (VOC)

Ascorbic Acid and selenium has a potential use in the prevention of cancer. The role of antioxidant enzymes against cancer is to prevent oxidative damage. The aim of the study was to investigate the effect of Ascorbic Acid and selenium on antioxidant enzyme activities in HepG2 cell line. In this study, the effects of Org Se (234 µM), SeO2 (100 µM), Ascorbic Acid (31,3 mM) and Ascorbic Acid (0,313 mM) on antioxidant enzyme activities in HepG2 cell line were studied. GPx, CAT and SOD activities were measured spectrophotometrically. There was a significant increase in the activity of GPx in HepG2 cells treated with Org Se (234 µM), SeO2 (100 µM), Ascorbic Acid (31,3 mM) and Ascorbic Acid (0,313 mM), however the activity of CAT was found significantly decreased in Org Se (234 µM), SeO2 (100 µM), Ascorbic Acid (31,3 mM) treated HepG2 cells. The SOD levels of HepG2 cells treated with Ascorbic Acid (31,3 mM) and AA (0,313 mM) were found higher than the control cells. The results of the present study indicated that the antioxidant enzyme activities changed depending on Org Se, SeO2 application and CAT activities changed depending on Ascorbic Acid doses.

En tr The aim of the present study is to investigate the effects of three different steroid hormones; 17 β estradiol, Diethylstilbestrol and progesterone on cell viability in F98 glioblastoma cells. F98 glioblastoma cells were treated with different concentrations of Progesterone (10, 20, 50, 100 μM), DES (2.5, 5, 10, 20, 50, 100 μM) and 17β estradiol (0.01, 0.1, 1, 10 μM)) for 24, 48 and 72 hours and MTT assay was applied to determine thecell viability. Progesterone inhibits glioblastoma cell growth in a dose and time dependent manner. Antiproliferative effect of 17 β estradiol was observed at low doses. Biphasic distribution was observed in decreasing cell viability in DES applications. These results suggest that Progesterone, 17β estradiol and DES can inhibit the proliferation of glioblastoma cells. However, further study is necessary to identify the pathways involved. Bu çalışmada 17 β östradiol, Dietilsitilbesterol ve progesterone gibi steroid hormonların F98 glioblostomo hücre hatlarında hücre canlılığı üzerine etkilerinin araştırılması amaçlanmıştır. F98 glioblastoma hücrelerine farlı dozlarda Progesteron (10, 20, 50, 100 μM), DES (2.5, 5, 10, 20, 50, 100 μM) ve 17β estradiol (0.01, 0.1, 1, 10 μM) 24, 48 ve 72 saat süre ile uygulanmış ve hücre canlılığının belirlenmesi amacıyla MTT hücre canlılık testi uygulanmıştır. Progesteron glioblostoma hücrelerinin büyümesini doz ve zaman bağımlı olarak inhibe etmiştir. 17 β estradiol düşük dozlarda antiproliferatif etki göstermiştir. DES uygulamaları hücre canlılığı üzerinde iki yönlü etki göstermiştir. Elde edilen sonular Progesterone, 17β estradiol ve DES’in glioblostoma hücrelerinde hücre çoğalmasını inhibe ettiğini göstermektedir. Ancak bununla ilgili yolakların belirlenmesi için daha fazla çalışma yapılmasına ihtiyaç vardır. Bu çalışmada 17 β östradiol, Dietilsitilbesterol ve progesteron gibi steroid hormonların F98 glioblostomo hücre hatlarında hücre canlılığı üzerine etkilerinin araştırılması amaçlanmıştır. F98 glioblastoma hücrelerine farlı dozlarda Progesteron (10, 20, 50, 100 μM), DES (2.5, 5, 10, 20, 50, 100 μM) ve 17β estradiol (0.01, 0.1, 1, 10 μM) 24, 48 ve 72 saat süre ile uygulanmış ve hücre canlılığının belirlenmesi amacıyla MTT hücre canlılık testi uygulanmıştır. Progesteron glioblostoma hücrelerinin büyümesini doz ve zaman bağımlı olarak inhibe etmiştir. 17 β estradiol düşük dozlarda antiproliferatif etki göstermiştir. DES uygulamaları hücre canlılığı üzerinde iki yönlü etki göstermiştir. Elde edilen sonular Progesteron, 17β estradiol ve DES’in glioblostoma hücrelerinde hücre çoğalmasını inhibe ettiğini göstermektedir. Ancak bununla ilgili yolakların belirlenmesi için daha fazla çalışma yapılmasına ihtiyaç vardır.

Vitamin A, calcium (Ca), phosphorus (P) and magnesium (Mg) are essential components for the health and reproductive yield of dairy cows. In this study, it is aimed to profile the calcium, phosphorus and magnesium elements together with vitamin A, which are important components in cattle bred and reared in Northern Cyprus. To analyse these parameters, 260 clinically healthy animals, at least 30 from each region, were blood sampled from eight different regions (Nicosia, Gecitkale, Vadili, Famagusta, Iskele, Ziyamet, Morphou and Kyrenia) during both summer and winter seasons. Vitamin A, calcium, magnesium and phosphorus concentrations were measured from blood samples. Vitamin A levels increased significantly only in Nicosia and Ziyamet regions during the winter season, while there was no seasonal difference from the other regions. Calcium and phosphorus levels were higher in summer when compared with winter. Magnesium levels were significantly higher in winter than in summer. In the comparison between regions in summer and winter, the change in P and Mg values was significant, whereas Ca only showed inter-regional differences during winter. In conclusion, all the parameters found were within the expected ranges but affected by seasonal changes. Therefore, we think that calcium and phosphorus supplementation in winter and vitamin A and magnesium supplementation in summer will provide positive results on cattle.

Natural products and anti-inflammatory agents including cyclooxygenase-2 (COX-2) inhibitors which is a type of nonsteroidal anti-inflammatory drugs (NSAIDs) are highly considerable interest for the prevention of carcinogenesis. The objective of this study is to evaluate the oxidative status of colitis-associated cancer induced by azoxymethane (AOM)/dextran sulfate sodium (DSS), and the effects of COX-2 inhibitor in mice. Totally 40 mice were randomized and divided to four groups. All animals except control and Cox-2 inhibitor alone group received AOM/DSS to establish colitis-associated cancer model as reported elsewhere. COX-2 preferential inhibitor meloxicam was used to minimize side effects such as gastrointestinal hemorrhage. Meloxicam were used (5mg/kg, intraperitoneal) three times a week with meloxicam alone and AOM/DSS + meloxicam group. Superoxide dismutase (SOD), Glutathione peroxidase (GPx), Malondialdehyde (MDA) and Advanced Oxidation Protein Products (AOPP) which all of them are oxidative stress markers were measured by spectrophotometrically. The combination treatment of Meloxicam and AOM/DSS significantly increased (P< 0.05) SOD activities in mice. GPx activities were found significantly increased (P< 0.05) in Meloxicam and AOM/DSS combinations or alone. There were no differences between the control and treatment groups of MDA levels. AOPP levels of Meloxicam and AOM/DSS combination group were found higher than the other groups. Meloxicam and /or AOM/DSS treatment not caused lipid peroxidations, but increased the antioxidant enzymes and Advanced Oxidation Protein Products levels.