Saet-Byul Park
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Saet-Byul Park has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Hae-Yeong Kim
Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea
2 shared publications - Do-Geun Lee
Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea
1 shared publication - Jae-Hwan Kim
Pinucle, Yongin 16870, Korea
1 shared publication - Ji-Yeong Kim
Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea
1 shared publication - Mi-Ju Kim
Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin, South Korea
1 shared publication - Min-Ki Shin
Novel Food Division, National Institute of Food and Drug Safety Evaluation, Cheongju, Korea
1 shared publication
Latest Publications
- Open Access
Development of a Systematic qPCR Array for Screening GM Soybeans
A screening method using the 35S promoter and nos terminator for genetically modified organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time polymerase chain reaction (also known as quantitative polymerase chain reaction, qPCR) array targeting eight screening assays combined with a prediction system was developed for the rapid tracking of GM soybeans. Each assay’s specificity was tested and confirmed using 17 GM soybean events that have been approved in Korea. The sensitivity of each assay was determined to range from 0.01% to 0.05% using DNA mixtures with different GM ratios, and it was validated by the results of three experimenters. The applicability of this study was tested by monitoring 23 processed foods containing soybeans. It was figured out that 13 of the 23 samples included GM soybeans. The prediction system combined with screening results will be helpful to trace the absence/presence of GM soybean events. This new qPCR array and prediction system for GM soybean detection provides rapid, convenient and reliable results to users.
Open AccessDevelopment of a Systematic qPCR Array for Screening GM Soybeans
A screening method using the 35S promoter and nos terminator for genetically modified organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time polymerase chain reaction (also known as quantitative polymerase chain reaction, qPCR) array targeting eight screening assays combined with a prediction system was developed for the rapid tracking of GM soybeans. Each assay’s specificity was tested and confirmed using 17 GM soybean events that have been approved in Korea. The sensitivity of each assay was determined to range from 0.01% to 0.05% using DNA mixtures with different GM ratios, and it was validated by the results of three experimenters. The applicability of this study was tested by monitoring 23 processed foods containing soybeans. It was figured out that 13 of the 23 samples included GM soybeans. The prediction system combined with screening results will be helpful to trace the absence/presence of GM soybean events. This new qPCR array and prediction system for GM soybean detection provides rapid, convenient and reliable results to users.
- 16 March 2019
- Published by Springer Nature in Food Science and Biotechnology
- Vol. 28 (5) , 1593-1598
Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR
Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes Cyclophilin (tomato), Mdtl 1 (apple), Pru p 2.01A (peach) and Pectin methylesterase inhibitor (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.
- 16 March 2019
- Published by Springer Nature in Food Science and Biotechnology
- Vol. 28 (5) , 1593-1598
Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR
Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes Cyclophilin (tomato), Mdtl 1 (apple), Pru p 2.01A (peach) and Pectin methylesterase inhibitor (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.