Alfonso González-Gil
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Alfonso González-Gil has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Celia Garcia-Hernandez
Group UVASENS, Escuela de Ingenierías Industriales, Universidad de Valladolid, Paseo del Cauce, 59, 47011 Valladolid, Spain
1 shared publication - Coral Salvo-Comino
Group UVASENS, Escuela de Ingenierías Industriales, Universidad de Valladolid, Paseo del Cauce, 59, 47011 Valladolid, Spain
1 shared publication - Cristina Garcia-Cabezon
Dpt. Materials Science Escuela de Ingenierías Industriales, University of Valladolid, Valladolid, Spain; Group UVASENS, Dpt. Inorganic Chemistry. Escuela de Ingenierías Industriales, University of Valladolid, Valladolid, Spain; BioecoUVA Research Institute, University of Valladolid, Valladolid, Spain
1 shared publication - Fernando Martin
Materials Engineering, Escuela de Ingenierías Industriales, Universidad de Valladolid, 47011 Valladolid, Spain
1 shared publication - Javier Rodriguez-Valentin
Group UVASENS, Escuela de Ingenierías Industriales, Universidad de Valladolid, Paseo del Cauce, 59, 47011 Valladolid, Spain
1 shared publication - Maria Luz Rodriguez-Mendez
Universidad de Valladolid
1 shared publication
Latest Publications
- Open Access
Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure
Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.
Open AccessBiosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure
Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.