Denis Fernando Biffe
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The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Denis Fernando Biffe has a total of 47 co-authors. Here are the top co-authors by number of shared publications:
- Rubem Silvério de Oliveira Junior
Universidade Estadual de Maringá, Brazil
13 shared publications - Jamil Constantin
Universidade Estadual de Maringá
10 shared publications - Luiz Augusto Inojosa Ferreira
Universidade Estadual de Maringá, Brazil
5 shared publications - Daniel Nalin
Center of Nuclear Energy in Agriculture, University of São Paulo, Av. Centenário 303, Piracicaba 13400-970, SP, Brazil
4 shared publications - Guilherme Braga Pereira Braz
Universidade de Rio Verde
4 shared publications - Rafael Romero Mendes
Universidade Estadual de Maringá, Brazil
4 shared publications
Latest Publications
- Open Access
- 2 January 2026
- Published by Brazilian Journals in Caderno Pedagógico
- Vol. 23 (1) , e22965
Commelina benghalensis: Aspectos de biologia e manejo
Trapoeraba (Commelina benghalensis) é a espécie mais relevante do gênero Commelina em áreas agrícolas brasileiras, destacando-se pela ampla distribuição, elevada capacidade reprodutiva e dificuldade de controle. Pertencente à família Commelinaceae, apresenta metabolismo C3, reprodução sexuada e assexuada e características morfológicas únicas, como flores aéreas e subterrâneas cleistogâmicas, que contribuem para sua persistência no solo. No Brasil, ocorre em praticamente todo o território, sendo problemática especialmente nas regiões Sudeste, Sul e Centro-Oeste. A diferenciação entre C. benghalensis e espécies afins (C. diffusa, C. erecta e C. villosa) é essencial para o manejo, sendo possível por meio de características como hábito de crescimento, pilosidade, morfologia das flores e espatas, sistema radicular e presença exclusiva de flores subterrâneas em C. benghalensis. A espécie apresenta tolerância ao herbicida glyphosate que aumenta conforme a planta desenvolve e ainda, apresenta estruturas em sua folha que afetam a absorção do herbicida. Essas particularidades tornam a espécie altamente competitiva e desafiadora às práticas convencionais de manejo. Esta revisão tem como objetivo reunir aspectos biológicos de C. benghalensis, destacando características que influenciam nas estratégias de controle.
- 2 January 2026
- Published by Brazilian Journals in Caderno Pedagógico
- Vol. 23 (1) , e22965
Open AccessCommelina benghalensis: Aspectos de biologia e manejo
Trapoeraba (Commelina benghalensis) é a espécie mais relevante do gênero Commelina em áreas agrícolas brasileiras, destacando-se pela ampla distribuição, elevada capacidade reprodutiva e dificuldade de controle. Pertencente à família Commelinaceae, apresenta metabolismo C3, reprodução sexuada e assexuada e características morfológicas únicas, como flores aéreas e subterrâneas cleistogâmicas, que contribuem para sua persistência no solo. No Brasil, ocorre em praticamente todo o território, sendo problemática especialmente nas regiões Sudeste, Sul e Centro-Oeste. A diferenciação entre C. benghalensis e espécies afins (C. diffusa, C. erecta e C. villosa) é essencial para o manejo, sendo possível por meio de características como hábito de crescimento, pilosidade, morfologia das flores e espatas, sistema radicular e presença exclusiva de flores subterrâneas em C. benghalensis. A espécie apresenta tolerância ao herbicida glyphosate que aumenta conforme a planta desenvolve e ainda, apresenta estruturas em sua folha que afetam a absorção do herbicida. Essas particularidades tornam a espécie altamente competitiva e desafiadora às práticas convencionais de manejo. Esta revisão tem como objetivo reunir aspectos biológicos de C. benghalensis, destacando características que influenciam nas estratégias de controle.
- Open Access
- 2 January 2026
- Published by Brazilian Journals in Caderno Pedagógico
- Vol. 23 (1) , e22798
Selection of herbicide for pre-emergence control of volunteer soybean (Glycine max) in maize (Zea mays)
Volunteer soybean is the main weed competing with second-crop maize in Brazil, and options for early control are still limited. Two field trials were carried out in Mandaguaçu (PR) in 2023: one evaluating maize selectivity and another assessing volunteer soybean control. In the selectivity trial, only ametryn and amicarbazone were not selective, reducing maize yield. In the control trial, no treatment achieved 100% control, but amicarbazone (140 and 280 g a.i. ha⁻¹), tembotrione (201.6), terbuthylazine (2800), tembotrione + atrazine (100.8 + 1500) and mesotrione + terbuthylazine (192 + 900) provided efficient control (≥80%) up to 28 DAA. Treatments offering at least 55% early control also resulted in yields similar to the weeded control. Integrating both trials, the best overall results were obtained with atrazine (2500, 5000), mesotrione (192), mesotrione + atrazine (100 + 1000), mesotrione + terbuthylazine (192 + 900), tembotrione, tembotrione + atrazine, terbuthylazine and terbuthylazine + s-metolachlor.
- 2 January 2026
- Published by Brazilian Journals in Caderno Pedagógico
- Vol. 23 (1) , e22798
Open AccessSelection of herbicide for pre-emergence control of volunteer soybean (Glycine max) in maize (Zea mays)
Volunteer soybean is the main weed competing with second-crop maize in Brazil, and options for early control are still limited. Two field trials were carried out in Mandaguaçu (PR) in 2023: one evaluating maize selectivity and another assessing volunteer soybean control. In the selectivity trial, only ametryn and amicarbazone were not selective, reducing maize yield. In the control trial, no treatment achieved 100% control, but amicarbazone (140 and 280 g a.i. ha⁻¹), tembotrione (201.6), terbuthylazine (2800), tembotrione + atrazine (100.8 + 1500) and mesotrione + terbuthylazine (192 + 900) provided efficient control (≥80%) up to 28 DAA. Treatments offering at least 55% early control also resulted in yields similar to the weeded control. Integrating both trials, the best overall results were obtained with atrazine (2500, 5000), mesotrione (192), mesotrione + atrazine (100 + 1000), mesotrione + terbuthylazine (192 + 900), tembotrione, tembotrione + atrazine, terbuthylazine and terbuthylazine + s-metolachlor.
- Open Access
- 1 December 2025
- Published by Brazilian Journals in Contribuciones A Las Ciencias Sociales
- Vol. 18 (12) , e22810
Capim-massambará – ameaça para a agricultura brasileira
Sorghum halepense (capim-massambará) é considerada uma das plantas daninhas mais disseminadas e problemáticas nos ambientes agrícolas mundiais, impactando negativamente a produtividade e sustentabilidade dos sistemas de produção. De origem asiática, essa gramínea perene apresenta notável plasticidade ecológica, sendo capaz de colonizar diferentes condições de solo e clima devido à sua propagação tanto por sementes quanto pelo vigoroso sistema rizomático subterrâneos. Do ponto de vista morfológico, destaca-se por rizomas profundos e resistentes, colmos eretos e panículas abertas, favorecendo o estabelecimento rápido e a formação de densos povoamentos competitivos com culturas comerciais. A resistência de capim-massambará ao controle químico, em especial aos herbicidas das classes ACCase e ALS, tem sido objeto de diversos estudos recentes, indicando evolução adaptativa que compromete a efetividade desses produtos em áreas de infestações recorrentes. Tal contexto demanda abordagens integradas de manejo, que incluem estratégias químicas, mecânicas e biológicas, bem como rotação e diversificação de culturas, para o controle eficiente e sustentável da espécie. Além disso, a ingestão de Sorghum halepense por ruminantes, especialmente durante a rebrota, pode ocasionar intoxicações agudas devido ao alto teor de glicosídeos cianogênicos presentes na planta, como reportado em diferentes regiões do Brasil. Tal aspecto reforça a necessidade de manejo criterioso, evitando o uso da planta como forrageira em períodos críticos, conforme orientação de estudos bromatológicos e toxicológicos. Uma compreensão aprofundada das características biológicas, genéticas e dos mecanismos de resistência de capim-massambará é fundamental para o desenvolvimento de estratégias de manejo mais avançadas e sustentáveis, mitigando os impactos agronômicos e ambientais da espécie.
- 1 December 2025
- Published by Brazilian Journals in Contribuciones A Las Ciencias Sociales
- Vol. 18 (12) , e22810
Open AccessCapim-massambará – ameaça para a agricultura brasileira
Sorghum halepense (capim-massambará) é considerada uma das plantas daninhas mais disseminadas e problemáticas nos ambientes agrícolas mundiais, impactando negativamente a produtividade e sustentabilidade dos sistemas de produção. De origem asiática, essa gramínea perene apresenta notável plasticidade ecológica, sendo capaz de colonizar diferentes condições de solo e clima devido à sua propagação tanto por sementes quanto pelo vigoroso sistema rizomático subterrâneos. Do ponto de vista morfológico, destaca-se por rizomas profundos e resistentes, colmos eretos e panículas abertas, favorecendo o estabelecimento rápido e a formação de densos povoamentos competitivos com culturas comerciais. A resistência de capim-massambará ao controle químico, em especial aos herbicidas das classes ACCase e ALS, tem sido objeto de diversos estudos recentes, indicando evolução adaptativa que compromete a efetividade desses produtos em áreas de infestações recorrentes. Tal contexto demanda abordagens integradas de manejo, que incluem estratégias químicas, mecânicas e biológicas, bem como rotação e diversificação de culturas, para o controle eficiente e sustentável da espécie. Além disso, a ingestão de Sorghum halepense por ruminantes, especialmente durante a rebrota, pode ocasionar intoxicações agudas devido ao alto teor de glicosídeos cianogênicos presentes na planta, como reportado em diferentes regiões do Brasil. Tal aspecto reforça a necessidade de manejo criterioso, evitando o uso da planta como forrageira em períodos críticos, conforme orientação de estudos bromatológicos e toxicológicos. Uma compreensão aprofundada das características biológicas, genéticas e dos mecanismos de resistência de capim-massambará é fundamental para o desenvolvimento de estratégias de manejo mais avançadas e sustentáveis, mitigando os impactos agronômicos e ambientais da espécie.
- Open Access
- 1 January 2025
- Published by FapUNIFESP (SciELO) in Revista Caatinga
- Vol. 38, e11681
Pre-emergent herbicide screening for wheat
Weed control is a challenge in crop management due to the limited number of registered herbicides, especially for preemergent application. This study aimed to investigate the selectivity of pre-emergent herbicides with different mechanisms of action for wheat. The experiment consisted of two stages: the first involved screening under greenhouse conditions, and the second assessing the selected treatments under field conditions, with a focus on crop yield. Plant phytointoxication, crop stand, and shoot dry weight were assessed in the greenhouse experiment, and tillering, crop stand, plant height, canopy closure, yield, 1000-grain weight, and hectoliter weight in the field. Under greenhouse conditions, [imazapic + imazapyr], pendimethalin, isoxaflutole, florpyrauxifen, and halosulfuron-methyl produced the best results and were selected for the field experiment. Florpyrauxifen was the only herbicide that was selective both in greenhouse and field experiments. Isoxaflutole and trifluralin did not damage wheat in any of the field evaluations. Despite reducing crop performance in some assessments, pendimethalin and flumioxazin provided yield, hectoliter weight, and 1000-grain weight results equivalent to the herbicide-free control. Florpyrauxifen (1.08 g ha-1) was the most promising herbicide. Trifluralin (900 g ha-1), pendimethalin (1750 g ha-1), isoxaflutole (60 g ha-1), and flumioxazin (40 and 60 g ha-1) also produced grain yields equivalent to the control without herbicide.
- 1 January 2025
- Published by FapUNIFESP (SciELO) in Revista Caatinga
- Vol. 38, e11681
Open AccessPre-emergent herbicide screening for wheat
Weed control is a challenge in crop management due to the limited number of registered herbicides, especially for preemergent application. This study aimed to investigate the selectivity of pre-emergent herbicides with different mechanisms of action for wheat. The experiment consisted of two stages: the first involved screening under greenhouse conditions, and the second assessing the selected treatments under field conditions, with a focus on crop yield. Plant phytointoxication, crop stand, and shoot dry weight were assessed in the greenhouse experiment, and tillering, crop stand, plant height, canopy closure, yield, 1000-grain weight, and hectoliter weight in the field. Under greenhouse conditions, [imazapic + imazapyr], pendimethalin, isoxaflutole, florpyrauxifen, and halosulfuron-methyl produced the best results and were selected for the field experiment. Florpyrauxifen was the only herbicide that was selective both in greenhouse and field experiments. Isoxaflutole and trifluralin did not damage wheat in any of the field evaluations. Despite reducing crop performance in some assessments, pendimethalin and flumioxazin provided yield, hectoliter weight, and 1000-grain weight results equivalent to the herbicide-free control. Florpyrauxifen (1.08 g ha-1) was the most promising herbicide. Trifluralin (900 g ha-1), pendimethalin (1750 g ha-1), isoxaflutole (60 g ha-1), and flumioxazin (40 and 60 g ha-1) also produced grain yields equivalent to the control without herbicide.
- Open Access
- 1 April 2024
- Published by Universidade do Estado de Santa Catarina in Revista de Ciências Agroveterinárias
- Vol. 23 (1) , 23-32
Primeiro caso de resistência cruzada de capim-pé-de-galinha (Eleusine indica) a inibidores da ACCase no Estado do Paraná
O capim-pé-de-galinha (Eleusine indica) é uma planta que vem causando muitos problemas no Brasil, principalmente pela dificuldade de controle químico nas áreas de produção de grãos. O objetivo deste trabalho foi investigar a possível ocorrência de resistência cruzada a herbicidas inibidores da ACCase (enzima acetil-CoA carboxilase) numa população proveniente de Paranacity (PR). Os herbicidas avaliados foram fluazifop-p-butyl; quizalofop-p-ethyl, haloxyfop-p-methyl, sethoxydim e clethodim. Foram construídas curvas de dose-resposta em casa de vegetação em experimentos com delineamentos inteiramente casualizados em esquema fatorial 9x2 com quatro repetições. Os níveis do primeiro fator correspondem às doses de cada herbicida e os níveis do segundo fator referem-se aos biótipos susceptível e suspeito de resistência. As doses selecionadas corresponderam a: 0; 1/16; 1/8; 1/4; 1/2; 1; 2; 4; e 8 vezes a dose de bula de cada herbicida e todos os herbicidas foram aplicados em plantas no estádio de um perfilho. Os resultados mostraram que o biótipo proveniente de Paranacity foi suscetível ao clethodim e ao quizalofop-p-ethyl, mas apresentou dificuldade de controle com a utilização de fluazifop-p-butyl, haloxyfop-p-methyl e sethoxydim. O fator de resistência para estes herbicidas foi determinado em 10,66; 5,64; e 9,80 respectivamente. O biótipo de Eleusine indica proveniente de Paranacity foi caracterizado como resistente a fluazifop-p-butyl, haloxyfop-p-methyl e sethoxydim, constituindo o primeiro caso de resistência cruzada aos inibidores da ACCase no estado do Paraná.
- 1 April 2024
- Published by Universidade do Estado de Santa Catarina in Revista de Ciências Agroveterinárias
- Vol. 23 (1) , 23-32
Open AccessPrimeiro caso de resistência cruzada de capim-pé-de-galinha (Eleusine indica) a inibidores da ACCase no Estado do Paraná
O capim-pé-de-galinha (Eleusine indica) é uma planta que vem causando muitos problemas no Brasil, principalmente pela dificuldade de controle químico nas áreas de produção de grãos. O objetivo deste trabalho foi investigar a possível ocorrência de resistência cruzada a herbicidas inibidores da ACCase (enzima acetil-CoA carboxilase) numa população proveniente de Paranacity (PR). Os herbicidas avaliados foram fluazifop-p-butyl; quizalofop-p-ethyl, haloxyfop-p-methyl, sethoxydim e clethodim. Foram construídas curvas de dose-resposta em casa de vegetação em experimentos com delineamentos inteiramente casualizados em esquema fatorial 9x2 com quatro repetições. Os níveis do primeiro fator correspondem às doses de cada herbicida e os níveis do segundo fator referem-se aos biótipos susceptível e suspeito de resistência. As doses selecionadas corresponderam a: 0; 1/16; 1/8; 1/4; 1/2; 1; 2; 4; e 8 vezes a dose de bula de cada herbicida e todos os herbicidas foram aplicados em plantas no estádio de um perfilho. Os resultados mostraram que o biótipo proveniente de Paranacity foi suscetível ao clethodim e ao quizalofop-p-ethyl, mas apresentou dificuldade de controle com a utilização de fluazifop-p-butyl, haloxyfop-p-methyl e sethoxydim. O fator de resistência para estes herbicidas foi determinado em 10,66; 5,64; e 9,80 respectivamente. O biótipo de Eleusine indica proveniente de Paranacity foi caracterizado como resistente a fluazifop-p-butyl, haloxyfop-p-methyl e sethoxydim, constituindo o primeiro caso de resistência cruzada aos inibidores da ACCase no estado do Paraná.
- Open Access
- 1 January 2023
- Published by FapUNIFESP (SciELO) in Revista Ceres
- Vol. 70 (5) , e70519
Carryover of ACCase inhibitors for corn
Sequential crops may be affected by herbicide residuals in the soil. In corn crop there are reports of problems when the crop is sown soon after application of ACCase-inhibiting herbicides. The aim of this study was to evaluate safety intervals for the application of ACCase-inhibiting herbicides before sowing corn in different soils. Two experiments were conducted in a greenhouse, and in the field conditions. In both, the experimental design were completely randomized in factorial scheme 6x4+1 (6 periods of application x 4 herbicides + 1 control) with five replications. The treatments were: haloxyfop-P-methyl (124.7 g ha-1), clethodim – formulation 1 (192 g ha-1), quizalofop-P-ethyl (100 g ha-1) and clethodim formulation 2 (192 g ha-1) in applications at 15, 10, 5, 3, 1 and 0 days before sowing in sandy and clayey soil. In the greenhouse, formulations based on clethodim were more safety in both types of soils, and safety intervals for clethodim-based herbicides were 5 days and greater than 15 days for quizalofop-P-ethyl and haloxyfop. Haloxyfop should not be applied on day of sowing in clay soil. In the field none of the treatments compromised the development and yield of the crop, even when applied on the day of sowing.
- 1 January 2023
- Published by FapUNIFESP (SciELO) in Revista Ceres
- Vol. 70 (5) , e70519
Open AccessCarryover of ACCase inhibitors for corn
Sequential crops may be affected by herbicide residuals in the soil. In corn crop there are reports of problems when the crop is sown soon after application of ACCase-inhibiting herbicides. The aim of this study was to evaluate safety intervals for the application of ACCase-inhibiting herbicides before sowing corn in different soils. Two experiments were conducted in a greenhouse, and in the field conditions. In both, the experimental design were completely randomized in factorial scheme 6x4+1 (6 periods of application x 4 herbicides + 1 control) with five replications. The treatments were: haloxyfop-P-methyl (124.7 g ha-1), clethodim – formulation 1 (192 g ha-1), quizalofop-P-ethyl (100 g ha-1) and clethodim formulation 2 (192 g ha-1) in applications at 15, 10, 5, 3, 1 and 0 days before sowing in sandy and clayey soil. In the greenhouse, formulations based on clethodim were more safety in both types of soils, and safety intervals for clethodim-based herbicides were 5 days and greater than 15 days for quizalofop-P-ethyl and haloxyfop. Haloxyfop should not be applied on day of sowing in clay soil. In the field none of the treatments compromised the development and yield of the crop, even when applied on the day of sowing.
- Open Access
- 1 January 2023
- Published by FapUNIFESP (SciELO) in Horticultura Brasileira
- Vol. 41, e2566
Weed interference periods in lettuce crop
The knowledge of the adequate period for the beginning of weed management in lettuce crop can increase the efficiency of production, reducing the costs with management. The aim of this study was to determine the pre-interference period (PIP), the critical time for weed removal (CTWR) and the critical period of weed control (CPWC) in three lettuce cultivars, Elisa, Lucy Brown and Solaris. Coexistence and the absence of weeds were evaluated for each cultivar. The design was in randomized blocks with seven treatments (0, 7, 14, 21, 28, 35 and 42 days after transplanting - DAT) of coexistence and the same seven periods of weed absence with four replicates in each experiment. The highest infestation densities were with the species Oxalis latifolia, Coronopus didymus and Amaranthus hybridus. The leaf area and number of leaves per plant were reduced by up to 50% and 66%, respectively, in relation to the longest period of coexistence with weeds and yield reduced by 48% (Elisa), 40% (Lucy Brown) and 33% (Solaris). The cultivar Solaris showed greater tolerance to weed interference. Based on yield, PIP was 1, 3 and 3 DAT and CTWR was 37, 37 and 35 DAT, and CPWC was 2 to 37, 3 to 37 and 3 to 35 for the cultivars Elisa, Lucy Brown and Solaris, respectively.
- 1 January 2023
- Published by FapUNIFESP (SciELO) in Horticultura Brasileira
- Vol. 41, e2566
Open AccessWeed interference periods in lettuce crop
The knowledge of the adequate period for the beginning of weed management in lettuce crop can increase the efficiency of production, reducing the costs with management. The aim of this study was to determine the pre-interference period (PIP), the critical time for weed removal (CTWR) and the critical period of weed control (CPWC) in three lettuce cultivars, Elisa, Lucy Brown and Solaris. Coexistence and the absence of weeds were evaluated for each cultivar. The design was in randomized blocks with seven treatments (0, 7, 14, 21, 28, 35 and 42 days after transplanting - DAT) of coexistence and the same seven periods of weed absence with four replicates in each experiment. The highest infestation densities were with the species Oxalis latifolia, Coronopus didymus and Amaranthus hybridus. The leaf area and number of leaves per plant were reduced by up to 50% and 66%, respectively, in relation to the longest period of coexistence with weeds and yield reduced by 48% (Elisa), 40% (Lucy Brown) and 33% (Solaris). The cultivar Solaris showed greater tolerance to weed interference. Based on yield, PIP was 1, 3 and 3 DAT and CTWR was 37, 37 and 35 DAT, and CPWC was 2 to 37, 3 to 37 and 3 to 35 for the cultivars Elisa, Lucy Brown and Solaris, respectively.
- 19 October 2021
- Published by Associação Brasileira de Milho e Sorgo in Revista Brasileira de Milho e Sorgo
- Vol. 20
SELECTIVITY OF HERBICIDES APPLIED ALONE AND IN TANK MIXTURES TO SWEET SORGHUM IN POST-EMERGENCE
SELECTIVITY OF HERBICIDES APPLIED ALONE AND IN TANK MIXTURES TO SWEET SORGHUM IN POST-EMERGENCE
- 19 October 2021
- Published by Associação Brasileira de Milho e Sorgo in Revista Brasileira de Milho e Sorgo
- Vol. 20
SELECTIVITY OF HERBICIDES APPLIED ALONE AND IN TANK MIXTURES TO SWEET SORGHUM IN POST-EMERGENCE
SELECTIVITY OF HERBICIDES APPLIED ALONE AND IN TANK MIXTURES TO SWEET SORGHUM IN POST-EMERGENCE
- Open Access
- 21 September 2021
- Published by FapUNIFESP (SciELO) in Horticultura Brasileira
- Vol. 39 (3) , 305-311
Sensitivity of lettuce cultivar Lucy Brown to pre-planting herbicide application
Due to the low availability of herbicide active ingredients, the chemical weed management in lettuce crop is a challenge. The aim of this study was to evaluate the sensitivity of lettuce to the pre-planting application of the herbicides flumioxazin and trifluralin alone or in tank-mixture in lettuce, Lucy Brown cultivar. Two experiments were conducted. In experiment A, the soil was tilled before the herbicide application and it was tilled again before seedling transplanting. In experiment B, the soil was tilled just once before the herbicide application. The experimental design was in randomized blocks in a factorial scheme (3x3) +1 (2 factors with 3 levels and an additional control) with 4 replicates. We applied flumioxazin (120 g ha-1), trifluralin (450 g ha-1) and flumioxazin + trifluralin (120 + 450 g ha-1) in three different seasons before transplanting (0, 7 and 14 days). Lettuce productivity after trifluralin application (48.4 t ha-1) was higher than after flumioxazin application (16.8 t ha-1) in experiment A. In experiment B, flumioxazin decreased the number of lettuce leaves (25.6) at 14 DAT; no differences in lettuce productivity were verified, though. The herbicide trifluralin caused lower levels of phytotoxicity and was the most selective between the treatments when applied in the three seasons in both experiments.
- 21 September 2021
- Published by FapUNIFESP (SciELO) in Horticultura Brasileira
- Vol. 39 (3) , 305-311
Open AccessSensitivity of lettuce cultivar Lucy Brown to pre-planting herbicide application
Due to the low availability of herbicide active ingredients, the chemical weed management in lettuce crop is a challenge. The aim of this study was to evaluate the sensitivity of lettuce to the pre-planting application of the herbicides flumioxazin and trifluralin alone or in tank-mixture in lettuce, Lucy Brown cultivar. Two experiments were conducted. In experiment A, the soil was tilled before the herbicide application and it was tilled again before seedling transplanting. In experiment B, the soil was tilled just once before the herbicide application. The experimental design was in randomized blocks in a factorial scheme (3x3) +1 (2 factors with 3 levels and an additional control) with 4 replicates. We applied flumioxazin (120 g ha-1), trifluralin (450 g ha-1) and flumioxazin + trifluralin (120 + 450 g ha-1) in three different seasons before transplanting (0, 7 and 14 days). Lettuce productivity after trifluralin application (48.4 t ha-1) was higher than after flumioxazin application (16.8 t ha-1) in experiment A. In experiment B, flumioxazin decreased the number of lettuce leaves (25.6) at 14 DAT; no differences in lettuce productivity were verified, though. The herbicide trifluralin caused lower levels of phytotoxicity and was the most selective between the treatments when applied in the three seasons in both experiments.