Hiroshi Ando
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Hiroshi Ando has a total of 9 co-authors. Here are the top co-authors by number of shared publications:
- Hitomi Ohara
Department of Biobased Materials Science, Kyoto Institute of Technology, Kyoto, Japan
2 shared publications - Kazuhito Wada
Corporate Research & Business Division, Kaneka Corporation, Osaka 530-8288, Japan
2 shared publications - Keiji Matsumoto
Corporate Research & Business Division, Kaneka Corporation, Osaka 530-8288, Japan
2 shared publications - Mei Sano
Department of Biobased Materials Science, Kyoto Institute of Technology, Kyoto 606-8585, Japan
2 shared publications - Ryoki Yada
Department of Biobased Materials Science, Kyoto Institute of Technology, Kyoto 606-8585, Japan
2 shared publications - Takahiro Kusukawa
Faculty of Molecular Chemistry and Engineering Graduate School of Science and Technology Kyoto Institute of Technology, Matsugasaki Sakyo-ku Kyoto 606-8585 Japan
2 shared publications
Latest Publications
- Open Access
- 16 June 2020
- Published by MDPI AG in Microorganisms
- Vol. 8 (6) , 913
Correction: Sano, M., et al. Microbial Screening Based on the Mizoroki–Heck Reaction Permits Exploration of Hydroxyhexylitaconic-Acid-Producing Fungi in Soils. Microorganisms 2020, 8, 648
The authors wish to make the following correction to this paper
- 16 June 2020
- Published by MDPI AG in Microorganisms
- Vol. 8 (6) , 913
Open AccessCorrection: Sano, M., et al. Microbial Screening Based on the Mizoroki–Heck Reaction Permits Exploration of Hydroxyhexylitaconic-Acid-Producing Fungi in Soils. Microorganisms 2020, 8, 648
The authors wish to make the following correction to this paper
- Open Access
- 29 April 2020
- Published by MDPI AG in Microorganisms
- Vol. 8 (5) , 648
Microbial Screening Based on the Mizoroki–Heck Reaction Permits Exploration of Hydroxyhexylitaconic-Acid-Producing Fungi in Soils
Recently, we developed a unique microbial screening method based on the Mizoroki–Heck reaction for itaconic acid (IA)-producing fungi. This method revealed that 37 out of 240 fungal strains isolated from soils produce vinyl compounds, including IA. In this study, we further characterized these compounds in order to verify that the screening method permits the isolation of fungi that produce other vinyl compounds, excluding IA. HPLC analysis showed that 11 out of 37 isolated strains produced IA, similar to Aspergillus terreus S12-1. Surprisingly, the other 8 isolated strains produced two vinyl compounds with HPLC retention times different from that of IA. From these strains, the vinyl compounds of Aspergillus niger S17-5 were characterized. Mass spectrometric and NMR analyses showed that they were identical to 8-hydroxyhexylitaconic acid (8-HHIA) and 9-HHIA. This finding showed that 8-HHIA- and 9-HHIA-producing fungi, as well as IA-producing fungi, are ubiquitously found in soils. Neither 8-HHIA nor 9-HHIA showed antibacterial or anti-inflammatory activities. Interestingly, 8-HHIA and 9-HHIA showed cytotoxicity against the human cervical cancer cell line (HeLa) and human diploid cell line (MRC-5), and MRC-5 only, respectively, compared to IA at the same concentration. This study indicates that the screening method could easily discover fungi producing 8-HHIA and 9-HHIA in soils.
- 29 April 2020
- Published by MDPI AG in Microorganisms
- Vol. 8 (5) , 648
Open AccessMicrobial Screening Based on the Mizoroki–Heck Reaction Permits Exploration of Hydroxyhexylitaconic-Acid-Producing Fungi in Soils
Recently, we developed a unique microbial screening method based on the Mizoroki–Heck reaction for itaconic acid (IA)-producing fungi. This method revealed that 37 out of 240 fungal strains isolated from soils produce vinyl compounds, including IA. In this study, we further characterized these compounds in order to verify that the screening method permits the isolation of fungi that produce other vinyl compounds, excluding IA. HPLC analysis showed that 11 out of 37 isolated strains produced IA, similar to Aspergillus terreus S12-1. Surprisingly, the other 8 isolated strains produced two vinyl compounds with HPLC retention times different from that of IA. From these strains, the vinyl compounds of Aspergillus niger S17-5 were characterized. Mass spectrometric and NMR analyses showed that they were identical to 8-hydroxyhexylitaconic acid (8-HHIA) and 9-HHIA. This finding showed that 8-HHIA- and 9-HHIA-producing fungi, as well as IA-producing fungi, are ubiquitously found in soils. Neither 8-HHIA nor 9-HHIA showed antibacterial or anti-inflammatory activities. Interestingly, 8-HHIA and 9-HHIA showed cytotoxicity against the human cervical cancer cell line (HeLa) and human diploid cell line (MRC-5), and MRC-5 only, respectively, compared to IA at the same concentration. This study indicates that the screening method could easily discover fungi producing 8-HHIA and 9-HHIA in soils.