Ferenc Darvas
Research Subjects
Research Interests
Ferenc Darvas has a total of 9 co-authors. Here are the top co-authors by number of shared publications:
- Eugenie Nepovimova
Department of Chemistry, Faculty of Science, University of Hradec Kralove
1 shared publication - Huba Kalász
Department of Pharmacology and Pharmacotherapy, Semmelweis University, 1089 Budapest, Hungary
1 shared publication - Kamil Kuca
Department of Chemistry, University of Hradec Kralove, Hradec Kralove, Czech Republic
1 shared publication - Mamoona Shah
Department of Biosciences, COMSATS University Islamabad, Islamabad 45550, Pakistan
1 shared publication - Rabia Habib
COMSATS University Islamabad
1 shared publication - Sajida Batool
Department of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan
1 shared publication
Latest Publications
- Open Access
- 13 May 2020
- Published by MDPI AG in Biomolecules
- Vol. 10 (5) , 758
Cannabis Constituents and Acetylcholinesterase Interaction: Molecular Docking, In Vitro Studies and Association with CNR1 rs806368 and ACHE rs17228602
The study documented here was aimed to find the molecular interactions of some of the cannabinoid constituents of cannabis with acetylcholinesterase (AChE). Molecular docking and LogP determination were performed to predict the AChE inhibitory effect and lipophilicity. AChE enzyme activity was measured in the blood of cannabis addicted human subjects. Further, genetic predisposition to cannabis addiction was investigated by association analysis of cannabinoid receptor 1 (CNR1) single nucleotide polymorphism (SNP) rs806368 and ACHE rs17228602 using restriction fragment length polymorphism (RFLP) method. All the understudied cannabis constituents showed promising binding affinities with AChE and are lipophilic in nature. The AChE activity was observed to be indifferent in cannabis addicted and non-addicted healthy controls. There was no significant association with CNR1 SNP rs806368 and ACHE rs17228602. The study concludes that in silico prediction for individual biomolecules of cannabis is different from in vivo physiological action in human subjects when all are present together. However, for a deeper mechanistic insight into these interactions and association, multi-population studies are suggested. Further studies to explore the inhibitory potential of different cannabis constituents for intended AChE inhibitor-based drug are warranted.
- 13 May 2020
- Published by MDPI AG in Biomolecules
- Vol. 10 (5) , 758
Open AccessCannabis Constituents and Acetylcholinesterase Interaction: Molecular Docking, In Vitro Studies and Association with CNR1 rs806368 and ACHE rs17228602
The study documented here was aimed to find the molecular interactions of some of the cannabinoid constituents of cannabis with acetylcholinesterase (AChE). Molecular docking and LogP determination were performed to predict the AChE inhibitory effect and lipophilicity. AChE enzyme activity was measured in the blood of cannabis addicted human subjects. Further, genetic predisposition to cannabis addiction was investigated by association analysis of cannabinoid receptor 1 (CNR1) single nucleotide polymorphism (SNP) rs806368 and ACHE rs17228602 using restriction fragment length polymorphism (RFLP) method. All the understudied cannabis constituents showed promising binding affinities with AChE and are lipophilic in nature. The AChE activity was observed to be indifferent in cannabis addicted and non-addicted healthy controls. There was no significant association with CNR1 SNP rs806368 and ACHE rs17228602. The study concludes that in silico prediction for individual biomolecules of cannabis is different from in vivo physiological action in human subjects when all are present together. However, for a deeper mechanistic insight into these interactions and association, multi-population studies are suggested. Further studies to explore the inhibitory potential of different cannabis constituents for intended AChE inhibitor-based drug are warranted.