Jiye Zhang

Introduction: Phage-derived depolymerases, primarily located in phage tail fiber and tail spike proteins, have emerged as promising antibacterial and antivirulence agents capable of degrading bacterial surface polysaccharides and thereby combating multidrug-resistant bacterial infections. Despite the growing volume of publications in this interdisciplinary domain, a systematic bibliometric synthesis of the global research landscape remains absent. Methods: Publications indexed from 2010 to 2025 were retrieved from the Web of Science Core Collection (WOSCC) and Scopus on April 26, 2026. After deduplication and relevance screening, 326 records were retained. Bibliometric analyses and visualizations were performed using R with the bibliometrix package, VOSviewer and CiteSpace. Results: Annual publication output increased from 3 records in 2010 to 51 in 2025 and fitted an exponential growth model, indicating sustained expansion of the field. China and the United States were the most productive countries/regions, whereas Belgium and Poland showed the highest citation impact among leading contributors. The University of Wrocław and the Russian Academy of Sciences emerged as prominent institutional hubs. Keyword co-occurrence analysis identified three major thematic directions: phage discovery and genomic characterization, antibacterial and antibiofilm applications, and mechanistic or structural studies of depolymerase activity, with Klebsiella pneumoniae being the most frequently studied bacterial target. Conclusion: This bibliometric analysis systematically maps the 16-year landscape of phage depolymerase-enabled anti-infective research, delineating the leading forces and thematic evolution that have shaped the field. These trends highlight promising avenues for mechanistic studies on the structural basis of depolymerase substrate specificity and preclinical research on formulation, safety, and manufacturing, supporting the translational development of depolymerase-related anti-infectives.

In diabetic wounds, diverse reactive oxygen species (ROS) intertwine to form a complex oxidative stress network, directly causing damage to cells and tissues. Most single-therapy materials fail to fully regulate this complex oxidative stress microenvironment, hindering wound healing. In this study, a nanozyme-hydrogel composite system was constructed. Through the complementarity of photothermal and enzyme-catalytic functions, cellular functions were enhanced while the oxidative stress microenvironment of diabetic wounds was alleviated, thereby collectively promoting wound healing. A cerium oxide (CeO2)-based nanozyme with abundant oxygen vacancies and high photothermal efficiency was fabricated via gadolinium (Gd) doping and platinum (Pt) nanocluster modification. Gd doping enhanced the catalase (CAT) -mimetic activity by increasing oxygen vacancies, while Pt nanoclusters enabled photothermal conversion. Through this functional complementarity, efficient wound regulation was achieved. To ensure stable retention, the nanozyme (GCP) was loaded into an in situ photo-cross-linkable hydrogel to form the GCP@PG dressing. In vitro and in vivo, GCP@PG scavenged intracellular ROS and promoted angiogenesis. In diabetic rats with full-thickness skin defects, the wound closure rate reached 97.95% on day 14. By integrating the functional complementarity of enzyme catalysis and photothermal effects, this composite system provides a strategy for diabetic wound repair.

Candida albicans (C. albicans) is a major opportunistic fungal pathogen that causes life-threatening infections, particularly in immunocompromised individuals, underscoring the critical need to understand its pathogenic mechanisms. This study investigates the role of Cas5, a key transcription factor, in regulating C. albicans cell wall remodeling, virulence, and host interactions. Genetic manipulation and biochemical assays were used to examine the effects of Cas5 depletion on C. albicans cell wall structure, adhesion to host cells, morphology transition, innate immune cells recruitment, and pathogenicity in a BALB/C mouse model of oropharyngeal candidiasis (OPC). The results showed that the Cas5 depletion mediated β-glucan exposure and enhanced C. albicans’s ability to recruit neutrophils in vivo. Additionally, Cas5-mediated changes in cell surface hydrophobicity (CSH), CWP expressions, and morphological transition promoted C. albicans adhesion to biologically active surfaces (host cells) and increased fungal burden in the mouse model of OPC. In conclusion, Cas5 modulates C. albicans cell wall remodeling by masking cell wall β-glucan, altering CSH, and regulating the expression of cell wall proteins (CWPs). Additionally, Cas5 participates in inhibiting neutrophil recruitment and enhancing the C. albicans adhesion to host cells, as well as facilitating morphological transitions. These actions promote the colonization and invasion of C. albicans in OPC pathogenesis.

Histone deacetylation affects Candida albicans ( C. albicans ) pathogenicity by modulating virulence factor expression and DNA damage. The histone deacetylase Sir2 is associated with C. albicans plasticity and maintains genome stability to help C. albicans adapt to various environmental niches. However, whether Sir2-mediated chromatin modification affects C. albicans virulence is unclear. The purpose of our study was to investigate the effect of Sir2 on C. albicans pathogenicity and regulation. Here, we report that Sir2 is required for C. albicans pathogenicity, as its deletion affects the survival rate, fungal burden in different organs and the extent of tissue damage in a mouse model of disseminated candidiasis. We evaluated the impact of Sir2 on C. albicans virulence factors and revealed that the Sir2 null mutant had an impaired ability to adhere to host cells and was more easily recognized by the innate immune system. Comprehensive analysis revealed that the disruption of C. albicans adhesion was due to a decrease in cell surface hydrophobicity rather than the differential expression of adhesion genes on the cell wall. In addition, Sir2 affects the distribution and exposure of mannan and β-glucan on the cell wall, indicating that Sir2 plays a role in preventing the immune system from recognizing C. albicans . Interestingly, our results also indicated that Sir2 helps C. albicans maintain metabolic activity under hypoxic conditions, suggesting that Sir2 contributes to C. albicans colonization at hypoxic sites. In conclusion, our findings provide detailed insights into antifungal targets and a useful foundation for the development of antifungal drugs. IMPORTANCE: Candida albicans ( C. albicans ) is the most common opportunistic fungal pathogen and can cause various superficial infections and even life-threatening systemic infections. To successfully propagate infection, this organism relies on the ability to express virulence-associated factors and escape host immunity. In this study, we demonstrated that the histone deacetylase Sir2 helps C. albicans adhere to host cells and escape host immunity by mediating cell wall remodeling; as a result, C. albicans successfully colonized and invaded the host in vivo . In addition, we found that Sir2 contributes to carbon utilization under hypoxic conditions, suggesting that Sir2 is important for C. albicans survival and the establishment of infection in hypoxic environments. In summary, we investigated the role of Sir2 in regulating C. albicans pathogenicity in detail; these findings provide a potential target for the development of antifungal drugs.

Catheter-related infection (CRI) is a common nosocomial infection caused by candida albicans during catheter implantation. Typically, biofilms are formed on the outer surface of the catheter and lead to disseminated infections, which are fatal to patients. There are no effective prevention and treatment management in clinics. Therefore, it is urgent to establish an animal model of CRI for the preclinical screening of new strategies for its prevention and treatment. In this study, a polyethylene catheter, a widely used medical catheter, was inserted into the back of the BALB/c mice after hair removal. Candida albicans ATCC MYA-2876 (SC5314) expressing enhanced green fluorescent protein was subsequently inoculated on the skin's surface along the catheter. Intense fluorescence was observed on the surface of the catheter under a fluorescent microscope 3 days later. Mature and thick biofilms were found on the surface of the catheter via scanning electron microscopy. These results indicated the adhesion, colonization, and biofilm formation of candida albicans on the surface of the catheter. The hyperplasia of the epidermis and the infiltration of inflammatory cells in the skin specimens indicated the histopathological changes of the CRI-associated skin. To sum up, a mouse CRI model was successfully established. This model is expected to be helpful in the research and development of therapeutic management for candida albicans associated CRI.

Periprosthetic joint infection (PJI) is one of the common infections caused by Candida albicans (C. albicans), which increasingly concerns surgeons and scientists. Generally, biofilms that can shield C. albicans from antibiotics and immune clearance are formed at the infection site. Surgery involving the removal of the infected implant, debridement, antimicrobial treatment, and reimplantation is the gold standard for the treatment of PJI. Thus, establishing animal PJI models is of great significance for the research and development of new drugs or therapeutics for PJI. In this study, a smooth nickel-titanium alloy wire, a widely used implant in orthopedic clinics, was inserted into the femoral joint of a C57BL/6 mouse before the C. albicans were inoculated into the articular cavity along the wire. After 14 days, mature and thick biofilms were observed on the surface of implants under a scanning electronic microscope (SEM). A significantly reduced bone trabecula was found in the H&E staining of the infected joint specimens. To sum up, a mouse PJI model with the advantages of easy operation, high successful rate, high repeatability, and high clinical correlation was established. This is expected to be an important model for clinical studies of C. albicans biofilm-related PJI prevention.

Introduction: The effect of traditional treatment for melanoma is quite limited, especially for its recurrence. As the major components of yeast cell wall, chitin and β-glucan exhibit good immune activation effect and are promising candidates for adjuvant. Therefore, melanoma cell membrane (CM) and indocyanine green (ICG) was loaded in a chitin and β-glucan hybrid hydrogel to achieve an enhanced anti-melanoma therapy. Methods: The novel hybrid hydrogel was prepared, and its physicochemical properties were examined. Its effect towards melanoma prevention and treatment was evaluated via a melanoma-bearing mice model. Results: The CM-ICG-hybrid hydrogel was successfully prepared with excellent injectability, self-healing, drug loading, rheological, in vitro and in vivo photothermal stability, and retention properties. It also exhibited good cellular and in vivo safety profiles. In the primary melanoma mice model, it quickly ablated the in-situ melanoma, effectively inhibited the tumor growth, increased the survival rate of melanoma-bearing mice, and increased the level of IFN-γ and TNF-α. In the distal secondary melanoma model, it efficiently prevented the reoccurrence of melanoma and activated the memory T cells. In both models, a synergistic effect of photothermal therapy and immune therapy was found. The hydrogel effectively recruited CD3⁺ CD4⁺ T cells and CD3⁺ CD8⁺ T cells, inhibited the proliferation of melanoma cells, and induced the apoptosis of melanoma cells. Conclusion: The hybrid hydrogel was successfully prepared, and it showed excellent efficacy towards melanoma prevention and treatment due to its efficient tumor ablation and immune activation capability. Graphical Abstract:

Introduction: Hepatic ischemia-reperfusion injury (HIRI) is the main reason for liver dysfunction or failure after liver resection and liver transplantation. As excess accumulation of reactive oxygen species (ROS) is the leading factor, ceria nanoparticle, a cyclic reversible antioxidant, is an excellent candidate for HIRI. Methods: Manganese doped mesoporous hollow ceria nanoparticles (MnO_x-CeO₂ NPs) were prepared, and the physicochemical characteristics, such as particle size, morphology, microstructure, etc. were elucidated. The in vivo safety and liver targeting effect were examined after i.v. injection. The anti-HIRI was determined by a mouse HIRI model. Results: MnO_x-CeO₂ NPs with 0.40% Mn doped exhibited the strongest ROS-scavenging capability, which may due to the increased specific surface area and surface oxygen concentration. The nanoparticles accumulated in the liver after i.v. injection and exhibited good biocompatibility. In the HIRI mice model, MnO_x-CeO₂ NPs significantly reduced the serum ALT and AST level, decreased the MDA level and increased the SOD level in the liver, prevent pathological damages in the liver. Conclusion: MnO_x-CeO₂ NPs were successfully prepared and it could significantly inhibit the HIRI after i.v. injection. Graphical Abstract:

The Moon is the only celestial body that human beings have visited. The design of the Earth-Moon transfer orbits is a critical issue in lunar exploration missions. In the 21st century, new lunar missions including the construction of the lunar space station, the permanent lunar base, and the Earth-Moon transportation network have been proposed, requiring low-cost, expansive launch windows and a fixed arrival epoch for any launch date within the launch window. The low-energy and low-thrust transfers are promising strategies to satisfy the demands. This review provides a detailed landscape of Earth-Moon transfer trajectory design processes, from the traditional patched conic to the state-of-the-art low-energy and low-thrust methods. Essential mechanisms of the various utilized dynamic models and the characteristics of the different design methods are discussed in hopes of helping readers grasp the basic overview of the current Earth-Moon transfer orbit design methods and a deep academic background is unnecessary for the context understanding.