Chang-Yue Chiang

A label-free biosensor based on a wedge-shaped side-polished fiber Bragg grating (SP-FBG) is experimentally demonstrated for real-time immunoglobulin G (IgG) detection. By precisely side-polishing the fiber cladding and partially exposing the core, the guided core-mode optical field is directly accessible to the surrounding environment, enabling strong interaction with surface-bound configuration. This core-exposed FBG differs from conventional evanescent-field-based FBG sensors and operates in a leakage-dominated regime, where external refractive index variations redistribute the effective refractive index of the grating region. The refractive index (RI) response was first evaluated using sodium chloride (NaCl) solutions, showing a stable and monotonic Bragg wavelength blue shift with increasing bulk RI and an RI sensitivity of approximately 10.6 nm/RIU (R² = 0.972). For immunoassay applications, the polished fiber surface was functionalized via APTES silanization followed by EDC/NHS chemistry to covalently immobilize anti-IgG, with bovine serum albumin (BSA) used for blocking nonspecific binding. Sequential exposure to IgG solutions over a concentration range of 10⁻⁸ to 10⁻⁴ g/mL produced concentration-dependent wavelength shifts, and a linear calibration was obtained using log₁₀[IgG] within the tested range. Following IUPAC signal-to-noise criteria, the baseline noise was experimentally determined from five repeated PBS blank measurements (σ = 0.0015 nm), yielding a limit of detection and quantification were determined to be 1.57 ng/mL and 5.23 ng/mL, respectively. The proposed leakage-dominated SP-FBG architecture combines high RI sensitivity with preserved mechanical robustness and good probe-to-probe reproducibility, providing a practical fiber-optic platform for rapid, label-free biosensing.

This work proposes a carboxyl-graphene-oxide-based fiber optic localized surface plasmon resonance biosensor which employed anti-Cardiac Troponin I (anti-cTnI) as the recognition element to detect the cTnI-protein in 10 min with a limit of detection (LOD) of 5.8 pM, which meets the acceptable LOD for clinical testing of 0.04 ng/mL.

Rapid, sensitive, and reliable detection of high mobility group box 1 (HMGB1) is essential for medical and diagnostic applications due to its important role as a biomarker of chronic inflammation. Here, we report a facile method for the detection of HMGB1 using carboxymethyl dextran (CM-dextran) as a bridge molecule modified on the surface of gold nanoparticles combined with a fiber optic localized surface plasmon resonance (FOLSPR) biosensor. Under optimal conditions, the results showed that the FOLSPR sensor detected HMGB1 with a wide linear range (10⁻¹⁰ to 10⁻⁶ g/mL), fast response (less than 10 min), and a low detection limit of 43.4 pg/mL (1.7 pM) and high correlation coefficient values (>0.9928). Furthermore, the accurate quantification and reliable validation of kinetic binding events measured by the currently working biosensors are comparable to surface plasmon resonance sensing systems, providing new insights into direct biomarker detection for clinical applications.

This study develops a highly sensitive and low-cost carboxyl-graphene-oxide-based planar optical waveguide localized surface plasmon resonance biosensor (GO-OW LSPR biosensor), a system based on measuring light intensity changes. The structure of the sensing chip comprises an optical waveguide (OW)-slide glass and microfluidic-poly (methyl methacrylate) (PMMA) substrate, and the OW-slide glass surface-modified gold nanoparticle (AuNP) combined with graphene oxide (GO). As the GO has an abundant carboxyl group (–COOH), the number of capture molecules can be increased. The refractive index sensing system uses silver-coated reflective film to compare the refractive index sensitivity of the GO-OW LSPR biosensor to increase the refractive index sensitivity. The result shows that the signal variation of the system with the silver-coated reflective film is 1.57 times that of the system without the silver-coated reflective film. The refractive index sensitivity is 5.48 RIU⁻¹ and the sensor resolution is 2.52 ± 0.23 × 10⁻⁶ RIU. The biochemical sensing experiment performs immunoglobulin G (IgG) and streptavidin detection. The limits of detection of the sensor for IgG and streptavidin are calculated to be 23.41 ± 1.54 pg/mL and 5.18 ± 0.50 pg/mL, respectively. The coefficient of variation (CV) of the repeatability experiment (sample numbers = 3) is smaller than 10.6%. In addition, the affinity constants of the sensor for anti-IgG/IgG and biotin/streptavidin are estimated to be 1.06 × 10⁷ M⁻¹ and 7.30 × 10⁹ M⁻¹, respectively. The result shows that the GO-OW LSPR biosensor has good repeatability and very low detection sensitivity. It can be used for detecting low concentrations or small biomolecules in the future.

In this research, a direct, simple and ultrasensitive fiber optic particle plasmon resonance (FOPPR) biosensing platform for immunoglobulin G (IgG) detection was developed using a gold nanoparticle/graphene oxide (AuNP/GO) composite as signal amplification element. To obtain the best analytical performance of the sensor, experimental parameters including the surface concentration of GO on the AuNPs, formation time of the GO, the concentration of the anti-IgG and incubation time of anti-IgG were optimized. The calibration plots displayed a good linear relationship between the sensor response (ΔI/I₀) and the logarithm of the analyte concentrations over a linear range from 1.0 × 10⁻¹⁰ to 1.0 × 10⁻⁶ g/mL of IgG under the optimum conditions. A limit of detection (LOD) of 0.038 ng/mL for IgG was calculated from the standard calibration curve. The plot has a linear relationship (correlation coefficient, R = 0.9990). The analytical performance of present work’s biosensor was better than that of our previously reported mixed self-assembled monolayer of 11-mercaptoundecanoic acid/6-mercapto-1-hexanol (MUA/MCH = 1:4) method by about three orders of magnitude. The achieved good sensitivity may be attributed to the synergistic effect between GO and AuNPs in this study. In addition, GO could immobilize more antibodies due to the abundant carboxylic groups on its surface. Furthermore, we also demonstrated that the results from this sensor have good reproducibility, with coefficients of variation (CVs) < 8% for IgG. Therefore, the present strategy provides a novel and convenient method for chemical and biochemical quantification and determination.

We developed a label-free, real-time, and highly sensitive nucleic acid biosensor based on fiber optic particle plasmon resonance (FOPPR). The biosensor employs a single-strand deoxyoligonucleotides (ssDNA) probe, conjugated to immobilized gold nanoparticles on the core surface of an optical fiber. We explore the steric effects on hybridization affinity and limit of detection (LOD), by using different ssDNA probe designs and surface chemistries, including diluent molecules of different lengths in mixed self-assembled monolayers, ssDNA probes of different oligonucleotide lengths, ssDNA probes in different orientations to accommodate target oligonucleotides with a hybridization region located unevenly in the strand. Based on the optimized ssDNA probe design and surface chemistry, we achieved LOD at sub-nM level, which makes detection of target oligonucleotides as low as 1 fmol possible in the 10-μL sensor chip. Additionally, the FOPPR biosensor shows a good correlation in determining HLA-B27 mRNA, in extracted blood samples from patients with ankylosing spondylitis (AS), with the clinically accepted real-time reverse transcription-polymerase chain reaction (RT-PCR) method. The results from this fundamental study should guide the design of ssDNA probe for anti-sense sensing. Further results through application to HLA-B27 mRNA detection illustrate the feasibility in detecting various nucleic acids of chemical and biological relevance.

A novel multi-microchannel biochip fiber-optic particle plasmon resonance (FOPPR) sensor system for the simultaneous detection of multiple samples. The system integrates a novel photoelectric system, a lock-in module, and an all-in-one platform incorporating optical design and mechanical design together to improve system stability and the sensitivity of the FOPPR sensor. The multi-microchannel FOPPR biochip has been developed by constructing a multi-microchannel flow-cell composed of plastic material to monitor and analyze five samples simultaneously. The sensor system requires only 30 μL of sample for detection in each microchannel. Moreover, the total size of the multi-microchannel FOPPR sensor chip is merely 40 mm × 30 mm × 4 mm; thus, it is very compact and cost-effective. The analysis was based on calibration curves obtained from real-time sensor response data after injection of sucrose solution, streptavidin and anti-dinitrophenyl (anti-DNP) antibody of known concentrations over the chips. The results show that the multi-microchannel FOPPR sensor system not only has good reproducibility (coefficient of variation (CV) < 10%), but also excellent refractive index resolution (6.23 ± 0.10 × 10⁻⁶ refractive index unit (RIU)). The detection limits are 2.92 ± 0.28 × 10⁻⁸ g/mL (0.53 ± 0.01 nM) and 7.48 ± 0.40 × 10⁻⁸ g/mL (0.34 ± 0.002 nM) for streptavidin and anti-DNP antibody, respectively.