Geun-Chang Ryu
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Geun-Chang Ryu has a total of 10 co-authors. Here are the top co-authors by number of shared publications:
- Ara Jo
Jeonnam Institute of Natural Resources Research, Jangheung-gun, Jeollanamdo 59338, Korea
1 shared publication - Chul-Yung Choi
Well-Aging Medicare & City G-LAMP Project Group, Chosun University
1 shared publication - Dool-Ri Oh
Natural Resources Research Laboratory, Green Bio Division, Jeonnam Bio Foundation, Jangheung-gun 59338, Republic of Korea
1 shared publication - Hakjoon Choi
Central R&D Center, B&Tech Co., Ltd., Gwangju 61239, Korea
1 shared publication - Huwon Kang
Jeonnam Institute of Natural Resources Research, Jangheung-gun, Jeollanamdo 59338, Korea
1 shared publication - Jaeyong Kim
Jeonnam Institute of Natural Resources Research, Jangheung-gun, Jeollanamdo 59338, Korea
1 shared publication
Latest Publications
- Open Access
Aqueous Extract of Perilla frutescens var. acuta Relaxes the Ciliary Smooth Muscle by Increasing NO/cGMP Content In Vitro and In Vivo
The leaves of Perilla frutescens var. acuta (PFA) are commonly used as a traditional medicine in Korea, Japan, and China. We previously showed that PFA attenuates eye fatigue by improving visual accommodation through a clinical study. However, detailed mechanisms and chemical compounds have not been studied. In this study, we analyzed the active compounds in an aqueous extract of PFA involved in ciliary muscle relaxation in vitro and in vivo. NMR and MS analyses showed that the PFA extract contained mainly luteolin-7-O-diglucuronide and apigenin-7-O-diglucuronide. The composition after freeze-drying and spray-drying was similar. Freeze-dried PFA (50 µg/mL, 100 µg/mL, and 200 µg/mL) increased nitric oxide and cGMP levels in ciliary muscle cells isolated from the eyes of rats. [Ca2+]i decreased in a dose-dependent manner. Furthermore, Sprague-Dawley rats treated with freeze-dried PFA (200 mg/kg, orally) showed significantly increased cGMP levels compared with the control group and irradiated with white light. Our results suggest that PFA extract has the potential to reduce eye fatigue by relaxing ciliary muscles.
Open AccessAqueous Extract of Perilla frutescens var. acuta Relaxes the Ciliary Smooth Muscle by Increasing NO/cGMP Content In Vitro and In Vivo
The leaves of Perilla frutescens var. acuta (PFA) are commonly used as a traditional medicine in Korea, Japan, and China. We previously showed that PFA attenuates eye fatigue by improving visual accommodation through a clinical study. However, detailed mechanisms and chemical compounds have not been studied. In this study, we analyzed the active compounds in an aqueous extract of PFA involved in ciliary muscle relaxation in vitro and in vivo. NMR and MS analyses showed that the PFA extract contained mainly luteolin-7-O-diglucuronide and apigenin-7-O-diglucuronide. The composition after freeze-drying and spray-drying was similar. Freeze-dried PFA (50 µg/mL, 100 µg/mL, and 200 µg/mL) increased nitric oxide and cGMP levels in ciliary muscle cells isolated from the eyes of rats. [Ca2+]i decreased in a dose-dependent manner. Furthermore, Sprague-Dawley rats treated with freeze-dried PFA (200 mg/kg, orally) showed significantly increased cGMP levels compared with the control group and irradiated with white light. Our results suggest that PFA extract has the potential to reduce eye fatigue by relaxing ciliary muscles.