Tian-Yu Dong
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Tian-Yu Dong has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Chuan-Liang Deng
College of Life Sciences, Henan Normal University, Xinxiang 453007, China
1 shared publication - Long-Dou Lu
Henan Normal University
1 shared publication - Ning-Na Wang
Henan Normal University
1 shared publication - Shao-Jing Wang
Henan Normal University
1 shared publication - Shufen Li
College of Life Sciences, Henan Normal University, Xinxiang 453007, China
1 shared publication - Wu-Jun Gao
Henan Normal University
1 shared publication
Latest Publications
- 3 June 2015
- Published by Springer Nature in Journal of Plant Research
- Vol. 128 (5) , 829-838
Isolation of differentially expressed sex genes in garden asparagus using suppression subtractive hybridization
Garden asparagus (Asparagus officinalis L.) is a dioecious species whose male and female flowers are found in separate unisexual individuals. A region called the M-locus, located on a pair of homomorphic sex chromosomes, controls sexual dimorphism in asparagus. To date, no sex determining gene has been isolated from asparagus. To identify more genes involved in flower development in asparagus, subtractive hybridization library of male flowers in asparagus was constructed by suppression subtraction hybridization. A total of 107 expressed sequence tags (ESTs) were identified. BLASTX analysis showed that the library contained several genes that could be related to flower development. The expression patterns of seven selected genes believed to be involved in the development of asparagus male flower were further analyzed by semi-quantitative or real-time reverse-transcription polymerase chain reaction (RT-PCR). Results showed that AOEST 4-5, AOEST 12-40, and AOEST 13-38 were strongly expressed in the male flower stage, whereas no transcript level of AOEST 13-38 was detected in the female flower stage. The expression levels of AOEST 13-87, AOEST 13-92, AOEST 13-40, and AOEST 18-87 in the male flower stage were also higher than those in the female flower stage, although these transcripts were also expressed in other tissues. The identified genes can provide a strong starting point for further studies on the underlying molecular differences between the male and female flowers of asparagus.
- 3 June 2015
- Published by Springer Nature in Journal of Plant Research
- Vol. 128 (5) , 829-838
Isolation of differentially expressed sex genes in garden asparagus using suppression subtractive hybridization
Garden asparagus (Asparagus officinalis L.) is a dioecious species whose male and female flowers are found in separate unisexual individuals. A region called the M-locus, located on a pair of homomorphic sex chromosomes, controls sexual dimorphism in asparagus. To date, no sex determining gene has been isolated from asparagus. To identify more genes involved in flower development in asparagus, subtractive hybridization library of male flowers in asparagus was constructed by suppression subtraction hybridization. A total of 107 expressed sequence tags (ESTs) were identified. BLASTX analysis showed that the library contained several genes that could be related to flower development. The expression patterns of seven selected genes believed to be involved in the development of asparagus male flower were further analyzed by semi-quantitative or real-time reverse-transcription polymerase chain reaction (RT-PCR). Results showed that AOEST 4-5, AOEST 12-40, and AOEST 13-38 were strongly expressed in the male flower stage, whereas no transcript level of AOEST 13-38 was detected in the female flower stage. The expression levels of AOEST 13-87, AOEST 13-92, AOEST 13-40, and AOEST 18-87 in the male flower stage were also higher than those in the female flower stage, although these transcripts were also expressed in other tissues. The identified genes can provide a strong starting point for further studies on the underlying molecular differences between the male and female flowers of asparagus.