Elena Naumovska
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Elena Naumovska has a total of 8 co-authors. Here are the top co-authors by number of shared publications:
- Arnaud Nicolas
Mimetas BV, Model Development, J.H. Oortweg 16, 2333 CH Leiden, The Netherlands
1 shared publication - Christian Wong Valencia
Department of Biomedical Sciences, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
1 shared publication - Dorota Kurek
Mimetas BV, Leiden, The Netherlands
1 shared publication - Germaine Aalderink
Division of Toxicology, Wageningen University and Research, Wageningen, The Netherlands
1 shared publication - Kai Sven Erdmann
School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK
1 shared publication - Kinga Kosim
Mimetas BV, Model Development, J.H. Oortweg 16, 2333 CH Leiden, The Netherlands
1 shared publication
Latest Publications
- Open Access
- 14 July 2020
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (14) , 4964
Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells
Intestinal organoids have emerged as the new paradigm for modelling the healthy and diseased intestine with patient-relevant properties. In this study, we show directed differentiation of induced pluripotent stem cells towards intestinal-like phenotype within a microfluidic device. iPSCs are cultured against a gel in microfluidic chips of the OrganoPlate, in which they undergo stepwise differentiation. Cells form a tubular structure, lose their stem cell markers and start expressing mature intestinal markers, including markers for Paneth cells, enterocytes and neuroendocrine cells. Tubes develop barrier properties as confirmed by transepithelial electrical resistance (TEER). Lastly, we show that tubules respond to pro-inflammatory cytokine triggers. The whole procedure for differentiation lasts 14 days, making it an efficient process to make patient-specific organoid tubules. We anticipate the usage of the platform for disease modelling and drug candidate screening.
- 14 July 2020
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 21 (14) , 4964
Open AccessDirect On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells
Intestinal organoids have emerged as the new paradigm for modelling the healthy and diseased intestine with patient-relevant properties. In this study, we show directed differentiation of induced pluripotent stem cells towards intestinal-like phenotype within a microfluidic device. iPSCs are cultured against a gel in microfluidic chips of the OrganoPlate, in which they undergo stepwise differentiation. Cells form a tubular structure, lose their stem cell markers and start expressing mature intestinal markers, including markers for Paneth cells, enterocytes and neuroendocrine cells. Tubes develop barrier properties as confirmed by transepithelial electrical resistance (TEER). Lastly, we show that tubules respond to pro-inflammatory cytokine triggers. The whole procedure for differentiation lasts 14 days, making it an efficient process to make patient-specific organoid tubules. We anticipate the usage of the platform for disease modelling and drug candidate screening.