Mustafa Karapirli
Research Subjects
Research Interests
Mustafa Karapirli has a total of 13 co-authors. Here are the top co-authors by number of shared publications:
- Emin Ozgur Akgul
Department of Clinical Chemistry, Gulhane School Of Medicine, Ankara, Turkey
1 shared publication Enis Macit
Department of Pharmacology, Gulhane Military Medical Academy, 06018 Ankara, Turkey
1 shared publicationErdinc Cakir
Department of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, Turkey
1 shared publicationHakan Yaren
Department of Medical Chemical/Biological/Radiological/Nuclear Defense, Gulhane Military Medical Academy, Etlik, 06018 Ankara, Turkey
1 shared publicationHalil Yaman
Department of Clinical Biochemistry, Gulhane Military Medical Academy, 06018 Ankara, Turkey
1 shared publication- Husamettin Gul
Gülhane Military Medical Academy
1 shared publication
Latest Publications
- Open Access
- 1 January 2012
- Published by Wiley in The Scientific World Journal
- Vol. 2012, 1-8
Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS
Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples.Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients.Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes.Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs.
- 1 January 2012
- Published by Wiley in The Scientific World Journal
- Vol. 2012, 1-8
Open AccessSimultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS
Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples.Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients.Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes.Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs.