Thilly J. Mbira
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Thilly J. Mbira has a total of 7 co-authors. Here are the top co-authors by number of shared publications:
- Ahmed Abdelfattah Hussein
Chemistry Department, Cape Peninsula University of Technology, Symphony Rd., Bellville 7535, South Africa
1 shared publication - Brendon Pearce
Genetics Department, Faculty of Agriscience, Stellenbosch University, Stellenbosch, South Africa
1 shared publication - Emmanuel Iheanyichukwu Iwuoha
SensorLab (University of the Western Cape Sensor Laboratories) 4th Floor Chemical Sciences Building University of the Western Cape Bellville Cape Town South Africa
1 shared publication - Kadidiatou O. Ndjoubi
Chemistry Department, Cape Peninsula University of Technology, Bellville Campus, Symphony Road, Bellville 7535, South Africa
1 shared publication - Keenau Pearce
Precision Medicine Laboratory, Department of Biotechnology, 2nd Floor, Life Science Building, University of the Western Cape, Cape Town 7530, South Africa
1 shared publication - Mongi Benjeddou
Department of Biotechnology, Faculty of Natural Science, University of the Western Cape, Cape Town 7535, South Africa
1 shared publication
Latest Publications
- Open Access
Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic β-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely, Salvia africana-lutea, Leonotis ocymifolia, and Plectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds 1 (p = 0.0031), 8 (p = 0.0053), and 6 (p = 0.0086) showed a marked increase in glucose uptake, respectively. Additionally, 1, 4, and 6 exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of ~70%, ~68%, and ~67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes.
Open AccessGlucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic β-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely, Salvia africana-lutea, Leonotis ocymifolia, and Plectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds 1 (p = 0.0031), 8 (p = 0.0053), and 6 (p = 0.0086) showed a marked increase in glucose uptake, respectively. Additionally, 1, 4, and 6 exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of ~70%, ~68%, and ~67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes.