Xin-Yue Zhang
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
Xin-Yue Zhang has a total of 6 co-authors. Here are the top co-authors by number of shared publications:
- Feng-Biao Wang
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China
1 shared publication - Hai-Ying Wang
Key Laboratory of Sustainable, Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
1 shared publication - Hao-Dong Ni
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China
1 shared publication - Jie Li
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China
1 shared publication - Yan Ma
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
1 shared publication - Zhi-Peng Wang
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
1 shared publication
Latest Publications
- Open Access
- 9 August 2020
- Published by MDPI AG in Marine Drugs
- Vol. 18 (8) , 416
Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.
- 9 August 2020
- Published by MDPI AG in Marine Drugs
- Vol. 18 (8) , 416
Open AccessCharacterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties
Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.