Ghsaq Alhamdani

Periodontal disease is a bacterial infection leading to the progressive destruction of soft and hard oral tissues. Current clinical treatment involves scaling and root planing (SRP), combined with adjunctive therapies, such as the application of antibacterial reagents. Mānuka oil, derived from Leptospermum scoparium , a native plant in New Zealand and Australia, exhibits broad-spectrum antibacterial activity mainly attributed to its β-triketone compounds. However, due to the limitations, including low solubility and strong volatility, clinical translation of mānuka oil and β-triketones is limited. Given the biocompatible, antimicrobial, and mucoadhesive properties of chitosan, this study aimed to synthesize mānuka oil and β-triketone-loaded chitosan nanoparticles to achieve sustained release and enhanced antimicrobial efficacy, supporting their therapeutic potential as a natural and antibiotic-free alternative for treating bacterial infections, especially in periodontal disease. Mānuka oil and β-triketone-loaded chitosan nanoparticles were synthesized using a microfluidic platform with the average size of 253.0 nm (polydispersity index (PDI): 0.421) and 196.5 nm (PDI: 0.446), respectively. Encapsulation techniques enabled prolonged oil release over 24 h, with effective antimicrobial activity against Staphylococcus aureus , Streptococcus mutans , Aggregatibacter actinomycetemcomitans , and Fusobacterium nucleatum , and good in vitro biocompatibility with human mesenchymal stem cells. Overall, the presented study highlights a phytotherapy using nanoparticles for the local delivery of mānuka oil and β-triketones. These antimicrobial formulations provide potential as an adjunct to SRP in periodontal treatment and broader bacterial infections. Key points: • Periodontal disease arises from the accumulation of dental plaque. • Mānuka oil and its β-triketone compounds exhibit strong antimicrobial activity. • Microfluidics-produced nanoparticles show potential as antibiotic-free adjuncts. Graphical (Created by BioRender).

Periodontal diseases are local bacterial infections that cause inflammation in periodontal (gum) tissues, significantly impacting a patient’s quality of life. Current clinical treatment, such as scaling and root planing combined with antibiotics, shows drawbacks, including antibiotic resistance. The potential of plant-derived bioactives with antimicrobial properties has led to growing interest in the biomedical field. Mānuka oil, an essential oil derived from the Leptospermum scoparium plant, is a potential candidate with known antibacterial and anti-inflammatory properties. Formulation of natural oils for delivery, sustained release, and substantivity in the oral environment is challenging. The integration of nanoencapsulation technology offers the potential to prolong the release time, as well as enhance biocompatibility and address the current therapeutic requirements. Microfluidics enables precise nanoparticle synthesis, while chitosan, due to its antimicrobial activity, muco-adhesion, and biocompatibility, represents a promising encapsulation polymer. This paper aims to review antimicrobial essential oils and nanoencapsulation methods, highlighting the application of microfluidics in developing mānuka oil-loaded chitosan nanoparticles for local delivery in periodontal treatment.

Objective: To investigate the prevalence of maxillary canine impaction and whether the impacted canine cases have had a potentially good or poor prognosis if interceptive treatment was practiced at earlier ages. Material and Methods: 1755 panoramic radiographs of patients, aged 13 to 60 years, attending the University of Babylon, College of Dentistry for the period from November 2016 to July 2018 were collected and assessed for the presence of impacted maxillary canines. Subsequently, four suggested prognostic factors for each impacted canine were analyzed and recorded. Results: The prevalence of maxillary canine impaction was 119 (6.78%) with 89 unilateral impaction and 30 bilateral impaction resulting in a total of 149 impacted canines. Regarding the gender, impaction was more evident in females 70 than in males 49 with no statistical significant difference. For the side, impaction was more prevalent on the left (77) than on the right (72), also with no statistical difference (p>0.05). Following the analysis of the four prognostic factors, most of the cases, 117 (78.5%) had at least one prognostic factor to be poor. Although 82 (55%) of the cases had at least one prognostic factor to be good, only 4 had all the prognostic factors to be good. Conclusion: Although the cases that had a potentially good prognosis were little (4 cases), interceptive extraction of primary canines would mostly be beneficial for these patients as it will minimize the treatment time, cost and complications.