Greg Underwood

Infectious laryngotracheitis virus (ILTV) remains a significant viral disease in the poultry industry worldwide and vaccination has proven to be an invaluable tool for disease control. Vaccine type, dose and route of administration are important parameters that determine the success of vaccination programmes and control strategies. The current study aimed to investigate the optimal dose for drinking-water vaccination with ΔgG-ILTV, an attenuated glycoprotein G-deficient ILTV vaccine that is efficacious when administered by eye-drop. Three groups of one-week old specific-pathogen-free chickens were vaccinated with increasing doses of ΔgG-ILTV (10^3.8, 10^4.3 and 10^5.0 plaque forming units per bird) via the drinking-water. Additional groups of birds included an eye-drop vaccination control (n = 20), and two unvaccinated control groups (n = 20 and 10, respectively). Three weeks after vaccination, all groups, except one unvaccinated control group (n = 10), were challenged with virulent ILTV. Vaccine efficacy was assessed after challenge by recording mortality rate and scoring of clinical signs and gross tracheal pathology. Challenge resulted in severe clinical disease and a high mortality rate in unvaccinated birds. Eye-drop vaccination resulted in complete clinical protection against this specific challenge. The efficacy of drinking-water vaccination showed a direct association with the administered vaccine dose. Results from this study highlight the need for improved understanding of virus-host interactions and immunological responses that occur following drinking-water vaccination, in order to improve the efficacy of vaccination strategies that use this route.

Mycoplasma gallisepticum is the primary causative agent of chronic respiratory disease in poultry, and vaccination is the measure most commonly used for its control. Pathological changes caused by M. gallisepticum are mainly observed in the trachea and air sacs, but assessment of air sac lesions is subjective. Standardized parameters for evaluation of pathological changes, and their reproducibility and discrimination in uninfected and infected groups, are critical when assessing the efficacy of M. gallisepticum vaccination. This study reviewed and critically appraised the published literature on evaluation of vaccine efficacy against pathological changes caused by M. gallisepticum in poultry in the trachea and air sacs. A search of four electronic databases, with subsequent manual filtering, identified 23 eligible papers published since 1962 describing the assessment of histopathological changes in the trachea using tracheal lesion scores and/or measurement of tracheal mucosal thicknesses and assessment of gross air sac lesions using lesion scores. Measurement of tracheal lesions proved a more reliable and robust method of assessing disease induced by M. gallisepticum when compared to assessment of air sac lesions, highlighting the importance of including assessment of tracheal lesions as the primary outcome variable in vaccine efficacy studies. In addition, this study also identified the necessity for use of a standardized model for evaluation and reporting on M. gallisepticum vaccines to minimize variations between vaccine efficacy studies and to allow direct comparisons between them.

For modern layers to achieve optimum production performance and welfare state, the entire production process needs to be managed to achieve target bodyweight, body composition and flock uniformity. In addition to genetic improvements, flock health, environment, nutrition and on-farm husbandry practices, incubation and hatchery practices have become a focal point for producing optimum chick quality. Chick quality is a collective term involving chick size and anatomical features, physiology, robustness and liveability over the first week after placement. Chick quality is a key focus for hatcheries as this has been positively correlated with overall flock performance, especially egg production, shell quality, liveability and animal welfare. Recent advances in incubation have focussed on the benefits of all-in–all-out (single-stage) machines. Innovation in incubation systems is completely conducted today by international manufacturers, and is largely driven by the meat chicken industry. Disposal of male layer chicks has increased as a consumer welfare concern, and while investment in research and legislation changes are focussed towards providing or driving new solutions, there are currently no commercial options for industry to deal with this problem before incubation or hatch. Chick transport systems and equipment have also significantly improved in recent years, providing optimum conditions to ensure temperature uniformity and minimal moisture loss during transit before placement, thus optimising chick quality. Together, recent improvements in incubation equipment and hatchery practice can produce commercial layer chicks that are healthy, of high quality, and are then in an optimal physiological state and condition to achieve their genetic potential.

Modern commercial layer breeds represent the culmination of ~7000 years of natural genetic selection. This selection was driven in former times by a combination of genetic-shift and -drift events, that led to chickens being favoured as domesticated species for meat and egg production. More recently, in the early 20th century, the concept of hybrid vigour was discovered and accelerated the natural breeding progress that delivered new genetic lines and more favourable production traits. In the mid-20th century, the broiler-type and egg layer-type lines diverged and, in the 21st century, genetic analysis has further accelerated the progress made towards extended primary breeding characteristics such as egg quality, production and feed-intake traits, together with secondary breeding characteristics such as behavioural traits that have improved robustness in different housing systems, climates and feed types, which together have significantly improved welfare traits. Most recently, there has been the adoption of higher-powered computational analytics together with quantitative trait loci and single-nucleotide polymorphism assessment, which have further improved the uniformity of production traits within breeds. Most importantly, this has provided the primary breeding companies with improved and broader basis of selection of the modern commercial layer breeds, which also improved the alignment of layer strains with market requirements, and diverse variations in housing, nutritional and environmental conditions. This is also testament to the speed with which the commercial layer geneticists can respond to changing welfare policy on factors such as stocking density and beak treatment. The present paper reviews the modern approaches to genetic selection, including considerations of and benefits to the welfare state of commercial layers.

Salmonella Typhimurium is among the most common causes of bacterial foodborne gastrointestinal disease in humans. Food items containing raw or undercooked eggs are frequently identified during traceback investigation as the source of the bacteria. Layer hens can become persistently infected with Salmonella Typhimurium and intermittently shed the bacteria over the course of their productive lifetime. Eggs laid in a contaminated environment are at risk of potential exposure to bacteria. Thus, mitigating the bacterial load on farms aids in the protection of the food supply chain. Layer hen producers use a multifaceted approach for reducing Salmonella on farms, including the all-in-all-out management strategy, strict biosecurity, sanitization, and vaccination. The use of live attenuated Salmonella vaccines is favored because they elicit a broader host immune response than killed or inactivated vaccines that have been demonstrated to provide cross-protection against multiple serovars. Depending on the vaccine, two to three doses of Salmonella Typhimurium vaccines are generally administered to layer hens within the first few weeks. The productive life of a layer hen, however, can exceed 70 weeks and it is unclear whether current vaccination regimens are effective for that extended period. The objective of this review is to highlight layer hen specific challenges that may affect vaccine efficacy.

Beak trimming is used in the egg industry to prevent mortality from cannibalism and minimise injurious pecking, vent pecking, aggressive pecking at the head and all forms of feather pecking, although the practice does not completely reduce the damage. There are alternatives to beak trimming, but they have not been reliable in preventing injurious pecking. However, beak trimming should not be used without providing birds enriched indoor and outdoor environments. Even when birds are beak trimmed, providing enriched facilities is recommended. The two main methods of beak trimming are hot blade (HB) and infrared beak treatment (IRBT). HB trimming removes the beak tips and cauterises the beak stump. The IRBT method uses heat from an infrared lamp to treat the outer beak and the underlying tissue. Initially, the tips of the beak remain intact and then soften and wear as the bird uses its beak. In contrast, the HB method results in an open wound that takes 3 weeks to heal. Two major welfare issues arise from beak trimming. The first is loss of sensory input because of removal of or heat treatment of sensory receptors in the beak. The second issue is the potential for acute and chronic pain from severing or heat treatment of nerves. HB trimming initially results in acute pain but there is limited evidence for beak sensitivity in IRBT birds. The development of neuromas in the beak have been implicated as a cause of chronic pain after HB trimming. When birds are HB trimmed (one-half of upper beak; one-third of lower) in the first 10 days of life, neuromas will develop, but they will resolve, compared with birds trimmed at older ages. However, neuromas will not resolve in 10-day old birds if more than one-half of the beak is HB trimmed. While HB trimming is performed according to accreditation standards by removal of one-half the upper beak and one-third of the lower beak is considered excessive, it prevents beak regrowth and the need to subject birds to re-trimming and a second bout of acute pain from the beak wound. Current levels of IRBT to treat half of the beak using the vendors quality-assurance protocol have been implemented worldwide to ensure that neuroma formation is prevented; however, if severe levels of IRBT are used, acute pain and subsequent neuromas may persist. The main impact of beak trimming is how the bird uses its beak when it eats, drinks and pecks at other birds and the environment. Reduced feed intake after HB trimming indicates pain associated with pecking or difficulty in eating. HB-trimmed birds initially show an increase in listlessness and guarding behaviours and increased inactivity, which indicates pain. IRBT and HB-trimmed birds have fewer aggressive pecks at the head, and there is a reduction in severe feather pecking and better feather scores, which results in a large reduction in mortality. Depending on the severity of IRBT and HB trimming, the advantage of using IRBT is improved welfare.

Salmonella vaccination is one of the control measure that farmers can use to reduce bacterial shedding in their flocks. This study aimed to examine the efficacy of the Vaxsafe® ST (Strain STM-1) attenuated live vaccine administered as ocular and oral doses followed by an intramuscular (IM) dose in rearing, in reducing contamination by Salmonellae of both eggs and the environment in the commercial multi-age cage layer sheds. A randomised controlled trial was conducted up to 26 weeks post last vaccine on two different multi-age caged egg farms. No clinical symptoms were observed following IM administration of STM-1 during rearing. Following the first two STM-1 doses, both vaccinated and unvaccinated birds exhibited antibody titres below the positive cut-off value, however after IM administration of STM-1, antibody titres in the vaccinated group were above the cut-off value. Wild type Salmonella Typhimurium was not detected during the rearing of pullets. During production, the antibody titres were significantly higher in the vaccinated group at all sampling points during this trial. There was no significant difference in the prevalence of Salmonella (detected by culture and PCR method) between the vaccinated and unvaccinated groups on the egg belt and faeces in early lay. Wild-type Salmonella spp. were consistently found in dust samples. Quantitative PCR (qPCR) assay was able to differentiate between the live vaccine strain and wild type Salmonella. The load of wild-type Salmonella in shed environment was relatively low (1.3 log₁₀ ± 0.48 CFU/m² of surface area). Given that Salmonella Typhimurium and other serovars are able to survive/persist in the shed environment (such as in dust), regular cleaning and or removal of dust from shed is important. Use of the Vaxsafe® ST vaccine in multi-age flocks is “not an ultimate intervention” for reduction of Salmonella Typhimurium because of the complexities involved in achieving control, such as the efficacy of cleaning of sheds, the lack of resting periods between batches and the possible carry over of contamination from existing flocks. Hence implementation of more than one or several interventions strategies is essential.