Ellen de Souza Marquez

This study aimed to evaluate the effects of autohemotherapy as an adjuvant in the control of gastrointestinal nematodes in sheep. Four experimental groups were formed: G1, 10 animals receiving autologous venous blood; G2, 10 animals receiving autologous venous blood and vermifuge containing levamisole; G3, 10 animals receiving only vermifuge containing levamisole; and G4, 10 animals as the control group receiving no treatment. We performed fecal egg count (eggs per gram, EPG) of strongyles, larval culture, hemogram, leukogram, and serum protein dosage prior to the start of treatment (D0), and on days 14 (D14) and 42 (D42). There was a significant decrease in the EPG of the groups receiving levamisole (G2 and G3) from D14 to the end of the experimental period. At the end of the evaluations, the mean EPG of G2 and G3 was significantly lower than that of G1 and G4. The most common nematode genus was Haemonchus (88%), and the least common was Trichostrongylus (1%). The Fecal Egg Count Reduction Test (FECRT) of G2 and G3 on D14 were 98.1% and 97.9%, respectively, however, in G1, the FECRT was zero on the two days when evaluation took place. G1 and G2 showed a significant increase in monocyte counts on D14 and D42. There was a significant increase in hematocrit and hemoglobin values in G2 and G3, however, a significant increase in the absolute value of red blood cells was observed only in G2. Two doses of autohemotherapy at 21-day intervals, administered alone or as an adjuvant to levamisole, is ineffective in controlling gastrointestinal nematodes in naturally infected sheep.

Glucose is an essential carbohydrate for the good functioning of the organism and in normal conditions it is able to maintain the glucose in adequate levels. The glucose can be measured by the portable glucometer or by the laboratory method, which is considered the standard methodology, but with disadvantages such as cost and the necessary amount of blood. The portable glucometer shows itself to be an applicable option because it is less expensive and faster. This prospective clinical study aimed to compare and evaluate the efficiency between the OneTouch UltraMini® portable monitor and the automated colorimetric method for measuring the blood glucose concentration of 48 dogs and 20 cats. The results obtained from the mean of the portable monitor and laboratory method were 87.54 mg/dL and 94.47 mg/dL for dogs, and 85.95 mg/dL and 100 mg/dL for cats, respectively. It could be concluded that the comparison between the methods showed that the portable meter represents an excellent option for measuring blood glucose in dogs and cats.

In an attempt to verify the points of convergence between One Health, Integrative Community Therapy (ICT) and aspects of the COVID-19 Pandemic, a critical essay was proposed, through a qualitative approach that aimed to bring an interdisciplinary, interprofessional and intersectoral reading on the subject. To better understand the concepts and practical aspects of One Health and ICT, it was proposed the adaptation of One Health Umbrella’s figure, with elements of the ICT. The approach of One Health and ICT in face of globalization, the aspects of its complexity and the individual and collective empowerment to reach the administrative spheres, both in the elaboration of the planning and in the definition of the protocols that, as a rule, touch the citizen's skin. Therefore, One Health and ICT are articulated around a unique and fraternal vision of life.

Although anemia has been historically linked to Haemonchus contortus infection, other infectious agents, such as hemotropic mycoplasmas and tick-borne disease pathogens, may also lead to anemic crisis in sheep. This study has aimed to investigate infections related to anemia in a sheep herd from Bandeirantes City, Paraná State, southern Brazil. Seven out of forty-two (16.6%; 95% CI: 8.32–30.6%) sheep were positive for hemoplasmas by a PCR targeting the 16S rRNA gene and all tested negative for A. marginale/A. ovis and Babesia/Theileria spp. by PCR based on msp4 and 18S rRNA genes, respectively. Two (4.7%; 95% CI: 1.32–15.79%) animals were infested with Rhipicephalus microplus ticks. Fecal egg counting was performed in 38 sheep and 24 (63.15%; 95% CI: 47.2–76.6%) presented > 500 eggs per gram. Phylogenetic analysis of partial sequences of the detected hemotropic Mycoplasma sp. 16S and 23S rRNA genes confirmed that the animals were infected with Mycoplasma ovis. Polymorphism analysis of partial 16S rRNA sequences showed three different genotypes of M. ovis infecting sheep assessed in the present study. Mycoplasma ovis and gastrointestinal nematodes occurs in sheep from the northern region of Paraná State.