Elpiniki Skoufogianni

Agroecosystems must maintain high productivity over time and contribute to restoring the biodiversity and functionality of soils while agroecosystems yield the food we eat; however, the diversity related to food and agriculture has been shrinking. With this systematic review, the narrative and evidence map synthesized existing evidence about how cereal-legume rotations (as a form of diversifying crop diversity) could improve the diversity and function of the plant and functional aspects of biodiversity while restoring the soil health and agroecosystem resilience. A PRISMA 2020 report has been created alongside this work. This evidence will be used to understand improvements in soil physical and biological traits, nutrient cycles, and biologically fixed N, regulated pests/diseases/weeds, productivity and yield stability, environmental efficiency, and outcomes. In addition, several pieces of evidence were included and explained concerning the N cycle in cereal-legume rotations. When used compared to monoculture cereal systems, cereal-legume rotations lead to improved soil structure, activity, and nutritional status (N fixing) and may decrease pests and disease; these conditions often promote a better harvest or lead to higher and/or more stable productivity. Crop residue-based SOC increases are generally moderate in duration and degree. The increase in microbial biomass occurred more quickly over the years. For the environment, cereal-legume rotations generally achieve a lower total environmental efficiency due to lower N fertilizer inputs (N fixing), which means a lower C footprint per ton of production of crops, yet this strategy can also cause some environmental consequences, such as increasing N₂O emissions (due to over N fixing), which cause global warming and nitrate leaching when N is fixed in excess, not coupled with crop requirements, creating pollution. The rotation is context-dependent, so each site-specific system needs to be analyzed to improve trade-offs to yield, productivity, and environmental conservation.

Greece lies within the Mediterranean global biodiversity hotspot and harbors exceptional plant richness and endemism, including numerous medicinal and aromatic plants (MAPs). These taxa underpin long ethnobotanical traditions and contemporary bioeconomy niches (culinary herbs, essential oils, phototherapeutics). The aim of this review is to map (i) the biodiversity knowledge base for Greek MAPs, (ii) recent ethnobotanical evidence, and (iii) sustainability pathways (conservation, cultivation, value chains, and regulation) in a Mediterranean context. The information is presented and analyzed in a critical manner. A total of 148 research studies were systematically reviewed based on the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. Key findings highlight that (i) Greece is a regional plant-diversity hotspot with many MAP endemics in Lamiaceae; (ii) contemporary ethnobotanical knowledge persists and adapts; and (iii) strong sustainability levers exist through Natura 2000 coverage, Good Agricultural and Collection Practice (GACP) and European Medicines Agency (EMA) frameworks, and Protected Designation of Origin (PDO)/United Nations Educational, Scientific and Cultural Organization (UNESCO) recognitions (e.g., Krokos Kozanis saffron, Chios mastic), although threats from climate and land-use change remain significant. In conclusion, Greek MAPs combine high biodiversity value, living ethnobotanical traditions, and tangible bioeconomic opportunities. Their sustainable prospects depend on integrating habitat protection, GACP and FairWild Standard (FairWild)-aligned wild collection and cultivation, domestication of priority endemics, and climate-resilience planning, all supported by traceable value chains and Access and Benefit-Sharing (ABS)/Nagoya Protocol compliance. The review concludes with practical recommendations and a prioritized list of flagship taxa for “conservation through use.”

Sustainable fertilization strategies are increasingly required to enhance crop performance while reducing dependence on synthetic inaputs. In this study, the effectiveness of sea-derived organic amendments, Posidonia oceanica compost and mussel shell powder, was evaluated in Salvia officinalis (sage) cultivation. A pot experiment was conducted in Istron Kalou Xoriou (Crete), using three nitrogen rates (0, 40 and 80 kg ha⁻¹) in combination with four rates of mussel shell powder (0, 50, 100 and 200 g/pot). A total of 9 treatments were set up, each replicated 3 times, resulting in 27 pots. Growth parameters (plant height, total and leaf fresh-dry weight), nitrogen content in plant tissues, nitrogen uptake, and nitrogen use efficiency (NUE) were assessed across three harvest periods. The results indicated that both P. oceanica compost and mussel shell amendments significantly improved soil properties and plant performance. The treatment receiving 200 g/pot of mussel shell powder combined with 80 kg ha⁻¹ fertilization (PH200) consistently produced the highest values for biomass (223.99–383.58 g/plant), nitrogen plant concentration (1.967–2.117%), and nitrogen uptake (1.762–3.248 g/plant). The application of mussel shells effectively increased soil pH, thereby enhancing nutrient availability and promoting nitrogen assimilation. Furthermore, NUE values showed a progressive increase with rising amendments rates. Overall, sea-derived organic amendments demonstrated strong potential as sustainable fertilization materials, contributing to sage productivity improvement while supporting circular management of coastal waste resources.

The growing negative environmental effects associated with chemical fertilizers have led to the promotion of organic fertilizers in agriculture. The purpose of this study was to evaluate the impacts of organic fertilization on nitrogen and phosphorus content, uptake and use efficiency in Origanum dictamnus (Dittany) cultivation. With this aim, a randomized complete blocks field experiment was carried out in Istron Kalou Xoriou (Agios Nikolaos—Crete). The study included three fertilization treatments (N0: 0 kg/ha⁻¹, N1: 1250 kg/ha⁻¹ and N2: 2500 kg/ha⁻¹). Throughout the growing period, measurements were taken for the plant’s content, uptake and efficiency indices of total nitrogen (TN) and phosphorus (P). The findings indicated that the fertilization doses affect nutrient uptake and efficiency. The highest values of TN and P were recorded 60 days after transplants. N1 treatment showed the greatest improvement in nitrogen use efficiency, while phosphorus use efficiency reached its maximum level under N2 treatment. That research can contribute to achieving an in-depth insight of organic fertilization practices for aromatic and medicinal plants such as Dittany, promoting a sustainable agricultural strategy and enhancing product quality.

The urban zone around the city of Volos, a Greek city with a historically industrialized profile, faces threats arising from Potential Toxic Element (PTE) contamination. The scope of this study is to determine the contamination levels of 10 PTEs in three urban areas which are located near the industrial zone in the city of Volos. For this purpose, a total of 30 soil samples from parks, playgrounds and roadsides were collected from the Agios Georgios, Velestino and Rizomilos areas (Magnesia, Central Greece). The sampling was conducted in June 2022 and the concentrations of chromium (Cr), nickel (Ni), copper (Cu), arsenic (As), cadmium (Cd), lead (Pb), iron (Fe), manganese (Mn), cobalt (Co) and zinc (Zn) were measured through inductively coupled plasma mass spectrometry (ICP-MS). The Contamination Factor (CF), Pollution Load Index (PLI) and Geo-accumulation Index (Igeo) revealed moderate pollution in most cases, whereas in some sites the contamination was significant for Ni or for As. Principal Component Analysis showed concomitant changes for some PTEs in Component 1 and for others in Component 2, explaining approximately 67% of the variation. K-means Cluster Analysis showed two distinct groups of PTE-impacted sites within these urban areas. It can be postulated that industrial activities may have a carry-over effect on the soil in residential areas. Frequent monitoring of areas deemed as “contaminated” and time-series data are needed to examine in depth the soil pollution in cities and its possible shifts in relation to the changes in industrialization status in the extended urban areas.

Medicinal and aromatic plants are a consistent component of the biodiversity heritage in numerous countries worldwide. Origanum dictamnus L. (Lamiaceae family), also known as Dittany, an endemic plant of the Greek island of Crete, has been widely used as traditional medicine since antiquity, all over Europe. The aim of the present review is to provide a thorough and detailed account of Dittany in antiquity, the plant’s physical characteristics and ecology, and its cultivation methods, as well as its chemical components, biological properties, and pharmacological studies. The information is presented and analyzed in a critical manner. A total of 86 research studies were systematically reviewed based on the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. The findings indicate that Dittany is one of the most important medicinal and aromatic plants, with many uses not only in pharmacology but also in gastronomy. While a large body of literature exists regarding the application of essential oils, the number of publications concerning the plant’s cultivation is rather small. Therefore, the main focus of this review is on the cultivation methods and the significance of cultivating and employing Dittany in Greece and the wider Mediterranean region in the future. Further research on this plant species is warranted since it has significant medicinal, economic, and environmental value.

In recent years, there has been a growing tendency towards using organic fertilizers instead of chemical ones. This study aimed to evaluate the influence of the organic fertilizer, Prima Humica, on agronomic characteristics and herbaceous plant diversity on Origanum dictamnus (Dittany) cultivation. A field experiment was carried out in Kalo Xorio (Lasithi, Crete) and included three different nitrogen organic fertilization schemes: 0 g/plant (T0—unfertilized), 300 g/plot (T1), and 600 g/plot (T2). Plant height, fresh and dry weight, Leaf Area Index (LAI), and plant diversity during the growing season were measured. The results showed that when the T2 treatment was compared with the unfertilized and the T1 fertilizer, the increase in plant height ranged between 9.18% and 40.61%. Moreover, the total fresh and dry weight were positively affected by the T2 treatment. The total fresh and dry weight varied from 111.6 to 239.8 g per plant and from 36.7 to 77.6 g per plant, respectively. Furthermore, LAI was ameliorated using the T2 fertilizer scheme. Concerning plant diversity, a key finding in this study is that the O. dictamnus ecosystem favors herbaceous plant species richness and Shannon’s diversity index. In total, 18 plant species in the O. dictamnus ecosystem were recorded in the study area. The most frequently occurring plants were Anthemis arvensis L. and Piptatherum miliaceum (L.) Coss. Finally, the highest Shannon’s diversity index of herbaceous plants was detected using the T2 fertilizer scheme.

Nowadays, there is an increasing negative environmental effect of using chemical fertilizer. For this reason, the use of biofertilizers is promoted in the agriculture sector. The purpose of this investigation was to carry out an evaluation of the effects of biological fertilizer (biofertilizers are organic materials that can be used to improve soil properties) use on the growth and agronomic characteristics of Aloysia citrodora. To achieve this aim, a two-year randomized complete blocks field experiment was carried out in central Greece. The experiment included four biological fertilizer levels (0, 50, 100 and 150 N kg ha⁻¹) with three replications. Plant height, dry stem, dry leaves, dry total yield and leaf area index (LAI) were measured during the two growing years. The results showed that in the first and second studied years, the maximum plant height and total dry yield were observed in the BF150 treatment. Moreover, LAI was ameliorated by applying the BF100 and BF150 treatments in both studied years. A key finding in this study is that the A. citrodora ecosystem favors herbaceous plant species richness. Also, soil factors (soil organic matter, P and K) promote positive herbaceous plant diversity within the A. citrodora ecosystem. Finally, A. citrodora could be a promising medicinal plant, cultivated under Mediterranean climatic conditions.

The purpose of this investigation was to study the impact of different rates of bio fertilizer on agronomic characteristics and the relations among herbaceous plant diversity and soil properties of Aloysia citrodora in central Greece. For this aim a two – year Completely Randomized Design (CRD) field experiment was carried out in central Greece. The experiments included four biological fertilizer levels, BF0: 0 kg ha-1, BF50: 50 kg ha-1, BF100: 100 kg ha-1 and BF150: 150 kg ha-1 with 3 replication. Plant height, dry stem, dry leaves, dry total yield and Leaf Area Ιndex (LAI) were measured during the two growing years. The results showed that in the 1st studied the maximum plant height was observed in BF150 treatment while the 2nd year the BF150 level provoked a raise of 16.08% and 1.09% compared to BF50 and BF100, respectively. The 1st year the values of total dry yield were better in BF150 application. The 2nd year the total yield increased by 50.27% in BF100 and 50.09% BF150 treatments. Moreover, LAI was ameliorated by applied the BF100 and BF150 treatments both in two studied years. A key finding in this study is that Aloysia citrodora ecosystem favors herbaceous plant species richness. Also, soil factors (soil organic matter, P and K) promote positive herbaceous plant diversity within the Aloysia citrodora ecosystem. Finally, Aloysia citrodora can be a promising medicinal plant, cultivated under the Mediterranean climatic conditions.

Soil erosion is among the biggest problems in the agricultural sector that can affect ecosystems and human societies. A field of 5° slope was selected to study the runoff, soil and nutrient loss as well as crop productivity in different treatments—conventional tillage (CT) vs. no-tillage (NT), plant vs. no plant cover, contour cultivation (CC) vs. perpendicular to the contour cultivation, (PC) under natural rainfall. The experiment was conducted in central Greece in two cultivation periods. In autumn, the field was cultivated with intercropping Triticosecale and Pisum sativum and in spring with sunflower. The total rainfall was 141.4 mm in the 1st year and 311 mm in the 2nd. We found that runoff in the treatment of no tillage with contour cultivation was 85% lower in both years compared to the no tillage-no plant control. Therefore, the contour cultivation-no tillage treatment had a positive effect by decreasing phosphorus and potassium loss from soil: indeed, there was a decrease in P and K by 55% and 62%, respectively, in the NT compared to the CC treatments. We conclude that the NT-CC treatment with plant cover was the most effective in reducing water runoff and soil nutrient loss and increasing yield.