François Beaurain
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Research Interests
François Beaurain has a total of 3 co-authors. Here are the top co-authors by number of shared publications:
- Carmelo Di Primo
Univ. Bordeaux, CNRS, INSERM, ARNA, UMR 5320, U1212, IECB, F-33000 Bordeaux, France
1 shared publication - Jean-Jacques Toulmé
NOVAPTECH
1 shared publication Michel Laguerre
Institut Européen de Chimie et Biologie, 2CNRS UMR 5144, 16 Avenue Pey Berland, F-33607 Pessac Cedex, France and 3INSERM U386, IFR66 Pathologies Infectieuses et Cancers, Université Victor Segalen, 146 Rue Léo Saignat, F-33076 Bordeaux Cedex, France
1 shared publication
Latest Publications
- Open Access
- 15 July 2003
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 31 (14) , 4275-4284
Molecular dynamics reveals the stabilizing role of loop closing residues in kissing interactions: comparison between TAR-TAR* and TAR-aptamer
A RNA aptamer (R06) raised against the trans‐ activation responsive (TAR) element of HIV‐1 was previously shown to generate a loop–loop complex whose stability is strongly dependent on the selected G and A residues closing the aptamer loop. The rationally designed TAR* RNA hairpin with a loop sequence fully complementary to the TAR element, closed by U,A residues, also engages in a loop–loop association with TAR, but with a lower stability compared with the TAR–R06 complex. UV absorption monitored thermal denaturation showed that TAR–TAR*(GA), in which the U,A kissing residues were exchanged for G,A, is as stable as the selected TAR–R06 complex. Consequently, we used the TAR–TAR* structure deduced from NMR studies to model the TAR–R06 complex with either GA, CA or UA loop closing residues. The results of the molecular dynamics trajectories correlate well with the thermal denaturation experiments and show that the increased stability of the GA variant results from an optimized stacking of the bases at the stem–loop junction and from stable interbackbone hydrogen bonds.
- 15 July 2003
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 31 (14) , 4275-4284
Open AccessMolecular dynamics reveals the stabilizing role of loop closing residues in kissing interactions: comparison between TAR-TAR* and TAR-aptamer
A RNA aptamer (R06) raised against the trans‐ activation responsive (TAR) element of HIV‐1 was previously shown to generate a loop–loop complex whose stability is strongly dependent on the selected G and A residues closing the aptamer loop. The rationally designed TAR* RNA hairpin with a loop sequence fully complementary to the TAR element, closed by U,A residues, also engages in a loop–loop association with TAR, but with a lower stability compared with the TAR–R06 complex. UV absorption monitored thermal denaturation showed that TAR–TAR*(GA), in which the U,A kissing residues were exchanged for G,A, is as stable as the selected TAR–R06 complex. Consequently, we used the TAR–TAR* structure deduced from NMR studies to model the TAR–R06 complex with either GA, CA or UA loop closing residues. The results of the molecular dynamics trajectories correlate well with the thermal denaturation experiments and show that the increased stability of the GA variant results from an optimized stacking of the bases at the stem–loop junction and from stable interbackbone hydrogen bonds.