Anelise Christ-Ribeiro

The increasing occurrence of emerging organic contaminants (EOCs) in environmental matrices has intensified the demand for sensitive, selective, and sustainable analytical methods. Sample preparation plays a pivotal role, particularly for trace-level determination in complex matrices. Biochar is a promising sorbent for sample preparation because of its porous structure, tunable surface chemistry, low cost, and potential to be produced from renewable biomass waste. This review examines the use of biochar-based sorbents to extract EOCs from environmental matrices since 2020, with special emphasis on the relationships among precursor biomass, production conditions, surface chemistry, sorption mechanisms, and extraction performance. Agricultural waste was the predominant biomass class, while solid-phase extraction (SPE), magnetic solid-phase extraction (MSPE), and solid-phase microextraction (SPME) were the most frequently reported approaches. Biochar-based extraction has been applied to a wide range of EOCs, with pesticides being the most investigated analyte class. The authors discuss how pyrolysis conditions, activation, surface chemistry, and other parameters affect analyte retention and desorption. The authors compare different extraction approaches in terms of extraction efficiency, precision, and other analytical parameters. Finally, the authors discuss current limitations and research opportunities to guide the development of biochar-based sorbents as sustainable materials for sample preparation and environmental monitoring.

The search for new technologies for the removal of heavy metals has intensified due to the increasing contamination of aquatic environments. In this context, biopolymer-based nanocomposites have stood out in the synthesis of new adsorbent materials. These nanocomposites are considered promising due to their abundance, low toxicity, versatility, and high affinity for metal ions. Therefore, this work presents a comprehensive discussion on the development, properties, and performance of biopolymer-based nanocomposites applied to the removal of heavy metals from aqueous systems. Biopolymers such as chitosan, cellulose, alginate, lignin, and pectin are highlighted due to their functional groups and the possibility of chemical modification and/or incorporation of nanoparticles to improve adsorption capacity. In addition, the main mechanisms involved in the metal adsorption process, such as ion exchange, electrostatic attraction, complexation, and chelation, are discussed together with the most commonly used isotherm and kinetic models to describe adsorption behavior. Furthermore, the potential for reuse of these materials is also described in order to evaluate their stability. Finally, possible processes related to nanoparticle leaching, bioaccumulation, and potential ecological risks are also discussed.

This study aimed to extract oil from skipjack tuna belly (Katsuwonus pelamis) using thermomechanical extraction and supercritical CO₂ extraction (SFE-CO₂). The SFE-CO₂ process was conducted at 25 MPa and 40 °C for 30 min, 1 h, and 3 h. Thermomechanical extraction yielded 88.1% crude oil, with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents of 7.6% and 19.2%, respectively. In the SFE-CO₂ process, the shortest extraction time (25 MPa, 40 °C, 30 min) resulted in the highest DHA (12.6%) concentration, as well as a total polyunsaturated fatty acid (PUFA) content of 22.4%. This behavior is attributed to the shorter CO₂–matrix contact time, which favored the selective extraction of target compounds. Conversely, extending the extraction time to 3 h under the same pressure and temperature led to the highest overall yield (26.4%). These findings demonstrate that SFE-CO₂ is a promising green technology for the valorization of fish processing by-products, enabling selective recovery of high-value omega-3 fatty acids while promoting more sustainable production practices.

This study aimed to extract rice bran oil rich in γ-oryzanol from white (WB) and parboiled rice bran (PB) using ultrasound as a pre-treatment to supercritical fluid extraction (US + SFE), supercritical fluid extraction (SFE), and conventional solvent extraction. PB oil exhibited superior quality compared to WB, with low free fatty acid (FFA) levels and higher γ-oryzanol content. PB oil extracted by US + SFE achieved a yield of 18.2 ± 0.4%, γ-oryzanol content of 1.53 ± 0.19 g 100 g⁻¹, and low FFA content (0.27 ± 0.01%), showing improved oil quality compared to SFE (yield 13.5 ± 0.3%, γ-oryzanol 1.13 ± 0.08%, FFA 0.55 ± 0.01%) and conventional extraction (yield 25.0 ± 1.3%, γ-oryzanol 2.03 ± 0.04%, FFA 1.12 ± 0.01%). The US + SFE oil also showed lower peroxide value (1.7 mEq kg⁻¹) and preserved fatty acid profiles containing palmitic, oleic, and linoleic acids. US induced structural disruption in bran, enhancing oil release. Additionally, chitosan–gelatin nanoemulsions were developed to protect the extracted oil. Formulations exhibited droplet sizes of 119–352 nm, polydispersity indices below 0.3, and zeta potentials from –12.4 to 38.8 mV. Gelatin-based nanoemulsions maintained FFAs at 0.56 ± 0.2% and peroxide values at 4.71 ± 0.2 mEq kg⁻¹ over 90 days, demonstrating superior oxidative stability. These results highlight the potential of US and SFE combined with nanostructured delivery systems to valorize agro-industrial byproducts and develop stable, functional ingredients and drug carrier systems.

The growing demand for aquaculture has driven the search for sustainable practices and utilization of agro-industrial residues. Brown rice bran, an abundant and low-cost by-product, has emerged as a promising raw material. This dissertation aimed to evaluate solid-state fermentation (SSF) of rice bran using the fungus Rhizopus oryzae and the yeast Saccharomyces cerevisiae with the goal of improving its nutritional value for use in diets formulated for zebrafish (Danio rerio). Proximate composition analyses revealed the strong biotransformation potential of Rhizopus oryzae. Fermentation with this fungus resulted in a significant 36.45% increase in protein content, a 51.62% increase in total polyphenols, and a 13.7% reduction in lipid content. In an in vivo experiment, zebrafish fed a diet containing rice bran fermented by R. oryzae showed the best zootechnical performance, with higher weight gain, specific growth rate, and improved feed conversion. Gene expression analysis showed a significant difference only for glut6, which is related to glucose transport. In summary, the fermentation of brown rice bran with Rhizopus oryzae represents an effective strategy to enhance its nutritional profile, establishing it as a viable alternative for the formulation of more sustainable and efficient diets in aquaculture.

Bio-based nanofibers are gaining increasing attention in nanotechnology owing to their high surface area, interconnected porosity, and capacity to incorporate bioactive compounds. Among natural polymers, gelatin is particularly attractive because of its abundance, low cost, biodegradability, and versatile physicochemical properties. When processed by electrospinning, gelatin combines its amphiphilic nature with the structural advantages of nanofibers, enabling efficient interactions with a wide range of molecules. Nevertheless, pure gelatin nanofibers have drawbacks, such as poor mechanical strength and high-water solubility. To address these limitations, strategies including polymer blending, chemical and physical crosslinking, and multilayer biomaterials have been developed, resulting in improved stability, functionality, and application-specific performance. Therefore, this review summarizes recent advances in the fabrication and functionalization of gelatin nanofibers, highlighting how processing parameters and gelatin source influence electrospinning outcomes and fiber properties. Key applications are also discussed, with emphasis on biomedical, food, environmental, and biosensing. Therefore, gelatin nanofibers represent a sustainable and versatile biomaterial with high potential for advanced technological applications.

The Anthropocene, marked by rapid and extensive environmental changes, poses distinct evolutionary pressures and opportunities for species adaptation. Insects, among the most diverse and resilient taxa, exhibit notable dietary plasticity and the ability to convert low-value biomass—such as agro-industrial and urban waste—into usable nutrients. This review explores how these traits serve as adaptive strategies, enabling insects to thrive and expand into novel, human-altered habitats. We examine the evolution of insect nutritional requirements and how alternative diets influence physiological, behavioral, and reproductive traits, ultimately enhancing resilience to anthropogenic stressors. The capacity of insects to metabolize diverse substrates not only supports their role in food security and circular economy initiatives but also provides valuable insights into detoxification pathways and metabolic flexibility in environments rich in xenobiotics. By synthesizing key studies, we highlight the pivotal role insects play in redefining ecosystem functions under human influence. This review underscores the intersection of nutritional and evolutionary biology in understanding insect success in the Anthropocene, emphasizing the importance of nutritional knowledge for both ecological research and applied insect farming systems.

The integration of emulsion gels in 3D food printing has emerged as a promising strategy to enhance both the structural fidelity and functional performance of printed foods. Emulsion gels, composed of proteins, polysaccharides, lipids, and their complexes, can provide tunable rheological and mechanical properties suitable for extrusion and shape retention. This review explores the formulation strategies, including phase behavior (O/W, W/O, and double emulsions); stabilization methods; and post-printing treatments, such as enzymatic, ionic, and thermal crosslinking. Advanced techniques, including ultrasound and high-pressure homogenization, are highlighted for improving gel network formation and retention of active compounds. Functional applications are addressed, with a focus on meat analogs, bioactive delivery systems, and personalized nutrition. Furthermore, the role of the oil content, interfacial engineering, and protein–polysaccharide interactions in improving print precision and post-processing performance is emphasized. Despite notable advances, challenges remain in scalability, regulatory compliance, and optimization of print parameters. The integration of artificial intelligence can also provide promising advances for smart design, predictive modeling, and automation of the 3D food printing workflow.