Stanislava G. Dmitrienko

Adsorption of silver nanoparticles on polymers may affect the processes in which they participate, adjusting the analytical characteristics of methods for the quantitation of various substances. In the present study, a composite material based on silver triangular nanoplates (AgTNPs) and polyurethane foam was proposed for chemical analysis. The prospects of its application for the solid-phase/colorimetric determination of organic thiols were substantiated. It was found that aggregation of AgTNPs upon the action of thiols is manifested by a decrease in the AgTNPs’ localized surface plasmon resonance band and its significant broadening. Spectral changes accompanying the process can be registered using household color-recording devices and even visually. Four thiols differing in their functional groups were tested. It was found that their limits of detection increase in the series cysteamine < 2-mercaptoethanol < cysteine = 3-mercaptopropionic acid and come to 50, 160, 500, and 500 nM, respectively. The applicability of the developed approach was demonstrated during the analysis of pharmaceuticals and food products.

Microfluidic paper-based analytical devices (µPADs) represent one of the promising green analytical strategies for low-cost and simple determination of various analytes. The actual task is the development of such devices for quantitation of antioxidants, e.g., flavonoids. In this paper, possibilities of a novel three-reagent µPAD including silver nitrate, 4-nitrophenyldiazonium tetrafluoroborate, and iron(III) chloride as reagents are assessed with respect to the determination of dihydroquercetin. It is shown that all the three reagents produce different colorimetric responses that can be detected by a mini-spectrophotometer–monitor calibrator or by a smartphone. The method is applicable to direct measuring high contents of dihydroquercetin (the linearity range is 0.026–1 mg mL⁻¹, and the limit of detection is 7.7 µg mL⁻¹), which is favorable for many dietary supplements. The analysis of a food supplement was possible with the relative standard deviations of 9–26%, which is satisfactory for quantitative and semiquantitative determinations. It was found that plotting a calibration graph in 3D space of the three reagents’ responses allows us to distinguish dihydroquercetin from its close structural analogue, quercetin.

Optical sensors based on silver triangular nanoplates (AgTNPs) are insufficiently studied as probes for the spectrophotometric determination of biologically active compounds. In the present article, an interaction between label-free AgTNPs and bioflavonoids in the presence of silver(I) ions was assessed to outline the possibilities of AgTNPs as a colorimetric probe for the fast and sensitive determination of bioflavonoids. It is shown that the interaction was accompanied by a bathochromic shift of the local surface plasmon resonance band of nanoparticles and an increase in its intensity. Seven bioflavonoids differing in their structure were tested. The influence of the structure of analytes and the main external factors on the analytical signal is discussed in detail. It was found that the detection limits of bioflavonoids in the selected optimal conditions increased in the series morin < rutin = quercetin < taxifolin and came to 0.9, 1.2, 1.2, and 2.0 μmol L⁻¹, respectively. Chrysin, naringenin, and naringin were found not to affect the spectral characteristics of AgTNPs. The suggested approach was applied for the spectrophotometric determination of flavonoids in pharmaceuticals and onion peel.