Daisuike Taniguchi
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Daisuike Taniguchi has a total of 4 co-authors. Here are the top co-authors by number of shared publications:
- Golnaz Karoubi
Latner Thoracic Research Laboratories, University Health Network, Toronto, ON M5G 1L7, Canada
1 shared publication - Mohammadali Ahmadipour
Latner Research Laboratories, Division of Thoracic Surgery, Toronto General Hospital, University Health Network, Toronto, Canada
1 shared publication - Pascal Duchesneau
Latner Thoracic Research Laboratories, DIvision of Thoracic Surgery, 101 College St. 2-817, Toronto, ON, M5G1L7, Canada
1 shared publication - Thomas K. Waddell
Division of Thoracic Surgery, University Health Network, University of Toronto, Toronto, ON, Canada
1 shared publication
Latest Publications
- 1 January 2021
- Published by SAGE Publications in Tissue Engineering Part C: Methods
- Vol. 27 (1) , 1-11
Negative Pressure Cell Delivery Augments Recellularization of Decellularized Lungs
For end-stage lung disease, lung transplantation remains the only treatment but is limited by the availability of organs. Production of bioengineered lungs via recellularization is an alternative but is hindered by inadequate repopulation. We present a cell delivery method via the generation of negative pressure. Decellularized lungs were seeded with human bronchial epithelial cells using gravity-based perfusion or negative pressure (via air removal). After delivery, lungs were maintained in static conditions for 18 hrs, and cell surface coverage was qualitatively assessed using histology and analyzed by subjective scoring and an image analysis software. Negative pressure seeded lungs had higher cell surface coverage area, and this effect was maintained following five days of culture. Enhanced coverage via negative pressure cell delivery was also observed when vasculature seeded with endothelial cells. Our findings show that negative pressure cell delivery is a superior approach for the recellularization of the bioengineered lung.
- 1 January 2021
- Published by SAGE Publications in Tissue Engineering Part C: Methods
- Vol. 27 (1) , 1-11
Negative Pressure Cell Delivery Augments Recellularization of Decellularized Lungs
For end-stage lung disease, lung transplantation remains the only treatment but is limited by the availability of organs. Production of bioengineered lungs via recellularization is an alternative but is hindered by inadequate repopulation. We present a cell delivery method via the generation of negative pressure. Decellularized lungs were seeded with human bronchial epithelial cells using gravity-based perfusion or negative pressure (via air removal). After delivery, lungs were maintained in static conditions for 18 hrs, and cell surface coverage was qualitatively assessed using histology and analyzed by subjective scoring and an image analysis software. Negative pressure seeded lungs had higher cell surface coverage area, and this effect was maintained following five days of culture. Enhanced coverage via negative pressure cell delivery was also observed when vasculature seeded with endothelial cells. Our findings show that negative pressure cell delivery is a superior approach for the recellularization of the bioengineered lung.