Isabel Mateos-White

Neural stem cells (NSCs) sustain neurogenesis within specialized niches, yet how adhesion-dependent mechanisms during development control lifelong behavior remains unclear. Here, we identify Afadin, a core adherens junction protein, as a regulator. Dorsal loss of Afadin during development drives formation of a stable, ventricular-independent ectopic germinal zone in the neocortex, populated by self-renewing, multipotent NSCs sustaining neurogenesis into adulthood. This ectopic niche emerges within a disorganized cortical environment reminiscent of subcortical band heterotopia, a malformation linked to human neurodevelopmental disorders. Concomitantly, the canonical ventricular-subventricular zone (V-SVZ) is disrupted, with persistent NSC activation, altered ependymal specification, ventricular disorganization, and increased neurogenesis. Transcriptomic profiling of the V-SVZ reveals a shift from adhesion- and quiescence-associated programs toward proliferative and neurogenic states through cell-autonomous and non-cell-autonomous mechanisms. Mosaic postnatal or adult deletion confirms a cell-intrinsic role of Afadin in NSC activation. Together, these findings reveal flexibility and plasticity of NSCs across the lifespan.

Stem cells are generally thought to depend on specialized niches that provide signals for their long-term maintenance. Whether stem-cell competence is intrinsically constrained by anatomical organization remains unclear. Here, we show that neural stem cells can establish and sustain functional persistent stem-cell populations outside their normal anatomical context. Using developmental deletion of the cell-adhesion regulator Afadin as a tool to disrupt cortical tissue organization, we find that neural progenitors are displaced from the ventricular surface and establish an ectopic germinal zone (EGZ) into advanced adulthood. EGZ stem-cell populations retain self-renewal and multilineage differentiation capacity throughout this period. In parallel, the ventricular-subventricular zone (V-SVZ) undergoes persistent reorganization of tissue architecture, cellular composition, molecular state, and stem-cell activity. Together, these findings reveal unexpected plasticity in the relationship between stem cells and their tissue environment, suggesting that canonical niche anatomy may constrain where stem cells normally reside without defining the limits of functional stem-cell competence.

Schizophrenia is associated with altered cortical circuitry. Although the schizophrenia risk geneNRG1is known to affect the wiring of inhibitory interneurons, its role in excitatory neurons and axonal development is unclear. Here, we investigated the role of Nrg1 in the development of the corpus callosum, the major interhemispheric connection formed by cortical excitatory neurons. We found that deletion of Nrg1 impaired callosal axon development in vivo. Experiments in vitro and in vivo demonstrated that Nrg1 is cell-autonomously required for axonal outgrowth and that intracellular signaling of Nrg1 is sufficient to promote axonal development in cortical neurons and specifically in callosal axons. Furthermore, our data suggest that Nrg1 signaling regulates the expression of Growth Associated Protein 43, a key regulator of axonal growth. In conclusion, our study demonstrates that NRG1 is involved in the formation of interhemispheric callosal connections and provides a novel perspective on the relevance of NRG1 in excitatory neurons and in the etiology of schizophrenia.

Adult neurogenesis is supported by multipotent neural stem cells (NSCs) with unique properties and growth requirements. Adult NSCs constitute a reversibly quiescent cell population that can be activated by extracellular signals from the microenvironment in which they reside in vivo. Although genomic imprinting plays a role in adult neurogenesis through dose regulation of some relevant signals, the roles of many imprinted genes in the process remain elusive. Insulin-like growth factor 2 (IGF2) is encoded by an imprinted gene that contributes to NSC maintenance in the adult subventricular zone through a biallelic expression in only the vascular compartment. We show here that IGF2 additionally promotes terminal differentiation of NSCs into astrocytes, neurons and oligodendrocytes by inducing the expression of the maternally expressed gene cyclin-dependent kinase inhibitor 1c (Cdkn1c), encoding the cell cycle inhibitor p57. Using intraventricular infusion of recombinant IGF2 in a conditional mutant strain with Cdkn1c-deficient NSCs, we confirm that p57 partially mediates the differentiation effects of IGF2 in NSCs and that this occurs independently of its role in cell-cycle progression, balancing the relationship between astrogliogenesis, neurogenesis and oligodendrogenesis.

The neocortex is an exquisitely organized structure achieved through complex cellular processes from the generation of neural cells to their integration into cortical circuits after complex migration processes. During this long journey, neural cells need to establish and release adhesive interactions through cell surface receptors known as cell adhesion molecules (CAMs). Several types of CAMs have been described regulating different aspects of neurodevelopment. Whereas some of them mediate interactions with the extracellular matrix, others allow contact with additional cells. In this review, we will focus on the role of two important families of cell–cell adhesion molecules (C-CAMs), classical cadherins and nectins, as well as in their effectors, in the control of fundamental processes related with corticogenesis, with special attention in the cooperative actions among the two families of C-CAMs.

The neocortex is an exquisitely organized structure achieved through complex cellular processes from the generation of neural cells to their integration into cortical circuits after complex migration processes. During this long journey, neural cells need to stablish and release adhesive interactions through cell surface receptors known as cell adhesion molecules (CAMs). Several types of CAMs have been described regulating different aspects of neurodevelopment. Whereas some of them mediate interactions with the extracellular matrix, others allow contacts with additional cells. In this review, we will focus on the role of two important families of cell-cell adhesion molecules (C-CAMs), classical cadherins and nectins, as well as in their effectors, in the control of fundamental processes related with corticogenesis, with especial attention in the cooperative actions among the two families of C-CAMs.

In utero electroporation is an in vivo DNA transfer technique extensively used to study the molecular and cellular mechanisms underlying mammalian corticogenesis. This procedure takes advantage of the brain ventricles to allow the introduction of DNA of interest and uses a pair of electrodes to direct the entrance of the genetic material into the cells lining the ventricle, the neural stem cells. This method allows researchers to label the desired cells and/or manipulate the expression of genes of interest in those cells. It has multiple applications, including assays targeting neuronal migration, lineage tracing, and axonal pathfinding. An important feature of this method is its temporal and regional control, allowing circumvention of potential problems related with embryonic lethality or the lack of specific CRE driver mice. Another relevant aspect of this technique is that it helps to considerably reduce the economic and temporal limitations that involve the generation of new mouse lines, which become particularly important in the study of interactions between cell types that originate in distant areas of the brain at different developmental ages. Here we describe a double electroporation strategy that enables targeting of cell populations that are spatially and temporally separated. With this approach we can label different subtypes of cells in different locations with selected fluorescent proteins to visualize them, and/or we can manipulate genes of interest expressed by these different cells at the appropriate times. This strategy enhances the potential of in utero electroporation and provides a powerful tool to study the behavior of temporally and spatially separated cell populations that migrate to establish close contacts, as well as long-range interactions through axonal projections, reducing temporal and economic costs.