Yunqiu Liu

During excavation in a deep tunnel, dynamic disaster is an extremely severe impact failure. The necessity of an energy-absorbing support system is analyzed for different characteristics of dynamic disaster (rockburst) failure. The energy-absorbing support system design includes a combination of early-warning, energy-absorbing bolts, and other components. This support system is designed to meet the energy requirement of a rockburst disaster based on an early warning. The energy-absorbing rockbolt uses the stepwise decoupling technique to realize the brittle-ductile transition of the structure, which is referred to as a stepwise decoupling rockbolt (SD-bolt). The ultimate force, ultimate deformation, and energy were calculated as 241 kN, 442.3 mm, and 95.89 kJ under static pull-out load. Monitored by a microseismic system, the support system was tested by moderate rockburst disaster impact on site. Considering similar rockburst disaster failure cases, this energy-absorbing support system can reduce rockburst disaster damage to a certain extent and improve overall safety during deep engineering construction.

The key to guaranteeing excavation face stability in slurry shield tunneling is the formation of an impermeable dynamic filter cake. At the same time, the effect of the cutter head and rotation speed should be taken into account. We studied the characteristics and formation mechanism of the dynamic filter cake using a newly developed experimental apparatus. The experiment results show that the hysteretic infiltration zone appeared in the curves of stepped loading filtration while the cutter head was rotating, and the volume of water filtration increased by 11.2% compared to when the cutter head stopped. The higher the rotation speed was, the lower the conversion rate of the effective stress was. Under the same rotation speed, the formation time of the 6-cutter arm was almost 5 s slower than that of the 5-cutter arm. As the cutter arms and the rotation speed increased, the stratum’s electrical conductivity increased and stabilized at a distance of 20 cm from the cutter head. The filter cake transited from ‘filter cake plus an infiltration zone’ to ‘an infiltration zone without a filter cake’ with the increase of the rotation speed. The thickness of the dynamic filter cake was smaller than that of the static filter cake, the thickness of 10 groups decreased significantly, and the average thickness decreased by 76.15% at 1.0 rpm. The mesoscopic formation process of the dynamic filter cake can be divided into six stages. This study revealed the slurry penetration mechanism and filter cake characteristics present under cyclic damage by the shield cutter head to the filter cake and soil and provided theoretical support on how to maintain the stability of the excavation face during slurry shield tunneling.

A closed-form analytical solution to rheological phenomena is obtained based on Hoek-Brown (HB) yield criterion and the generalized Bingham model. Once the circular tunnel is excavated, an initial plastic region is formed. When the total stresses fulfil the yield condition, the initial plastic region steps into the viscoplastic region, in which the displacement develops with time. The one-dimensional constitutive equation of the generalized Bingham model is transformed into a three-dimensional expression in polar coordinates by combining the plane strain condition. With the help of equilibrium differential equation, geometric equation and boundary condition, the stress and displacement in the initial plastic region can be expressed. At the moment of t, the stresses and displacements are described similar to the solutions of the elasto-plastic state. The stresses, strains and displacements after transformation can be expressed in the viscoplastic region. The proposed solution was verified by the closed-form solution of circular tunnels in elastic-brittle-plastic rock mass.

The construction of mine surface facilities shall be carried out according to the scope of mining rights and ease of use. The underground mining of Shirengou Iron Mine uses filling method mining, and the filling station needs to be built on the surface. According to the design, it is constructed near the open pit. Based on the protection of the filling station system, the stability analysis of the open slope is needed. The failure mode of iron ore open slope is circular arc sliding failure mode. The calculation model of the stability analysis is calculated by the residual thrust method. The maximum residual thrust of the I-I section is 128.9 tons/meter, and the maximum residual thrust of the II-II section is 75.39 tons/meter. According to the calculation results, the safety measures such as slope dressing and masonry retaining wall are carried out on the open slope, and the grouting prestressed anchor cable is selected to reinforce the slope. The anchor cables are connected by reinforced concrete frame and beam, and the concrete is used. The beam acts as an anchor pier. In order to effectively consolidate the slope stability, the grouting method is combined to effectively treat the slope. After a series of slope reinforcement measures, the stability of the open slope and the safety of the filling station can be guaranteed.

Melting temperature shift (Tm-shift) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a Tm-shift method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5 ́ end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of Tm-shift was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The Tm-shift method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of Tm-values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was 5.22×10-6 and 5.28×10-6 ng/μl samples of AceP and AtuP, respectively. The accuracy of Tm-shift method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the Tm-shift detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.