Norman Pfeiffer
Current Organizations / Institutes
The organizations/institutes from the scholar's latest article
Research Subjects
Norman Pfeiffer has a total of 33 co-authors. Here are the top co-authors by number of shared publications:
- Abdelhamid Errachid
Institute of Analytical Sciences, University of Lyon, 69100 Villeurbanne, France
4 shared publications - Joan Bausells
Institute of Microelectronics of Barcelona (IMB-CNM, CSIC), Campus UAB, 08193 Bellaterra, Spain
4 shared publications - Abdelhamid Elaissari
Universite Claude Bernard Lyon 1, ISA, UMR 5280, CNRS, 5 Rue de la Doua, 69100 Villeurbanne, France
3 shared publications Albert Heuberger
Information Technology (LIKE), Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91058 Erlangen, Germany
3 shared publicationsHamdi Ben Halima
Institute of Analytical Sciences (ISA) - UMR 5280, Claude Bernard Lyon 1 University (UCBL), 69100, Lyon, France
3 shared publications- Albert Alcacer
Centro Nacional de Microelectrónica (IMB-CSIC) Campus UAB, Bellaterra, 08193 Barcelona, Spain
2 shared publications
Latest Publications
- Open Access
Portable Impedance Analyzer for FET-Based Biosensors with Embedded Analysis of Randles Circuits’ Spectra
The electrochemical impedance spectroscopy (EIS) is a measurement method for characterizing bio-recognition events of a sensor, such as field-effect transistor-based biosensors (BioFETs). Due to the lack of portable impedance spectroscopes, EIS applies mainly in laboratories preventing application-oriented use in the field. This work presents a portable impedance analyzer (PIA) providing a 4-channel EIS of BioFETs. It performs the analysis of the recorded spectra by determining the charge transfer resistance with a power-saving algorithm. Therefore, a circle is fitted into the Nyquist representation of the Randles circuit, from whose zero crossings can be determined. The introduced algorithm was evaluated on 100 simulated spectra of Randles circuits. To analyze the overall system, an adjustable reference circuit was developed that simulates configurable Randles circuits. Additional measurements with pH-sensitive ion-sensitive field-effect transistors (ISFETs) demonstrate the application of the measurement system with electrochemical sensors. Using simulated spectra, the circular fitting is able to detect with a median accuracy of at an average nominal power of 40 mW and 3054 µs computing time. The PIA with the embedded implementation of the circuit fitting achieves a median error for Rct of 4.2% using the introduced Randles circuit simulator (RCS). Measurements with ISFETs show deviations of 6.5 ± 2.8% compared to the complex non-linear least squares (CNLS), but is significantly faster and more efficient. The presented system allows a portable, power-saving performance of EIS. Future optimizations for a specific applications can improve the presented system and enable novel low-power and automated measurements of biosensors outside the laboratory.Open AccessPortable Impedance Analyzer for FET-Based Biosensors with Embedded Analysis of Randles Circuits’ Spectra
The electrochemical impedance spectroscopy (EIS) is a measurement method for characterizing bio-recognition events of a sensor, such as field-effect transistor-based biosensors (BioFETs). Due to the lack of portable impedance spectroscopes, EIS applies mainly in laboratories preventing application-oriented use in the field. This work presents a portable impedance analyzer (PIA) providing a 4-channel EIS of BioFETs. It performs the analysis of the recorded spectra by determining the charge transfer resistance with a power-saving algorithm. Therefore, a circle is fitted into the Nyquist representation of the Randles circuit, from whose zero crossings can be determined. The introduced algorithm was evaluated on 100 simulated spectra of Randles circuits. To analyze the overall system, an adjustable reference circuit was developed that simulates configurable Randles circuits. Additional measurements with pH-sensitive ion-sensitive field-effect transistors (ISFETs) demonstrate the application of the measurement system with electrochemical sensors. Using simulated spectra, the circular fitting is able to detect with a median accuracy of at an average nominal power of 40 mW and 3054 µs computing time. The PIA with the embedded implementation of the circuit fitting achieves a median error for Rct of 4.2% using the introduced Randles circuit simulator (RCS). Measurements with ISFETs show deviations of 6.5 ± 2.8% compared to the complex non-linear least squares (CNLS), but is significantly faster and more efficient. The presented system allows a portable, power-saving performance of EIS. Future optimizations for a specific applications can improve the presented system and enable novel low-power and automated measurements of biosensors outside the laboratory. - Open Access
- 1 May 2025
- Published by Elsevier in Measurement
- Vol. 253
Methods for microsecond accuracy synchronization of Wireless Body Area Networks for biosignal acquisition using Bluetooth Low Energy
- 1 May 2025
- Published by Elsevier in Measurement
- Vol. 253
Open AccessMethods for microsecond accuracy synchronization of Wireless Body Area Networks for biosignal acquisition using Bluetooth Low Energy
- Open Access
- 21 February 2024
- Published by MDPI AG in Micromachines
- Vol. 15 (3) , 296
An ImmunoFET Coupled with an Immunomagnetic Preconcentration Technique for the Sensitive EIS Detection of HF Biomarkers
We propose a new strategy using a sandwich approach for the detection of two HF biomarkers: tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10). For this purpose, magnetic nanoparticles (MNPs) (MNPs@aminodextran) were biofunctionalized with monoclonal antibodies (mAbs) using bis (sulfosuccinimidyl) suberate (BS3) as a cross-linker for the pre-concentration of two biomarkers (TNF-α and IL-10). In addition, our ISFETs were biofunctionalized with polyclonal antibodies (pAbs) (TNF-α and IL-10). The biorecognition between pAbs immobilized on the ISFET and the pre-concentrate antigen (Ag) on MNPs was monitored using electrochemical impedance spectroscopy (EIS). Our developed ImmunoFET showed a low detection limit (0.03 pg/mL) toward our target analyte when compared to previously published electrochemical immunosensors. It showed a higher sensitivity than for other HF biomarkers. Finally, the standard addition method was used to determine the unknown concentration in artificial saliva. The results matched with the expected values well.
- 21 February 2024
- Published by MDPI AG in Micromachines
- Vol. 15 (3) , 296
Open AccessAn ImmunoFET Coupled with an Immunomagnetic Preconcentration Technique for the Sensitive EIS Detection of HF Biomarkers
We propose a new strategy using a sandwich approach for the detection of two HF biomarkers: tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10). For this purpose, magnetic nanoparticles (MNPs) (MNPs@aminodextran) were biofunctionalized with monoclonal antibodies (mAbs) using bis (sulfosuccinimidyl) suberate (BS3) as a cross-linker for the pre-concentration of two biomarkers (TNF-α and IL-10). In addition, our ISFETs were biofunctionalized with polyclonal antibodies (pAbs) (TNF-α and IL-10). The biorecognition between pAbs immobilized on the ISFET and the pre-concentrate antigen (Ag) on MNPs was monitored using electrochemical impedance spectroscopy (EIS). Our developed ImmunoFET showed a low detection limit (0.03 pg/mL) toward our target analyte when compared to previously published electrochemical immunosensors. It showed a higher sensitivity than for other HF biomarkers. Finally, the standard addition method was used to determine the unknown concentration in artificial saliva. The results matched with the expected values well.
- 1 January 2022
- Published by Springer Nature
- p. 61-79
Determination of Charge Transfer Resistance from Randles Circuit Spectra Using Elliptical Fitting
- 1 January 2022
- Published by Springer Nature
- p. 61-79
Determination of Charge Transfer Resistance from Randles Circuit Spectra Using Elliptical Fitting
- 1 May 2021
- Published by Elsevier in Analytica Chimica Acta
- Vol. 1161, 338468
A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring
- 1 May 2021
- Published by Elsevier in Analytica Chimica Acta
- Vol. 1161, 338468
A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring
- Open Access
- 8 December 2017
- Published by MDPI AG in Applied Sciences
- Vol. 7 (12) , 1277
Wearable Current-Based ECG Monitoring System with Non-Insulated Electrodes for Underwater Application
The second most common cause of diving fatalities is cardiovascular diseases. Monitoring the cardiovascular system in actual underwater conditions is necessary to gain insights into cardiac activity during immersion and to trigger preventive measures. We developed a wearable, current-based electrocardiogram (ECG) device in the eco-system of the FitnessSHIRT platform. It can be used for normal/dry ECG measuring purposes but is specifically designed to allow underwater signal acquisition without having to use insulated electrodes. Our design is based on a transimpedance amplifier circuit including active current feedback. We integrated additional cascaded filter components to counter noise characteristics specific to the immersed condition of such a system. The results of the evaluation show that our design is able to deliver high-quality ECG signals underwater with no interferences or loss of signal quality. To further evaluate the applicability of the system, we performed an applied study with it using 12 healthy subjects to examine whether differences in the heart rate variability exist between sitting and supine positions of the human body immersed in water and outside of it. We saw significant differences, for example, in the RMSSD and SDSD between sitting outside the water (36 ms) and sitting immersed in water (76 ms) and the pNN50 outside the water (6.4%) and immersed in water (18.2%). The power spectral density for the sitting positions in the TP and HF increased significantly during water immersion while the LF/HF decreased significantly. No significant changes were found for the supine position.
- 8 December 2017
- Published by MDPI AG in Applied Sciences
- Vol. 7 (12) , 1277
Open AccessWearable Current-Based ECG Monitoring System with Non-Insulated Electrodes for Underwater Application
The second most common cause of diving fatalities is cardiovascular diseases. Monitoring the cardiovascular system in actual underwater conditions is necessary to gain insights into cardiac activity during immersion and to trigger preventive measures. We developed a wearable, current-based electrocardiogram (ECG) device in the eco-system of the FitnessSHIRT platform. It can be used for normal/dry ECG measuring purposes but is specifically designed to allow underwater signal acquisition without having to use insulated electrodes. Our design is based on a transimpedance amplifier circuit including active current feedback. We integrated additional cascaded filter components to counter noise characteristics specific to the immersed condition of such a system. The results of the evaluation show that our design is able to deliver high-quality ECG signals underwater with no interferences or loss of signal quality. To further evaluate the applicability of the system, we performed an applied study with it using 12 healthy subjects to examine whether differences in the heart rate variability exist between sitting and supine positions of the human body immersed in water and outside of it. We saw significant differences, for example, in the RMSSD and SDSD between sitting outside the water (36 ms) and sitting immersed in water (76 ms) and the pNN50 outside the water (6.4%) and immersed in water (18.2%). The power spectral density for the sitting positions in the TP and HF increased significantly during water immersion while the LF/HF decreased significantly. No significant changes were found for the supine position.