Bin Wang

In this study, our aim is to find an effective method to solve the problem of disease similarity caused by multiple diseases occurring on the same leaf. This study proposes the use of an optimized RegNet model to identify seven common apple leaf diseases. We conducted comparisons and analyses on the impact of various factors, such as training methods, data expansion methods, optimizer selection, image background, and other factors, on model performance. The findings suggest that utilizing offline expansion and transfer learning to fine-tune all layer parameters can enhance the model’s classification performance, while complex image backgrounds significantly influence model performance. Additionally, the optimized RegNet network model demonstrates good generalization ability for both datasets, achieving testing accuracies of 93.85% and 99.23%, respectively. These results highlight the potential of the optimized RegNet network model to achieve high-precision identification of different diseases on the same apple leaf under complex field backgrounds. This will be of great significance for intelligent disease identification in apple orchards in the future.

Cerasus Humilis fruit is susceptible to rapid color changes post-harvest, which degrades its quality. This research utilized hyperspectral imaging technology to detect and visually analyze the soluble solid content (SSC) and firmness of the fruit, aiming to improve quality and achieve optimal pricing. Four maturity stages (color turning stage, coloring stage, maturity stage, and fully ripe stage) of Cerasus Humilis fruit were examined using hyperspectral images (895–1700 nm) alongside data collection on SSC and firmness. These samples were divided into a calibration set and a validation set with a ratio of 3:1 by sample set partitioning based on the joint X-Y distances (SPXY) method. The original spectral data was processed by a spectral preprocessing method. Multiple linear regression (MLR) and nonlinear least squares support vector machine (LS-SVM) detection models were established using feature wavelengths selected by the successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS), uninformative variable elimination (UVE), and two combined downscaling algorithms (UVE-SPA and UVE-CARS), respectively. For SSC and firmness detection, the best models were the SNV-SPA-LS-SVM model with 18 feature wavelengths and the original spectra-UVE-CARS-LS-SVM model with eight feature wavelengths, respectively. For SSC, the correlation coefficient of prediction (Rp) was 0.8526, the root mean square error of prediction (RMSEP) was 0.9703, and the residual prediction deviation (RPD) was 1.9017. For firmness, Rp was 0.7879, RMSEP was 1.1205, and RPD was 2.0221. Furthermore, the optimal model was employed to retrieve the distribution of SSC and firmness within Cerasus Humilis fruit. This retrieved information facilitated visual inspection, enabling a more intuitive and comprehensive assessment of SSC and firmness at each pixel level. These findings demonstrated the effectiveness of hyperspectral imaging technology for determining SSC and firmness in Cerasus Humilis fruit. This paves the way for online monitoring of fruit quality, ultimately facilitating timely harvesting.

Detection of skin defects in Cerasus humilis fruit is a critical process to guarantee its quality and price. This study presents a valid method for the detection of defective features in Cerasus humilis fruits based on hyperspectral imaging. A total of 420 sample images were acquired that included three types of natural defects and undamaged samples. After acquiring hyperspectral images of Cerasus humilis fruits, the spectral data were extracted from the region of interest (ROI). Five spectral preprocessing methods were used to preprocess the original spectral data, including Savitsky–Golay (S-G), standard normal variate (SNV), multiplicative scatter correction (MSC), baseline correction (BC), and de-trending (De-T). Regression coefficient (RC), successive projections algorithm (SPA), and competitive adaptive reweighed sampling (CARS) were conducted to select optimal sensitive wavelengths (SWs); as a result, 11 SWs, 17 SWs, and 13 SWs were selected, respectively. Then, the least squares-support vector machine (LS-SVM) discrimination model was established using the selected SWs. The results showed that the discriminate accuracy of the CARS-LS-SVM method was 91.43%. Based on the characteristics of image information, images corresponding to eight sensitive wavebands (950, 994, 1071, 1263, 1336, 1457, 1542, and 1628 nm) selected by CARS were subjected to principal component analysis (PCA). Then, an effective approach for detecting the defective features was exploited based on the imfill function, canny operator, region growing algorithm, bwareaopen function, and the images of PCA. The location and area of defect feature of 105 Cerasus humilis fruits could be recognized; the detect precision was 88.57%. This investigation demonstrated that hyperspectral imaging combined with an image processing technique could achieve the rapid identification of undamaged samples and natural defects in Cerasus humilis fruit. This provides a theoretical basis for the development of Cerasus humilis fruit grading and sorting equipment.

The accurate segmentation of apple leaf disease spots is the key to identifying the classification of apple leaf diseases and disease severity. Therefore, a DeepLabV3+ semantic segmentation network model with an actors spatial pyramid pool module (ASPP) was proposed to achieve effective extraction of apple leaf lesion features and to improve the apple leaf disease recognition and disease severity diagnosis compared with the classical semantic segmentation network models PSPNet and GCNet. In addition, the effects of the learning rate, optimizer, and backbone network on the performance of the DeepLabV3+ network model with the best performance were analyzed. The experimental results show that the mean pixel accuracy (MPA) and mean intersection over union (MIoU) of the model reached 97.26% and 83.85%, respectively. After being deployed into the smartphone platform, the detection time of the detection system was 9s per image for the portable and intelligent diagnostics of apple leaf diseases. The transfer learning method provided the possibility of quickly acquiring a high-performance model under the condition of small datasets. The research results can provide a precise guide for the prevention and precise control of apple diseases in fields.

The intelligent identification and classification of plant diseases is an important research objective in agriculture. In this study, in order to realize the rapid and accurate identification of apple leaf disease, a new lightweight convolutional neural network RegNet was proposed. A series of comparative experiments had been conducted based on 2141 images of 5 apple leaf diseases (rust, scab, ring rot, panonychus ulmi, and healthy leaves) in the field environment. To assess the effectiveness of the RegNet model, a series of comparison experiments were conducted with state-of-the-art convolutional neural networks (CNN) such as ShuffleNet, EfficientNet-B0, MobileNetV3, and Vision Transformer. The results show that RegNet-Adam with a learning rate of 0.0001 obtained an average accuracy of 99.8% on the validation set and an overall accuracy of 99.23% on the test set, outperforming all other pre-trained models. In other words, the proposed method based on transfer learning established in this research can realize the rapid and accurate identification of apple leaf disease.

In this study, the changes of total flavonoids content in Cerasus Humilis fruits during storage periods were detected by using hyperspectral imaging (HSI) technique over the spectral region of 895–1,700 nm, and the spatial distribution of flavonoids was visualized during 24 days storage periods. The hyperspectral images of 240 samples of Cerasus Humilis were collected at different storage times (0, 8, 16, and 24 days). The Monte‐Carlo outlier detection method was applied to identify four abnormal samples, three spectral preprocessing methods were used to preprocess the original spectral data, including savitzky–golay, standard normal variate, and baseline correction (BC). The x‐loading weights, competitive adaptive reweighed sampling (CARS), uninformative variable elimination (UVE), UVE‐CARS and the combination of UVE and successive projections algorithm (UVE‐SPA) were used to reduce the dimension of spectral data and extract the characteristic wavelength, respectively. Then multiple linear regression and nonlinear least squared support vector machine (LS‐SVM) regression models were developed based on full‐spectrum data and selected characteristic wavelength. The results showed that BC was the best preprocessing approach, the LS‐SVM models based on the nine effective wavelengths selected by UVE‐CARS achieved the best results with correlation coefficient of prediction of .9357, root mean square error of prediction of 2.0107, and residual prediction deviation of 2.2809, respectively. The overall results demonstrated that the HSI technology coupled with chemometric algorithms is feasible to determine total flavonoids content, UVE‐CARS‐LS‐SVM model was the optimal model. Visualization of the total flavonoids content during storage periods distribution map was performed, which clearly showed that the total flavonoids content kept decreasing with storage time.Practical Applications: Cerasus Humilis is a fruit tree resource endemic to China. The content of total flavonoids is one of the key factors that affect the edible quality of Cerasus Humilis fruit. With the increase of storage time, the active components of flavonoids are easy to be degraded by oxidation, the quality and commercial value of Cerasus Humilis fruit were negatively affected. Thus, the detection of total flavonoids content during the storage of Cerasus Humilis fruit is of great significance. Traditional methods for determining total flavonoids content (such as the spectrophotometric techniques method) are mostly destructive, inefficient, time‐consuming, and environmental pollution, which cannot meet the requirements of online monitoring of total flavonoids content. Hyperspectral imaging (HSI) technology has the advantages of rapid, nondestructive, accurate, and nonpollution. The results indicated that the HSI technology coupled with chemometric algorithms could be used to predict the changes of the total flavonoids content in Cerasus Humilis fruit during storage periods, which provided a theoretical basis for online and real‐time monitoring of the quality of Cerasus Humilis fruit during storage.

Deep learning is a branch of artificial intelligence. In recent years, with the advantages of automatic learning and feature extraction, it has been widely concerned by academic and industrial circles. It has been widely used in image and video processing, voice processing, and natural language processing. At the same time, it has also become a research hotspot in the field of agricultural plant protection, such as plant disease recognition and pest range assessment, etc. The application of deep learning in plant disease recognition can avoid the disadvantages caused by artificial selection of disease spot features, make plant disease feature extraction more objective, and improve the research efficiency and technology transformation speed. This review provides the research progress of deep learning technology in the field of crop leaf disease identification in recent years. In this paper, we present the current trends and challenges for the detection of plant leaf disease using deep learning and advanced imaging techniques. We hope that this work will be a valuable resource for researchers who study the detection of plant diseases and insect pests. At the same time, we also discussed some of the current challenges and problems that need to be resolved.